[1]黄光明,赵兴昌,符显昭.水中运动联合中药内治对糖尿病大鼠心肌细胞凋亡和心功能的影响[J].医学信息,2022,35(11):72-76.[doi:10.3969/j.issn.1006-1959.2022.11.020]
　HUANG Guang-ming,ZHAO Xing-chang,FU Xian-zhao.Effects of Aquatic Therapeutic Exercise Combined with Traditional Chinese Medicine Internal Treatment on Cardiomyocyte Apoptosis and Cardiac Function in Diabetic Rats[J].Medical Information,2022,35(11):72-76.[doi:10.3969/j.issn.1006-1959.2022.11.020]
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水中运动联合中药内治对糖尿病大鼠心肌细胞凋亡和心功能的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年11期
页码:
72-76
栏目:
论著
出版日期:
2022-06-01

文章信息/Info

Title:
Effects of Aquatic Therapeutic Exercise Combined with Traditional Chinese Medicine Internal Treatment on Cardiomyocyte Apoptosis and Cardiac Function in Diabetic Rats
文章编号:
1006-1959（2022）11-0072-05
作者:
黄光明; 赵兴昌; 符显昭
（1.右江民族医学院临床医学院，广西 百色 533000；2.右江民族医学院附属医院中医科，广西 百色 533000）
Author(s):
HUANG Guang-ming; ZHAO Xing-chang; FU Xian-zhao
(1.Clinical Medical College,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;2.Department of Traditional Chinese Medicine,the Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)
关键词:
糖尿病; 内质网应激; 水中运动治疗; 中药内治; 心脏重塑; 康复治疗; 心力衰竭
Keywords:
Diabetes; Endoplasmic reticulum stress; Aquatic therapeutic exercise; Traditional Chinese medicine internal treatment; Cardiac remodeling; Rehabilitation treatment; Heart failure
分类号:
R542.2
DOI:
10.3969/j.issn.1006-1959.2022.11.020
文献标志码:
A
摘要:
目的 观察水中运动联合中药内治对糖尿病大鼠心肌细胞凋亡和心功能的影响。方法 SD雄性大鼠125只，随机均分为空白组、模型组、水中运动组、中药内治组、水中运动联合中药内治组（综合康复组），每组25只。除空白组外，其余组利用链脲佐菌素+高脂饲养构建二型糖尿病大鼠模型。造模成功后，各治疗组接受相应药物与运动方案，8周后，超声心动图检测各组LVEDD、LVESD及EF、E/A；RT-PCR检测各组心肌细胞内质网应激标志物GRP78和Caspase-12的mRNA转录水平；TUNEL法检测各组心肌细胞凋亡水平。结果 模型组LVEDD、LVESD 水平高于空白组，E/A、EF均低于空白组，差异有统计学意义（P＜0.05）；模型组GRP78、Caspase-12 mRNA转录水平和心肌细胞凋亡水平高于空白组，统计学意义显著（P＜0.01）；水中运动组、中药内治组、综合康复组LVEDD、LVESD水平低于模型组，E/A、EF高于模型组（P＜0.05）；三组GRP78及Caspase-12mRNA转录水平、心肌细胞凋亡水平均低于模型组（P＜0.05）；综合康复组各项指标改善最为明显，与水中运动组各项指标比较，差异有统计学意义（P＜0.05）。结论 水中运动联合中药内治可降低心肌细胞凋亡水平，抑制心脏重塑，保护心功能，其机制可能与减轻心肌细胞内质网应激，抑制Caspase-12凋亡通路有关。
Abstract:
Objective To observe the effects of aquatic therapeutic exercise combined with traditional chinese medicine internal treatment on cardiomyocyte apoptosis and cardiac function in type 2 diabetic rats.Methods A total of 125 healthy SD male rats were randomly divided into blank group，model group，aquatic therapeutic exercise group，traditional Chinese medicine internal treatment group, aquatic therapeutic exercise group combined traditional Chinese medicine internal treatment group (comprehensive rehabilitation group) with 25 rats in each group. In addition to the blank group, the rat model of type 2 diabetes was established by streptozocin+high fat feeding. After successful modeling, each treatment group received corresponding drugs and exercise programs. After 8 weeks, LVEDD, LVESD, EF and E / A were detected by echocardiography. RT-PCR was used to detect the mRNA transcription levels of endoplasmic reticulum stress markers GRP78 and Caspase-12 in each group. TUNEL method was used to detect the apoptosis level of cardiomyocytes in each group.Results The levels of LVEDD and LVESD in the model group were higher than those in the blank group, and E/A and EF were lower than those in the blank group, with significant difference (P<0.05). The transcription levels of GRP78 and Caspase-12 mRNA and myocardial cell apoptosis in the model group were higher than those in the blank group, and the statistical significance was significant (P<0.01). The levels of LVEDD and LVESD in the aquatic therapeutic exercise group, traditional Chinese medicine internal treatment group and comprehensive rehabilitation group were lower than those in the model group, and the E/A and EF were higher than those in the model group (P<0.05). The levels of GRP78 and Caspase-12 mRNA transcription and cardiomyocyte apoptosis in the three groups were lower than those in the model group (P<0.05). The improvement of each index in the comprehensive rehabilitation group was the most obvious, and the difference was statistically significant compared with that in the water exercise group (P<0.05).Conclusion Aquatic therapeutic exercise combined with traditional Chinese medicine internal treatment can reduce the apoptosis of myocardial cells, inhibit cardiac remodeling and protect cardiac function. The mechanism is related to reducing endoplasmic reticulum stress in myocardial cells and inhibiting Caspase-12 apoptosis pathway.

参考文献/References:

[1]Litviňuková M,Talavera-López C,Maatz H,et al.Cells of the adult human heart[J].Nature,2020,588(7838):466-472.[2]Ren J,Bi Y,Sowers JR,et al.Endoplasmic reticulum stress and unfolded protein response in cardiovascular diseases[J].Nat Rev Cardiol,2021,18(7):499-521.[3]Schirone L,Forte M,Palmerio S,et al.A Review of the Molecular Mechanisms Underlying the Development and Progression of Cardiac Remodeling[J].Oxid Med Cell Longev,2017,2017:3920195.[4]王添乐,杨晓,王剑.蛋白质稳态在病理性心脏重塑中的作用[J].中华心血管病杂志,2021,49(7):719-723.[5]Kosiborod M,Gomes MB,Nicolucci A,et al.Vascular complications in patients with type 2 diabetes:prevalence and associated factors in 38 countries (the DISCOVER study program)[J].Cardiovasc Diabetol,2018,17(1):150.[6]Cugusi L,Cadeddu C,Nocco S,et al.Effects of an Aquatic-Based Exercise Program to Improve Cardiometabolic Profile,Quality of Life,and Physical Activity Levels in Men With Type 2 Diabetes Mellitus[J].PMR,2015,7(2):141-148.[7]朱志中,张琳瑛,于洋.水疗法对老年慢性心力衰竭患者血流动力学和运动耐力的影响[J].中华老年医学杂志,2015,34(9):1003-1004.[8]Wang Y,Wang Q,Li C,et al.A Review of Chinese Herbal Medicine for the Treatment of Chronic Heart Failure[J].Curr Pharm Des,2017,23(34):5115-5124.[9]Varela R,Rauschert I,Romanelli G,et al.Hyperglycemia and hyperlipidemia can induce morphophysiological changes in rat cardiac cell line[J].Biochem Biophys Rep,2021,26:100983.[10]Tian JH,Wu Q,He YX,et al.Zonisamide,an antiepileptic drug,alleviates diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress[J].Acta pharmacol Sini,2021,42(3):393-403.[11]Schwarz DS,Blower MD.The endoplasmic reticulum:structure,function and response to cellular signaling[J].Cell Mol Life Sci,2016,73(1):79-94.[12]Ron D,Walter P.Signal integration in the endoplasmic reticulum unfolded protein response[J].Nat Rev Mol Cell Biol,2007,8(7):519-529.[13]Groenendyk J,Sreenivasaiah PK,Kim DH,et al.Biology of endoplasmic reticulum stress in the heart[J].Cir Res,2010,107(10):1185-1197.[14]Yang Q,Gao H,Dong R,et al.Sequential changes of endoplasmic reticulum stress and apoptosis in myocardial fibrosis of diabetes mellitus-induced rats[J].Mol Med Rep,2016,13(6):5037-5044.[15]单凤军.随意跑轮运动对糖尿病大鼠心肌NADPH氧化酶及心脏重塑的影响[J].中华物理医学与康复杂志,2020,42(11):967-972.[16]杨海玉,于涛,杨爱成,等.丹参酮ⅡA磺酸钠对2型糖尿病大鼠心肌TNF-αmRNA表达的影响[J].中国中医急症,2014,23(3):440-441,454.[17]上官若男,焦艺科,尚画雨,等.8周游泳运动对2型糖尿病大鼠心肌间质纤维化的影响[J].中国康复医学杂志,2018,33(2):158-164.[18]任磊,陈明慧,李平平,等.运动训练联合健心汤对心力衰竭大鼠GATA-4基因及蛋白表达的影响[J].中国中医急症,2020,29(5):841-845.[19]Chandrasekera DNK,Neale JPH,van Hout I,et al.Upregulation of microRNA-532 enhances cardiomyocyte apoptosis in the diabetic heart[J].Apoptosis,2020,25(5-6):388-399.[20]符显昭,冯雪萍,黄文华,等.降糖舒心方对糖尿病心肌内质网应激c-JNK凋亡旁路的影响[J].中华中医药学刊,2018,36(10):2423-2427.[21]邓爽,蒲锐,陈子扬,等.运动预适应干预压力超负荷心肌重构模型小鼠的心肌功能及细胞凋亡[J].中国组织工程研究,2022,26(5):717-723.[22]金娟,董晓阁,刘莉,等.参芪益心方抑制阿霉素诱导大鼠H9c2细胞凋亡的机制研究[J].中国中西医结合杂志,2021,41(6):729-735.[23]常盼,李静,张静,等.有氧运动对糖尿病小鼠心肌损伤及氧化应激和内质网应激的影响[J].山西医科大学学报,2021,52(3):264-269.[24]倪婳,李从圣,杨静,等.心力衰竭患者血清中内质网应激标志物GRP78的水平变化及其在评估近期预后中的价值[J].临床急诊杂志,2020,21(8):638-642.

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更新日期/Last Update: 1900-01-01