[1]何 跃,黄 丽,姚天玉,等.A20对脂多糖诱导人树突状细胞成熟度及功能的影响[J].医学信息,2023,36(01):86-89.[doi:10.3969/j.issn.1006-1959.2023.01.016]
 HE Yue,HUANG Li,YAO Tian-yu,et al.Effect of A20 on the Maturity and Function of Human Dendritic Cells Induced by Lipopolysaccharide[J].Journal of Medical Information,2023,36(01):86-89.[doi:10.3969/j.issn.1006-1959.2023.01.016]
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A20对脂多糖诱导人树突状细胞成熟度及功能的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年01期
页码:
86-89
栏目:
论著
出版日期:
2023-01-01

文章信息/Info

Title:
Effect of A20 on the Maturity and Function of Human Dendritic Cells Induced by Lipopolysaccharide
文章编号:
1006-1959(2023)01-0086-04
作者:
何 跃黄 丽姚天玉
(西南医科大学附属医院眼科1,风湿免疫科2,四川 泸州 646000)
Author(s):
HE YueHUANG LiYAO Tian-yuet al.
(Department of Ophthalmology1,Department of Rheumatology and Immunology2,the Affiliated Hospital ofSouthwest Medical University,Luzhou 646000,Sichuan,China)
关键词:
树突状细胞A20脂多糖成熟度细胞因子
Keywords:
Dendritic cellsA20LipopolysaccharidesMaturationCytokines
分类号:
R392.9
DOI:
10.3969/j.issn.1006-1959.2023.01.016
文献标志码:
A
摘要:
目的 探讨A20对脂多糖诱导的树突状细胞成熟度及功能的影响。方法 在脂多糖诱导树突状细胞成熟过程中,沉默A20,采用定量RT-PCR检测树突状细胞中A20的表达水平,流式细胞术检测树突状细胞表面共刺激分子CD80、CD86及HLA-DR的表达水平,ELISA检测树突状细胞分泌的细胞因子IL-1β、IL-6和IL-27的水平。结果 荧光显微镜下观察到绝大部分DCs被携带有GFP荧光基团标记的腺病毒转染,呈绿色荧光表达;定量RT-PCR检测显示,转染A20-shRNA腺病毒的树突状细胞A20的表达低于未转染腺病毒的树突状细胞(P<0.05);流式细胞术检测显示,沉默A20后,树突状细胞细胞表面的成熟标记分子CD80、CD86和HLA-DR的表达水平没有变化;ELISA检测显示,沉默A20的树突状细胞分泌IL-1β和IL-6的水平增加(P<0.05),而IL-27的水平降低(P<0.05)。结论 携带A20-shRNA的腺病毒能够顺利转染树突状细胞,且A20对树突状细胞的成熟度无调控作用,但沉默A20后能够促进树突状细胞分泌促炎细胞因子IL-1β和IL-6,降低抑炎细胞因子IL-27的表达水平。
Abstract:
Objective To investigate the effect of A20 on the maturity and function of human dendritic cells induced by lipopolysaccharide.Methods During the maturation of dendritic cells induced by lipopolysaccharide, A20 was silenced. The expression level of A20 in dendritic cells was detected by quantitative RT-PCR. The expression levels of costimulatory molecules CD80, CD86 and HLA-DR on dendritic cells were detected by flow cytometry. The levels of cytokines IL-1β, IL-6 and IL-27 secreted by dendritic cells were detected by ELISA.Results Under the fluorescence microscope, most of the DCs were transfected with adenovirus carrying GFP fluorescent group, showing green fluorescence expression. Quantitative RT-PCR showed that the expression of A20 in dendritic cells transfected with A20-shRNA adenovirus was lower than that in dendritic cells not transfected with adenovirus (P<0.05). Flow cytometry showed that the expression levels of mature marker molecules CD80, CD86 and HLA-DR on the surface of dendritic cells did not change after silencing A20. ELISA showed that the levels of IL-1β and IL-6 secreted by A20 dendritic cells increased (P<0.05), while the level of IL-27 decreased (P<0.05).Conclusion Adenovirus carrying A20-shRNA can successfully transfect dendritic cells, and A20 has no regulatory effect on the maturity of dendritic cells. However, silencing A20 can promote the secretion of pro-inflammatory cytokines IL-1β and IL-6 by dendritic cells and reduce the expression of anti-inflammatory cytokine IL-27.

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更新日期/Last Update: 1900-01-01