[1]张新颖.芪参益气滴丸含药血清抑制血管平滑肌细胞增殖作用的实验研究[J].医学信息,2022,35(11):130-133,137.[doi:10.3969/j.issn.1006-1959.2022.11.034]
　ZHANG Xin-ying.Experimental Study on Inhibition of Vascular Smooth Muscle Cell Proliferation by Serum Containing Qishen Yiqi Dropping Pill[J].Medical Information,2022,35(11):130-133,137.[doi:10.3969/j.issn.1006-1959.2022.11.034]
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芪参益气滴丸含药血清抑制血管平滑肌细胞增殖作用的实验研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年11期
页码:
130-133,137
栏目:
论著
出版日期:
2022-06-01

文章信息/Info

Title:
Experimental Study on Inhibition of Vascular Smooth Muscle Cell Proliferation by Serum Containing Qishen Yiqi Dropping Pill
文章编号:
1006-1959（2022）11-0130-05
作者:
张新颖
（天津市第四中心医院中医科，天津 300241）
Author(s):
ZHANG Xin-ying
(Department of Traditional Chinese Medicine,Tianjin Fourth Central Hospital,Tianjin 300241,China)
关键词:
芪参益气滴丸含药血清; 血管平滑肌; 细胞增殖; CCK8
Keywords:
Serum containing Qishen Yiqi dropping pill; Vascular smooth muscle; Cell proliferation; CCK8
分类号:
R322.1+2
DOI:
10.3969/j.issn.1006-1959.2022.11.034
文献标志码:
A
摘要:
目的 以芪参益气滴丸为例，探讨含药血清药理学作用，观察芪参益气滴丸含药血清对血管平滑肌的作用。方法 将雄性SD大鼠11只随机分为含药血清组6只，正常血清组5只。含药血清组给予芪参益气滴丸，按0.135 g/（kg·d）灌胃，正常血清组灌服等体积生理盐水，连续灌胃5 d，末次给药后提取含药血清。将含药血清分成：1%、3%、5%、10%、20%含药血清组，与相同浓度正常血清组同时作用于血管平滑肌，CCK8测定芪参益气滴丸含药血清对血管平滑肌细胞（VSMC）增殖的影响。结果 同步化较非同步化组细胞增殖稳定，误差较小；经24 h含药血清作用，5%含药血清、10%含药血清、20%含药血清均能抑制平滑肌细胞增殖，且10%含药血清抑制更明显，20%含药血清抑制相对较差；1%含药血清组与1%正常血清组不同时间点OD值比较，差异无统计学意义（P＞0.05）；作用于VSMC 6、12 h后，不同浓度含药血清组与正常血清组OD值比较，差异无统计学意义（P＞0.05）；作用于VSMC 24 h后，5%含药血清组OD值低于5%正常血清组（P＜0.01），10%含药血清组OD值低于10%正常血清组（P＜0.001）；作用于VSMC 48 h后，10%、20%含药血清组OD值低于正常血清组（P＜0.05）；5%含药血清OD值低于5%正常血清组（P＜0.001）；72 h后不同浓度梯度均出现较其他时间点下降趋势，3%、10%含药血清组OD值分别低于同浓度正常血清组（P＜0.05）；5%含药血清OD值低于5%正常血清组（P＜0.001）。结论 现代中药含药血清提取多样化，采用连续多次给药能有效保证含药血清的药效浓度。提取后血清经不同浓度实验，选取最佳浓度以利于进一步实验研究。
Abstract:
Objective To investigate the pharmacological effect of serum containing Qishen Yiqi Dropping Pills and observe the effect of serum containing Qishen Yiqi Dropping Pills on vascular smooth muscle.Methods Eleven male SD rats were randomly divided into drug-Containing serum group (6 rats) and normal serum group (5 rats). The drug-containing serum group was given Qishen Yiqi dropping pills by gavage at 0.135 g/(kg·d), and the normal serum group was given the same volume of normal saline by gavage for 5 days. After the last administration, the drug-containing serum was extracted. The drug-containing serum was divided into:1%, 3%, 5%, 10%, 20% drug-containing serum groups, and the same concentration of normal serum group at the same time on vascular smooth muscle cells, CCK8 determination of Qishen Yiqi dropping pills containing serum on VSMC proliferation.Results Compared with the non-synchronization group, the cell proliferation in the synchronization group was stable and the error was small;after 24-hour drug-containing serum treatment, 5% drug-containing serum, 10% drug-containing serum and 20% drug-containing serum groups could inhibit the proliferation of smooth muscle cells, and the inhibition of 10% drug-containing serum group was more obvious, while that of 20% drug-containing serum was relatively poor. There was no significant difference in OD value between 1% drug-containing serum group and 1% normal serum group at different time points (P>0.05). After acting on VSMC for 6 and 12 h, there was no significant difference in OD values between drug-containing serum groups with different concentrations and normal serum groups (P>0.05). After acting on VSMC for 24 h, the OD value of 5% drug-containing serum group was lower than that of 5% normal serum group (P<0.01), and that of 10% drug-containing serum group was lower than that of 10% normal serum group (P<0.001). After acting on VSMC for 48 h, OD values of 10% and 20% drug-containing serum groups were lower than those of normal serum groups (P<0.05). OD value of 5% drug-containing serum was lower than that of 5% normal serum group (P<0.001); after acting on VSMC for 72 h, the OD values at different concentrations showed a downward trend compared with those at other time points. The OD values of 3% and 10% drug-containing serum groups were lower than those of the normal serum group at the same concentration (P<0.05), and the OD value of 5% drug-containing serum was lower than that of 5% normal serum group (P<0.001).Conclusion The extraction of modern Chinese medicine drug-containing serum is diversified, and continuous multiple administration can effectively ensure the effective concentration of drug-containing serum; the serum after extraction was subjected to different concentrations, and the optimal concentration was selected for further experimental study.

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更新日期/Last Update: 1900-01-01