[1]冯水土,冯丽华,任 芳,等.PPZ与5-FU对胃癌细胞生长、自我更新能力的影响及相互作用[J].医学信息,2019,32(21):61-65.[doi:10.3969/j.issn.1006-1959.2019.21.020]
　FENG Shui-tu,FENG Li-hua,REN Fang,et al.Effects of PPZ and 5-FU on the Growth and Self-Renewal Ability of Gastric Cancer Cells and their Interaction[J].Journal of Medical Information,2019,32(21):61-65.[doi:10.3969/j.issn.1006-1959.2019.21.020]
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PPZ与5-FU对胃癌细胞生长、自我更新能力的影响及相互作用()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年21期
页码:
61-65
栏目:
论著
出版日期:
2019-11-01

文章信息/Info

Title:
Effects of PPZ and 5-FU on the Growth and Self-Renewal Ability of Gastric Cancer Cells and their Interaction
文章编号:
1006-1959（2019）21-0061-05
作者:
冯水土; 冯丽华; 任 芳; 林雨标; 李 翎; 郑文慧; 陈毅德
（厦门市海沧医院肿瘤内科，福建 厦门 361026）
Author(s):
FENG Shui-tu; FENG Li-hua; REN Fang; LIN Yu-biao; LI Ling; ZHENG Wen-hui; CHEN Yi-de
(Department of Oncology,Haicang Hospital,Xiamen 361026,Fujian,China)
关键词:
胃癌; PPIs; 5-FU; 肿瘤成球能力; 自我更新
Keywords:
Gastric cancer; PPIs; 5-FU; Tumor globular ability; Self-renewal
分类号:
R735.2
DOI:
10.3969/j.issn.1006-1959.2019.21.020
文献标志码:
A
摘要:
目的 探讨泮托拉唑（PPZ）、5-氟尿嘧啶（5-FU）在调控胃癌细胞及胃癌干细胞生长、自我更新能力中的影响及其相互作用。方法 将SGC-7901和HGC-27细胞分为3组实验：5-Fu处理组、PPZ处理组和5-Fu+PPZ组。通过细胞成球实验检测PPZ加药前后胃癌细胞系（SGC7901、HGC-27）中胃癌细胞及胃癌干细胞自我更新能力的变化，观察PPZ对胃癌细胞系成球能力干扰情况；MTT法检测PPZ、5-FU对胃癌细胞及胃癌干细胞增殖能力的影响，观察PPZ对5-FU药物敏感性的调节作用。结果 PPZ加入后胃癌细胞系（SGC7901、HGC-27）和胃癌干细胞系（SGC7901-SP、 HGC-27-SP）自我更新率比PPZ加入前的自我更新率下降（P＜0.01）；PPZ、5-FU对胃癌细胞（SGC7901、HGC-27）增殖均有抑制作用，而5-Fu+PPZ联合组抑制最为明显，抑制增殖的作用在24 h开始出现，96 h最低，均低于0 h。PPZ对胃癌干细胞（SGC7901-SP、HGC-27-SP）增殖均有抑制作用，在48 h逐渐明显；加入PPZ后，胃癌干细胞（SGC7901-SP、HGC-27-SP）两个细胞系酶标仪检测到的吸光值在48 h、72 h、96 h时均下降。72 h和96 h时，加与不加PPZ的吸光值比较，统计学意义显著（P＜0.01）。不同浓度（0~50 μg/ml）5-FU对胃癌干细胞（SGC7901-SP、HGC-27-SP）增殖抑制的差异不显著；而加入PPZ 100 μg/ml后，随着5-FU浓度的增加增殖抑制作用逐渐增强，在40~50 μg/ml浓度的5-FU对胃癌干细胞（SGC7901-SP、HGC-27-SP）增殖抑制更加明显；加入PPZ 后，40 μg/ml及50 μg/ml浓度的5-FU作用下胃癌干细胞（SGC7901-SP、HGC-27-SP）酶标仪检测到的吸光值均降低。结论 PPZ能有效抑制胃癌细胞及胃癌干细胞的自我更新能力，抑制其增殖，并可提高其对5-FU的化疗敏感性。PPZ有望成为逆转胃癌耐药的一类联合治疗药物应用于临床。
Abstract:
Objective To investigate the effects of pantoprazole (PPZ) and 5-fluorouracil (5-FU) on the growth and self-renewal of gastric cancer cells and gastric cancer stem cells. Methods SGC-7901 and HGC-27 cells were divided into three groups of experiments: 5-Fu treatment group, PPZ treatment group and 5-Fu combined with PPZ treatment group. The changes of self-renewal ability of gastric cancer cells and gastric cancer stem cells in gastric cancer cell lines (SGC7901, HGC-27) before and after PPZ dosing were detected by cell globule assay. The interference of PPZ on the ability of gastric cancer cell line to form a ball was observed. MTT assay for PPZ, 5-FU effect on the proliferation of gastric cancer cells and gastric cancer stem cells, and to observe the regulation of PPZ on its 5-FU drug sensitivity.Results The self-renewal rate of gastric cancer cell lines (SGC7901, HGC-27) and gastric cancer stem cell lines (SGC7901-SP, HGC-27-SP) after PPZ addition was lower than that before PPZ(P<0.01). PPZ and 5-FU inhibited the proliferation of gastric cancer cells (SGC7901, HGC-27), while the 5-Fu+PPZ combination showed the most obvious inhibition. The inhibition of proliferation began to appear at 24 h, the lowest at 96 h,Both are below 0 h. PPZ inhibited the proliferation of gastric cancer stem cells (SGC7901-SP, HGC-27-SP), and became obvious at 48 h. After adding PPZ, two cell lines of gastric cancer stem cells (SGC7901-SP, HGC-27-SP) were labeled. The absorbance values detected by the instrument decreased at 48 h, 72 h, and 96 h. At 72 h and 96 h, the absorbance values of the plus and without PPZ were statistically significant (P<0.01). Different concentrations (0~50 μg/ml) of 5-FU had no significant difference in the inhibition of proliferation of gastric cancer stem cells (SGC7901-SP, HGC-27-SP), but increased with the concentration of 5-FU after adding PPZ 100μg/ml. The inhibition of proliferation was gradually enhanced, and the proliferation of gastric cancer stem cells (SGC7901-SP, HGC-27-SP) was more obvious in 5-FU (40~50 μg/ml) concentration; 40 μg/ml and 50 μg were added after adding PPZ. At the concentration of 5-FU, the absorbance values of gastric cancer stem cells (SGC7901-SP, HGC-27-SP) were decreased.Conclusion PPZ can effectively inhibit the self-renewal ability of gastric cancer cells and gastric cancer stem cells, inhibit their proliferation, and improve their chemosensitivity to 5-FU. PPZ is expected to be a combination of a combination of therapeutic drugs for reversing gastric cancer.

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更新日期/Last Update: 2019-11-01