[1]徐晓雪.CD4单阳参考品辅助检测九色标记人血样品的流式分析技术[J].医学信息,2021,34(20):90-93.[doi:10.3969/j.issn.1006-1959.2021.20.022]
　XU Xiao-xue.Flow Pattern Analysis Technique for CD4 Single-positive Reference Substance-assisted Detection of Nine-color Labeled Human Blood Samples[J].Medical Information,2021,34(20):90-93.[doi:10.3969/j.issn.1006-1959.2021.20.022]
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CD4单阳参考品辅助检测九色标记人血样品的流式分析技术()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年20期
页码:
90-93
栏目:
论著
出版日期:
2021-10-20

文章信息/Info

Title:
Flow Pattern Analysis Technique for CD4 Single-positive Reference Substance-assisted Detection of Nine-color Labeled Human Blood Samples
文章编号:
1006-1959（2021）20-0090-04
作者:
徐晓雪
首都医科大学中心实验室，北京 100069
Author(s):
XU Xiao-xue
Central Laboratory of Capital Medical University,Beijing 100069,China
关键词:
流式细胞术; 荧光补偿; 单细胞分析; 荧光定量; 淋巴细胞
Keywords:
Flow cytometry; Fluorescence compensation; Single cell analysis; Fluorescence quantification; Lymphocytes
分类号:
Q2-33
DOI:
10.3969/j.issn.1006-1959.2021.20.022
文献标志码:
A
摘要:
目的 探索流式多色分析中确定检测器最优参数的客观方法。方法 以BD FACSymphony流式细胞仪检测CD3、CD4、CD8、CD25、CD27、CD45RA、CD56、CD127、CD197九色标记人外周血样品，选用中等表达强度各荧光素标记CD4单阳对照品，以递增检测参数重复分析获得各通道检测器性能曲线，计算获得流式仪器检测优化参数，再以优化参数完成样品检测和补偿调节。结果 补偿前检测群体模糊不清，无法确定检测数据质量；补偿后可见细胞群体的位移，各通道荧光溢漏扩散均较理想，阴性、阳性群体区分明显，各细胞群体指标显示清晰。结论 在多参数流式分析中可以采用适当强度光谱匹配的单阳样品客观、快速的确定检测参数，进而完成补偿调节，可避免操作人员主观判断失误，提高检测质量。
Abstract:
Objective To explore the method to determine the optimal parameters of detector in polychromatic flow cytometry.Methods CD3, CD4, CD8, CD25, CD27, CD45RA, CD56, CD127, CD197 in human peripheral blood were detected by BD FACSymphony flow cytometry. CD4 single positive control was labeled with moderate expression of each fluorescein. The performance curve of each channel detector was obtained by repeated analysis with increasing detection parameters. The optimized parameters of flow cytometry instrument were calculated, and then the sample detection and compensation adjustment were completed with the optimized parameters.Results The detection group before compensation was ambiguous and did not determine the quality of detection data. After compensation, the displacement of cell population was visible, and the fluorescence leakage diffusion of each channel was ideal. The negative and positive groups were clearly distinguished, and the indicators of each cell population were clear.Conclusion In the multi-parameter flow pattern analysis, the single-positive sample with appropriate intensity spectrum matching can be used to determine the detection parameters objectively and quickly, and then complete the compensation adjustment, which can avoid the subjective judgment error of operators and improve the detection quality.

参考文献/References:

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更新日期/Last Update: 1900-01-01