[1]王玲玲,吴宗丙,罗继敏,等.HR-HPV载量和HR-HPV E6或E7 mRNA在宫颈癌和癌前病变的表达和意义[J].医学信息,2022,35(08):97-100.[doi:10.3969/j.issn.1006-1959.2022.08.023]
　WANG Ling-ling,WU Zong-bing,LUO Ji-min,et al.Expression and Significance of HR-HPV Load and HR-HPV E6 or E7 mRNA in Cervical Cancer and Precancerous Lesions[J].Medical Information,2022,35(08):97-100.[doi:10.3969/j.issn.1006-1959.2022.08.023]
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HR-HPV载量和HR-HPV E6或E7 mRNA在宫颈癌和癌前病变的表达和意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年08期
页码:
97-100
栏目:
论著
出版日期:
2022-04-15

文章信息/Info

Title:
Expression and Significance of HR-HPV Load and HR-HPV E6 or E7 mRNA in Cervical Cancer and Precancerous Lesions
文章编号:
1006-1959（2022）08-0097-04
作者:
王玲玲; 吴宗丙; 罗继敏; 等
（1.黔南州人民医院病理科，贵州 黔南 550008；2.黔南州中医院病理科，贵州 黔南 550008）
Author(s):
WANG Ling-ling; WU Zong-bing; LUO Ji-min; et al.
(1.Department of Pathology,the People’s Hospital of Qiannan,Qiannan 550008,Guizhou,China;2.Department of Pathology,Qiannan Hospital of Traditional Chinese Medicine,Qiannan 550008,Guizhou,China)
关键词:
HR-HPV; mRNA; 宫颈癌; 癌前病变
Keywords:
HR-HPV; mRNA; Cervical cancer; Precancerous lesion
分类号:
R737.33
DOI:
10.3969/j.issn.1006-1959.2022.08.023
文献标志码:
A
摘要:
目的 观察高危型人乳头瘤病毒（HR-HPV）载量和HR-HPV E6与E7 mRNA在宫颈癌和癌前病变的表达情况和意义。方法 选取2020年5月-2021年5月在我院诊治的89例HPV持续感染患者为研究对象，依据病理组织分为宫颈癌组（n=33）、宫颈上皮内瘤变（CIN）Ⅰ组（n=19）、CINⅡ组（n=17）、CINⅢ组（n=20），分别行HR-HPV载量、HR-HPV E6或E7 mRNA检测，比较各组HR-HPV DNA载量、HR-HPV E6或E7 mRNA检出阳性率和表达水平，分析不同HR-HPV载量各类型宫颈病变的发生率以及不同宫颈病变与HR-HPV载量、HR-HPV E6或E7 mRNA的相关性。结果 各组HR-HPV DNA阳性率均大于阴性率，宫颈癌组、CINⅡ组、CINⅢ组HR-HPV E6或E7 mRNA阳性率均大于阴性率，CINⅠ组阳性率小于阴性率，差异有统计学意义（P＜0.05）；CINⅡ组HR-HPV DNA载量阳性率与HR-HPV E6或E7 mRNA比较，差异无统计学意义（P＞0.05）；宫颈癌组、CINⅢ组HR-HPV DNA阳性率、HR-HPV E6或E7 mRNA阳性率均大于CINⅠ组、CINⅡ组，差异有统计学意义（P＜0.05），宫颈癌组与CINⅢ组比较，差异无统计学意义（P＞0.05），但CINⅡ组大于CINⅠ组，差异有统计学意义（P＜0.05）；宫颈癌组、CINⅡ组、CINⅢ组HR-HPV DNA载量、HR-HPV E6或E7 mRNA水平均高于CINⅠ组，差异有统计学意义（P＜0.05），宫颈癌组与CIN Ⅲ组比较，差异无统计学意义（P＞0.05）；CINⅠ组HR-HPV DNA载量水平与CINⅡ比较，差异无统计学意义（P＞0.05），CINⅡ组HR-HPV E6或E7 mRNA水平高于CINⅠ组，差异有统计学意义（P＜0.05）；不同HR-HPV载量组，CINⅠ、CINⅡ、CINⅢ和宫颈癌的发生率比较，差异有统计学意义（P＜0.05）；HPV E6或E7 mRNA和HR-HPV DNA间存在相关性，CINⅠ、CINⅡ与其呈正相关（P＜0.05），CINⅢ与宫颈癌无相关性（P＞0.05）。结论 在宫颈上皮内瘤变CINⅠ阶段，HR-HPV和HR-HPV E6或E7 mRNA均开始表达，且随表达量升高，病理分级升高，进一步加速宫颈癌的发生。同时两者存在相关性，揭示了HPV载量与病变严重程度的一致性。
Abstract:
Objective To observe the expression and significance of the high risk human papillomavirus (HR-HPV) load and the expression of HR-HPV E6 and E7 mRNA in cervical cancer and precancerous lesions.Methods A total of 89 patients with persistent HPV infection diagnosed and treated in our hospital from May 2020 to May 2021 were selected and divided into cervical cancer group (n=33), cervical intraepithelial neoplasia (CIN)Ⅰ group (n=19), CINⅡ group (n=17), and CINⅢ group (n=20) according to the pathological tissue. HR-HPV load, HR-HPV E6 or E7 mRNA were detected respectively, and the positive rates and expression levels of HR-HPV DNA load, HR-HPV E6 or E7 mRNA in each group were compared. The incidence of various types of cervical lesions with different HR-HPV loads and the correlation between different cervical lesions and HR-HPV loads, HR-HPV E6 or E7 mRNA were analyzed.Results The positive rate of HR-HPV DNA in each group was higher than the negative rate, the positive rate of HR-HPV E6 or E7 mRNA in cervical cancer group, CINⅡ group and CINⅢ group was higher than the negative rate, the difference was statistically significant (P<0.05). There was no significant difference in the positive rate of HR-HPV DNA load between CINⅡ group and HR-HPV E6 or E7 mRNA (P>0.05); the positive rates of HR-HPV DNA and HR-HPV E6 or E7 mRNA in cervical cancer group and CINⅢ group were higher than those in CINⅠ group and CINⅡ group, and the difference was statistically significant (P<0.05), there was no significant difference between cervical cancer group and CINⅢ group (P>0.05), but the CINⅡ group was greater than CINⅠ group, and the difference was statistically significant (P<0.05). The HR-HPV DNA load, HR-HPV E6 or E7 mRNA levels in cervical cancer group, CINⅡ group and CINⅢ group were higher than those in CINⅠ group, and the differences were statistically significant (P<0.05); there was no significant difference between cervical cancer group and CINⅢ group (P>0.05); there was no significant difference in HR-HPV DNA load between CINⅠ group and CINⅡ group (P>0.05); the mRNA levels of HR-HPV E6 or E7 in CINⅡ group were higher than those in CINⅠ group, and the difference was statistically significant (P<0.05). There was significant difference in the incidence of CINⅠ, CINⅡ, CINⅢ and cervical cancer among different HR-HPV loading groups (P<0.05). There was a correlation between HPV E6 or E7 mRNA and HR-HPV DNA, and CINⅠ and CINⅡ were positively correlated (P<0.05), there was no correlation between CINⅢ and cervical cancer (P>0.05).Conclusion In the CIN Ⅰ stage of cervical intraepithelial neoplasia, HR-HPV and HR-HPV E6 or E7 mRNA begin to be expressed, and and with the increase of expression and pathological grade, the occurrence of cervical cancer is further accelerated. At the same time, there is a correlation between the HR-HPV and HR-HPV E6 or E7 mRNA, revealing the consistency between HPV load and the severity of lesions.

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更新日期/Last Update: 1900-01-01