[1]石春蓉,余良主.基于GEO数据库分析心脏重塑中的焦亡相关核心基因及潜在治疗药物[J].医学信息,2024,37(05):12-19.[doi:10.3969/j.issn.1006-1959.2024.05.002]
　SHI Chun-rong,YU Liang-zhu.Identification of Pyroptosis-related Hub Genes and Potential Therapeutic Drugs in Cardiac Remodeling Based on GEO Database[J].Journal of Medical Information,2024,37(05):12-19.[doi:10.3969/j.issn.1006-1959.2024.05.002]
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基于GEO数据库分析心脏重塑中的焦亡相关核心基因及潜在治疗药物()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年05期
页码:
12-19
栏目:
生物信息学
出版日期:
2024-03-01

文章信息/Info

Title:
Identification of Pyroptosis-related Hub Genes and Potential Therapeutic Drugs in Cardiac Remodeling Based on GEO Database
文章编号:
1006-1959（2024）05-0012-08
作者:
石春蓉; 余良主
（湖北科技学院图书馆1，医学部基础医学院2，湖北 咸宁 437100）
Author(s):
SHI Chun-rong; YU Liang-zhu
(Library1,School of Basic Medical Sciences2,Hubei University of Science and Technology,Xianning 437100,Hubei,China)
关键词:
细胞焦亡; 心脏重塑; 核心基因; 尼美舒利
Keywords:
Cell pyroptosis; Cardiac remodeling; Hub genes; Nimesulli
分类号:
R541
DOI:
10.3969/j.issn.1006-1959.2024.05.002
文献标志码:
A
摘要:
目的 从细胞焦亡角度利用生物信息学方法挖掘心脏重塑相关的核心基因，分析其功能，预测可能的治疗药物，为心脏重塑的诊断与治疗提供理论依据。方法 从美国国立医学图书馆基因表达数据库GEO中下载心脏重塑相关基因芯片数据集，利用GEO2R在线分析工具鉴定心脏重塑相关差异表达基因（DEGs）。在GeneCards数据库收集细胞焦亡相关基因，将细胞焦亡相关基因与上述DEGs的交集基因构建蛋白互作网络，鉴定其中的核心基因。利用Metascape2.0对交集基因进行GO及KEGG功能富集分析。最后利用DGIdb数据库预测潜在药物与上述核心基因的相互作用。结果 在GEO数据库筛选出GSE24489数据集，利用GEO2R分析工具鉴定出1767个心脏重塑相关DEGs，与GeneCards数据库中的细胞焦亡相关基因进行交联，得到45个细胞焦亡相关的心脏重塑DEGs。这些DEGs的GO分析生物过程主要富集在防御反应的调节、蛋白裂解的调节、炎症反应等。KEGG通路富集分析显示细胞焦亡相关DEGs主要富集于阿尔茨海默病、癌症通路、丙型肝炎、PI3K-Akt信号途径等。从45个DEGs中鉴定出7个心脏重塑相关核心基因（TLR3、PTGS2、CASP1、CASP8、GSK3β、VEGFA、MKI67）。DGIdb数据库预测潜在药物与核心基因相互作用的结果显示，尼美舒利（Nimesulide）、塞来昔布（Celecoxib）、GSK3β抑制剂Tideglusib等药物可能成为治疗心脏重塑的关键候选药物。结论 核心基因PTGS2等在心脏重塑的发生发展中具有重要作用，靶向调节这些核心基因可抑制心脏重塑的发生。
Abstract:
Objective To explore the the pyroptosis-related hub genes in cardiac remodeling by bioinformatics methods from the perspective of pyroptosis, analyze their functions, predict possible therapeutic drugs, and provide theoretical basis for the diagnosis and treatment of cardiac remodeling.Methods The gene chip data set related to cardiac remodeling was downloaded from the Gene Expression Omnibus (GEO) database of the National Library of Medicine in the United States, and the differentially expressed genes (DEGs) related to cardiac remodeling were identified by GEO2R online analysis tool. The pyroptosis-related genes were collected in the GeneCards database, and the protein interaction network was constructed between the pyroptosis-related genes and the intersection genes of the above DEGs to identify the core genes. Metascape2.0 was used to perform GO and KEGG functional enrichment analysis on the intersection genes. Finally, the DGIdb database was used to predict the interaction between potential drugs and the above core genes.Results The dataset GSE24489 was screened out from the GEO database as a cardiac remodeling-related dataset, and 1767 cardiac remodeling-related DEGs were screened out from GSE24489 and intersected with the pyroptosis-related genes from the GeneCards database to obtain 45 pyroptosis-related DEGs in cardiac remodeling. GO analysis of these DEGs showed that biological processes were mainly enriched in the regulation of defense response, regulation of protein cleavage, inflammatory response, etc. KEGG pathway enrichment analysis showed that DEGs related to pyroptosis were mainly enriched in Alzheimer’s disease, cancer pathway, hepatitis C, PI3K-Akt signaling pathway and so on. Nine cardiac remodeling-related hub DEGs (TLR3、PTGS2、CASP1、CASP8、GSK3β、VEGFA、MKI67) were identified from these pyroptosis-related DEGs. The results of the DGIdb database predicting the interaction between potential drugs and hub genes showed that drugs such as Nimesulide, Celecoxib, and GSK3β inhibitor Tideglusib might become key candidates for the treatment of cardiac remodeling.Conclusion These identified hub genes such as PTGS2 may play an important role in the occurrence and development of cardiac remodeling and represent new therapeutic targets for the treatment of heart remodeling.

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更新日期/Last Update: 1900-01-01