[1]彭 瑶,邓丹萍,容 婷,等.ALOX15对BGC823细胞铁死亡的作用研究[J].医学信息,2024,37(07):69-72,88.[doi:10.3969/j.issn.1006-1959.2024.07.012]
　PENG Yao,DENG Dan-ping,RONG Ting,et al.Effect of ALOX15 on Ferroptosis in BGC823 Cells[J].Journal of Medical Information,2024,37(07):69-72,88.[doi:10.3969/j.issn.1006-1959.2024.07.012]
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ALOX15对BGC823细胞铁死亡的作用研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年07期
页码:
69-72,88
栏目:
论著
出版日期:
2024-04-01

文章信息/Info

Title:
Effect of ALOX15 on Ferroptosis in BGC823 Cells
文章编号:
1006-1959（2024）07-0069-05
作者:
彭 瑶; 邓丹萍; 容 婷; 等
（1.湖南中医药大学第二附属医院急诊科，湖南 长沙 410000；2.郴州市中医院医务科，湖南 郴州 423000；3.湖南中医药大学研究生院，湖南 长沙 410208）
Author(s):
PENG Yao; DENG Dan-ping; RONG Ting; et al.
(1.Department of Emergency,the Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410000,Hunan,China;2.Department of Medical,Chenzhou Hospital of Traditional Chinese Medicine,Chenzhou 423000,Hunan,China;3.Graduate School,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China)
关键词:
铁死亡; 胃癌; ALOX15; BGC823细胞
Keywords:
Ferroptosis; Gastric cancer; ALOX15; BGC823 cells
分类号:
R735.2
DOI:
10.3969/j.issn.1006-1959.2024.07.012
文献标志码:
A
摘要:
目的 探讨ALOX15对BGC823细胞铁死亡的作用。方法 建立过表达ALOX15或干扰ALOX15表达的BGC823细胞株，采用实时荧光定量PCR法和蛋白免疫印迹（Western Blot）法验证其转染效率。CCK-8法检测过表达ALOX15或干扰ALOX15表达的BGC823细胞的增殖情况，使用试剂盒测定各组细胞中亚铁离子（Fe2+）、活性氧（ROS）、丙二醛（MDA）、谷胱甘肽（GSH）水平，Western Blot法检测各组细胞中谷胱甘肽过氧化物酶4（GPX4）蛋白水平。结果 成功构建过表达ALOX15或干扰ALOX15表达的BGC823细胞株。pcDNA3.1-ALOX15组24、48 h细胞增殖率及GSH水平、GPX4表达水平低于pcDNA3.1组、Control组，ROS水平、MDA水平、Fe2+水平、ALOX15 mRNA及蛋白水平高于pcDNA3.1组、Control组，差异有统计学意义（P＜0.05）；而转染si-ALOX15后，si-ALOX15组BGC823细胞24、48 h细胞增殖率及GSH、GPX4表达水平高于si-NC组，ROS水平、MDA水平、Fe2+水平、ALOX15 mRNA及蛋白水平低于si-NC组，差异有统计学意义（P＜0.05）。结论 上调ALOX15的蛋白表达水平可促进胃癌细胞铁死亡。
Abstract:
Objective To investigate the effect of ALOX15 on ferroptosis in BGC823 cells.Methods BGC823 cell lines with ALOX15 overexpression or ALOX15 interference were established, and the transfection efficiency was verified by quantitative real-time PCR and Western Blot. The proliferation of BGC823 cells overexpressing ALOX15 or interfering with ALOX15 expression was detected by CCK-8 method. The levels of ferrous ion (Fe2+), reactive oxygen species (ROS), malondialdehyde (MDA) and glutathione (GSH) in each group were determined by using kits. The protein level of glutathione peroxidase 4(GPX4)in each group was detected by Western Blot.Results BGC823 cell lines overexpressing ALOX15 or interfering with ALOX15 expression were successfully constructed. The cell proliferation rate at 24, 48 h, GSH level and GPX4 expression level of pcDNA3.1-ALOX15 group were lower than those of pcDNA3.1 group and Control group, while ROS level, MDA level, Fe2+ level, ALOX15 mRNA and protein level were higher than those of pcDNA3.1 group and Control group, and the differences were statistically significant (P<0.05). After transfection of si-ALOX15, the cell proliferation rate at 24,48 h and the expression of GSH level and GPX4 level in BGC823 cells in si-ALOX15 group were higher than those in si-NC group, and ROS level, MDA level, Fe2+ level, ALOX15 mRNA and protein were lower than those in si-NC group, the differences were statistically significant (P<0.05).Conclusion Up-regulation of ALOX15 protein expression promotes ferroptosis in gastric cancer cells.

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更新日期/Last Update: 1900-01-01