[1]王照华,杨 越,徐景超,等.家兔股骨植入可降解型镁合金加压螺钉的生物效应与抗菌性能研究[J].医学信息,2021,34(19):88-91.[doi:10.3969/j.issn.1006-1959.2021.19.022]
　WANG Zhao-hua,YANG Yue,XU Jing-chao,et al.Observation on Biological Effects and Antibacterial Properties of Degradable Magnesium Alloycompression Screws Implanted into Rabbit Femur[J].Medical Information,2021,34(19):88-91.[doi:10.3969/j.issn.1006-1959.2021.19.022]
点击复制

家兔股骨植入可降解型镁合金加压螺钉的生物效应与抗菌性能研究()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年19期
页码:
88-91
栏目:
论著
出版日期:
2021-10-01

文章信息/Info

Title:
Observation on Biological Effects and Antibacterial Properties of Degradable Magnesium Alloycompression Screws Implanted into Rabbit Femur
文章编号:
1006-1959（2021）19-0088-04
作者:
王照华1; 杨 越2; 徐景超2; 张雁儒1
河南理工大学医学院1，骨科研究所2，河南 焦作 454000
Author(s):
WANG Zhao-hua1; YANG Yue2; XU Jing-chao2; ZHANG Yan-ru1
Medical College1,Institute of Orthopedics2,Henan University of Technology,Jiaozuo 454000,Henan,China
关键词:
镁合金; 家兔; 生物安全性; 新型材料抗菌性; 金黄色葡萄球菌
Keywords:
Magnesium alloy; Rabbit; Biosafety; New materials antimicrobial properties; Staphylococcus aureus
分类号:
R687.3+2
DOI:
10.3969/j.issn.1006-1959.2021.19.022
文献标志码:
A
摘要:
目的 评价一种新型可降解镁合金螺钉作为植入物在家兔股骨干上的生物安全性、降解性与抗菌性。方法 选取成年家兔20只，随机分为对照组与实验组，各10只。实验组行麻醉后在股骨骨干钻孔后向骨髓腔内注射入金黄色葡萄球菌菌液并加压植入镁合金螺钉固定，对照组与上述手术处理相同，但不植入镁合金螺钉。60 d后对实验家兔行影像学、组织切片、金黄色葡萄球菌检测及离体股骨形态观察。结果 镁合金螺钉降解后的股骨形态：60 d后螺钉完全降解消失，原植入部位形成膨大的骨痂，周围组织无坏死无炎症；影像学检测：术后30 d时可观察到螺钉开始降解且固定良好，术后60 d螺钉已完全消失，周围骨组织无坏死样症状；骨组织切片检测：染色后观察可见植入部位成骨细胞增加，骨细胞增生呈无序排列；金黄色葡萄球菌检测：实验组金黄色葡萄球菌阴性率90.00%，对照组金黄色葡萄球菌阴性率20.00%。结论 新型可降解镁合金螺钉作为骨科固定物，在生物降解吸收方面与抗菌方面展现出优良的效果，其具有生物安全性高、降解完全、促进骨生长、避免二次手术等优良特性。
Abstract:
Objective To evaluate the biosafety， degradability and antimicrobial activity of a new degradable magnesium alloy screw as an implant on the femoral shaft of rabbits.Methods Totally 20 adult rabbits were randomly divided into the experimental group and the control group with 10 rabbits each. After anesthesia, the experimental group was injected with Staphylococcus aureus bacterial solution into the bone marrow cavity after drilling the shaft of the femur， and magnesium alloy screws were inserted into the bone marrow cavity under pressure for fixation. The control group underwent the same surgical treatment as above, but no magnesium alloy screws were implanted. Sixty days later, imaging， histological section, Staphylococcus aureus detection and the morphology of femur in vitro were observed.Results The morphology of femur after magnesium alloy screw degradation: 60 d later, the screw completely degraded and disappeared, and the original implant site formed an enlarged callus without necrosis and inflammation in the surrounding tissues. Imaging detection: 30 d after the operation, the screw began to degrade and was well fixed; 60 d after the operation, the screw completely disappeared, and the surrounding bone tissue had no necrosis symptoms. Detection of bone tissue sections: the bone cells were increased and the proliferation of bone cells was disordered after staining. Staphylococcus aureus detection: the experimental group Staphylococcus aureus negative rate of 90.00%， the control group Staphylococcus aureus negative rate of 20.00%.Conclusion The new biodegradable magnesium alloy screws as orthopedic fixation have shown excellent effects in biodegradable absorption and antibacterial， with high biological safety， complete degradation， promoting bone growth， avoiding secondary surgery and other excellent characteristics.

参考文献/References:

[1]徐亦驰,尹合勇,孙振,等.AZ31镁合金材料植入物在兔股骨髁内的降解:Micro-CT评价[J].中国组织工程研究,2016,20(16):2303-2309.[2]Bakhsheshi-Rad HR,Hamzah E,Ismail AF,et al.Bioactivity, in-vitro corrosion behavior, and antibacterial activity of silver-zeolites doped hydroxyapatite coating on magnesium alloy[J]. Transactions of Nonferrous Metals Society of China,2018,28(8):1553-1562.[3]Moravej M,Mantovani D.Biodegradable metals for cardiovascular stent application: interests and new opportunities[J].Int J Mol Sci,2011,12(7):4250-4270.[4]马瑞,王家麟,李永伟,等.含镁多孔支架材料的体外抗菌活性和生物相容性[J].中国组织工程研究,2020,24(22):3534-3539.[5]官星旻.可降解生物医用镁合金JDBM作为颌骨修复材料的应用研究[D].上海:上海交通大学,2015.[6]丁大连,于进涛,李鹏,等.实验动物标准颞骨切片规范[J].中华耳科学杂志,2015(1):1-6.[7]王晓宇,齐君,孟宪杰,等.不同固定液对冷冻组织病理切片HE染色质量比较[J].中国法医学杂志,2016,31(2):165-167.[8]毛彦杰,韩培,詹玉林,等.应用有限元分析优化设计应用于踝关节骨折可吸收镁合金螺钉的研究[J].国际骨科学杂志,2017,38(3):200-204.[9]王湛,杨军,李建军.植入体内新型镁合金材料的抗菌性能[J].中国组织工程研究,2018,22(22):3451-3455.[10]张鹏,周立波,张慧明,等.镁合金微弧氧化掺杂Zn-HA复合涂层细胞相容性与抗菌性[J].口腔医学研究,2019,35(10):1001-1004.[11]孔祥东,熊希璐,袁绍峰,等.可降解镁合金AZ31组织夹在新西兰兔体内的实验研究[J].昆明医科大学学报, 2019,40(3):24-29.[12]商晓盼,李涛,陆雨桐,等.AZ31B镁合金降解后周围微环境对成骨细胞生物活性的影响[J].中国医学物理学杂志,2017,34(9):946-949.[13]王湛,杨军,李建军.新型骨科植入物镁合金材料抗菌性能的体外实验研究[J].中国医科大学学报,2014,43(12):1142-1144.[14]杨姗,燕蛟,李斌.温度和酸碱度对金黄色葡萄球菌生长对数期的影响[J].山东化工,2018,47(3):28-30.[15]Mwaanga P,Carraway ER,van den Hurk P.The induction of biochemical changes in Daphnia magna by CuO and ZnO nanoparticles[J].Aquatic Toxicology,2014(150):201-209.

相似文献/References:

[1]张亚男,谢 文,肖 鹏,等.可降解镁合金骨植入对大鼠肝肾功能影响的实验研究[J].医学信息,2022,35(07):92.[doi:10.3969/j.issn.1006-1959.2022.07.022]
　ZHANG Ya-nan,XIE Wen,XIAO Peng,et al.Experimental Study on the Effects of Degradable Magnesium Alloy Bone Implantation on Blood Routine and Liver and Kidney Function in Rats[J].Medical Information,2022,35(19):92.[doi:10.3969/j.issn.1006-1959.2022.07.022]

更新日期/Last Update: 1900-01-01