[1]刘树义,兰 凯,张旭东,等.自噬对激素性股骨头坏死大鼠模型血脂及血液流变学的影响[J].医学信息,2022,35(03):8-12.[doi:10.3969/j.issn.1006-1959.2022.03.002]
　LIU Shu-yi,LAN Kai,ZHANG Xu-dong,et al.Effect of Autophagy on Blood Lipid and Hemorheology in Rat Model withSteroid-induced Osteonecrosis of the Femoral Head[J].Medical Information,2022,35(03):8-12.[doi:10.3969/j.issn.1006-1959.2022.03.002]
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自噬对激素性股骨头坏死大鼠模型血脂及血液流变学的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年03期
页码:
8-12
栏目:
出版日期:
2022-02-01

文章信息/Info

Title:
Effect of Autophagy on Blood Lipid and Hemorheology in Rat Model withSteroid-induced Osteonecrosis of the Femoral Head
文章编号:
1006-1959（2022）03-0008-05
作者:
刘树义1; 兰 凯1; 张旭东1; 李 瑜2
1.西安医学院临床医学院外科学教研室，陕西 西安 710021； 2.西安医学院第二附属医院耳鼻咽喉科，陕西 西安 710038
Author(s):
LIU Shu-yi1; LAN Kai1; ZHANG Xu-dong1; LI Yu2
1.The Teaching and Research Section of Surgery,School of Clinical Medical,Xi’an Medical College,Xi’an 710021,Shaanxi,China;2.Department of Otolaryngology,the Second Affiliated Hospital of Xi’an Medical College,Xi’an 710038,Shaanxi,China
关键词:
自噬; 激素性股骨头坏死; 血脂; 血液流变学
Keywords:
Autophagy; Hormone-induced femoral head necrosis; Blood lipid; Hemorheology
分类号:
R459.1；R274.9
DOI:
10.3969/j.issn.1006-1959.2022.03.002
文献标志码:
A
摘要:
目的 观察自噬活性对激素性股骨头坏死大鼠血脂及血液流变学的影响，探讨其潜在的治疗途径。方法 选择24只无特定病原体（SPF级）SD大鼠（雌鼠12只、雄鼠12只），采用随机抽样法分为对照组、模型组、自噬阻断组及自噬激动组4组，每组雌、雄各3只。对照组给予生理盐水，模型组、自噬阻断组、自噬激动组分别给予甲强龙、甲强龙+3-甲基腺嘌呤（3-MA）、甲强龙+雷帕霉素（rapamycin）。模型组、自噬阻断组及自噬激动组大鼠腹腔注射脂多糖（20 μg/kg）2次，每次间隔24 h，24 h后两侧臀肌交替注射甲强龙（40 mg/kg）3次，每次间隔24 h。自噬阻断组与自噬激动组每次肌肉注射甲强龙前30 min，经腹腔分别注射3-MA（2.5 mg/kg）、雷帕霉素（1 mg/kg）。对照组腹腔及臀肌注射等量生理盐水。继续饲养8周后经腹主动脉采血，测定血液流变学和血脂含量。结果 对照组、模型组、自噬阻断组及自噬激动组股骨头坏死率分别为0、83.33%、16.67%、100.00%，组间比较，差异有统计学意义（P＜0.05）；使用激素后模型组甘油三酯（TG）、总胆固醇（TCH）及高密度脂蛋白（HDL）均高于正对照组和自噬阻断组，自噬激动组上述三项指标高于模型组，差异有统计学意义（P＜0.05）；四组低密度脂蛋白（LDL）比较，差异无统计学意义（P＞0.05）；模型组全血黏度、血浆黏度、红细胞压积及红细胞聚集指数均高于对照组和自噬阻断组，自噬激动组上述血液流变学指标高于其他三组，差异有统计学意义（P＜0.05）。结论 诱导自噬不仅能够改善因激素导致的血脂异常增高状态，同时也能改善异常的血液流变状态，对大鼠激素性股骨头坏死可能具有治疗作用。
Abstract:
Objective To observe the effect of autophagy activity on blood lipid and hemorheology in rats with steroid-induced femoral head necrosis, and to explore its potential therapeutic approach.Methods Twenty-four SPF SD rats (12 females and 12 males) were randomly divided into control group, model group, autophagy blocking group and autophagy activation group, with 3 males and 3 females in each group. The control group was given normal saline. The model group, autophagy blocking group and autophagy activation group were given methylprednisolone, methylprednisolone+3-methyladenine (3-MA) and methylprednisolone+rapamycin, respectively. Rats in model group, autophagy block group and autophagy activation group were given intraperitoneal injection of lipopolysaccharide (20 μg/kg) twice at an interval of 24 h. After 24 h, methylprednisolone (40 mg/kg) was alternately injected into bilateral gluteus muscles for 3 times at an interval of 24 h. The autophagy blocking group and the autophagy activation group were injected with methylprednisolone 30 min before each intramuscular injection, and 3-MA (2.5 mg/kg) and rapamycin (1 mg/kg) were injected intraperitoneally. The control group was injected with the same amount of normal saline in abdominal cavity and gluteal muscle. After 8 weeks of continuous feeding, blood samples were collected from abdominal aorta to determine blood rheology and lipid content.Results The femoral head necrosis rate of the control group, the model group, the autophagy blocking group and the autophagy activation group were 0, 83.33%, 16.67% and 100.00%, respectively, and the difference was statistically significant (P<0.05). The levels of triglyceride (TG), total cholesterol (TCH) and high density lipoprotein (HDL) in the model group were higher than those in the positive control group and the autophagy blocking group, the above three indexes in the autophagy activation group were higher than those in the model group, and the difference was statistically significant (P<0.05); there was no significant difference in low density lipoprotein (LDL) among the four groups (P>0.05). The whole blood viscosity, plasma viscosity, hematocrit and erythrocyte aggregation index in the model group were higher than those in the control group and the autophagy blocking group, and the above hemorheology indexes in the autophagy activation group were higher than those in the other three groups, the difference was statistically significant (P<0.05).Conclusion Induction of autophagy can not only improve the abnormal increase of blood lipid caused by hormones, but also improve the abnormal hemorheology, which may have therapeutic effect on steroid-induced femoral head necrosis in rats.

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更新日期/Last Update: 1900-01-01