[1]钟纯洁.脑缺血/再灌注损伤早期大鼠脑组织HO-1/HO-1mRNA表达趋势[J].医学信息,2018,31(19):8-11.[doi:10.3969/j.issn.1006-1959.2018.19.003]
　ZHONG Chun-jie.Trend of HO-1/HO-1mRNA Expression in Rat Brain Tissue during the Early Stage of Cerebral Ischemia /Reperfusion Injury[J].Medical Information,2018,31(19):8-11.[doi:10.3969/j.issn.1006-1959.2018.19.003]
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脑缺血/再灌注损伤早期大鼠脑组织HO-1/HO-1mRNA表达趋势()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年19期
页码:
8-11
栏目:
出版日期:
2018-10-01

文章信息/Info

Title:
Trend of HO-1/HO-1mRNA Expression in Rat Brain Tissue during the Early Stage of Cerebral Ischemia /Reperfusion Injury
文章编号:
1006-1959（2018）19-0008-04
作者:
钟纯洁
天津市宝坻区人民医院感染科，天津 301800
Author(s):
ZHONG Chun-jie
Department of Infectious Diseases,Baodi District People’s Hospital,Tianjin 301800,China
关键词:
脑缺血/再灌注损伤; HO-1; HO-1mRNA
Keywords:
Cerebral ischemia/reperfusion injury; HO-1; HO-1 mRNA
分类号:
R743.3
DOI:
10.3969/j.issn.1006-1959.2018.19.003
文献标志码:
A
摘要:
目的 观察在脑缺血/再灌注损伤大鼠脑组织中不同时间点HO-1/HO-1mRNA的表达趋势，为脑缺血/再灌注损伤的机制研究与防治提供了一定的理论依据。方法 成年雄性SD大鼠25只随机分为五组：假手术组，脑缺血2 h再灌注3 h组，6 h组，12 h组和24 h组，每组5只。各组大鼠分别于再灌注后相应时间点取缺血侧大脑皮质进行RT-qPCR检测，对HO-1基因mRNA的表达进行定量分析；采用Western blotting方法检测各时间点HO-1蛋白表达。结果 假手术组HO-1基因mRNA表达水平较低，与之相比脑缺血/再灌注3 h时升高（P＜0.05），再灌注6 h达到高峰（P＜0.05），与6 h相比再灌注12 h时降低（P＜0.05），与再灌注24 h接近但仍高于再灌注3 h时水平（P＜0.05）；HO-1蛋白在缺血再灌注各个时间点表达规律与HO-1基因mRNA表达水平一致。结论 脑缺血/再灌注损伤后大鼠缺血脑组织中HO-1/HO-1mRNA表达水平于损伤早期（6 h）迅速升高，达高峰后又逐渐下降并保持在一定水平，是脑缺血/再灌注损伤早期重要机制之一。
Abstract:
Objective To observe the expression of HO-1/HO-1 mRNA at different time points in rats with cerebral ischemia/reperfusion injury,and provide a theoretical basis for the study and prevention of cerebral ischemia/reperfusion injury.Methods 25 adult male Sprague-Dawley rats were randomly divided into five groups:sham operation group,cerebral ischemia 2 h reperfusion 3 h group,6 h group,12 h group and 24 h group,5 rats in each group.Rats in each group were examined by RT-qPCR at the corresponding time points after reperfusion.The expression of HO-1 gene mRNA was quantitatively analyzed.Western blotting was used to detect HO-1 protein expression at each time point.Results The expression level of HO-1 gene mRNA was lower in sham operation group.In contrast,cerebral ischemia/reperfusion increased at 3 h(P<0.05),reached the peak at 6 h(P<0.05),decreased at 12 h after reperfusion compared with 6 h(P<0.05),and was close to 24 h after reperfusion,but still higher than that at 3 h after reperfusion(P<0.05).The expression of HO-1 protein was consistent with the expression of HO-1 gene mRNA at different time points after ischemia reperfusion.Conclusion The expression of HO-1/HO-1mRNA in the ischemic brain tissue of rats after cerebral ischemia/reperfusion injury increased rapidly at the early stage of injury(6 h),then decreased gradually and maintained at a certain level after reaching the peak,which is one of the important mechanisms in the early stage of cerebral ischemia /reperfusion injury.

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更新日期/Last Update: 2018-10-29