[1]陈 越,彭 莉.黄芪对细胞毒素相关蛋白A诱导的大鼠系膜细胞外基质分泌的影响[J].医学信息官方网站,2022,35(15):104-106.[doi:10.3969/j.issn.1006-1959.2022.15.023]
　CHEN Yue,PENG Li.Effect of Astragalus Membranaceus on CagA-induced Extracellular Matrix Secretion of Rat Mesangial Cells[J].Medical Information,2022,35(15):104-106.[doi:10.3969/j.issn.1006-1959.2022.15.023]
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黄芪对细胞毒素相关蛋白A诱导的大鼠系膜细胞外基质分泌的影响()
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《医学信息》官方网站[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年15期
页码:
104-106
栏目:
论著
出版日期:
2022-08-01

文章信息/Info

Title:
Effect of Astragalus Membranaceus on CagA-induced Extracellular Matrix Secretion of Rat Mesangial Cells
文章编号:
1006-1959（2022）15-0104-04
作者:
陈 越; 彭 莉
（1.自贡市第一人民医院肾内科，四川 自贡 643000；2.成都市第七人民医院肾内科，四川 成都 610000）
Author(s):
CHEN Yue; PENG Li
(1.Department of Nephrology,Zigong First People’s Hospital,Zigong 643000,Sichuan,China;2.Department of Nephrology,Chengdu Seventh People’s Hospital,Chengdu 610000,Sichuan,China)
关键词:
IgA肾病; 黄芪; 系膜细胞; 细胞毒素相关蛋白A
Keywords:
gA nephropathy; Astragalus Membranaceus; Mesangial cells; Cytotoxin-associated protein A
分类号:
R692
DOI:
10.3969/j.issn.1006-1959.2022.15.023
文献标志码:
A
摘要:
目的 研究黄芪对细胞毒素相关蛋白A（cagA）诱导大鼠的系膜细胞外基质分泌的影响。方法 将大鼠系膜细胞进行体外培养，并随机分为四组，分别为空白对照组、IL-1β阳性对照组（10 ng/ml IL-1β）和CagA实验组（4 μg/ml CagA）及CagA+黄芪干预组（1 mg/ml的黄芪注射液+4 μg/ml CagA），各组予以对应处理后刺激培养72 h，检测细胞培养上清液Ⅰ型胶原（CollagenⅠ）、Ⅲ型胶原（CollagenⅢ型）蛋白水平及其mRNA表达。结果 IL-1β阳性对照组、CagA实验组CollagenⅠ、CollagenⅢ蛋白浓度及mRNA表达水平高于空白对照组，CagA+黄芪干预组CollagenⅠ、CollagenⅢ蛋白浓度及mRNA表达水平低于CagA实验组，差异有统计学意义（P＜0.05）。结论 黄芪或通过减少Ⅰ、Ⅲ型胶原蛋白的分泌，抑制CagA诱导的大鼠系膜细胞外基质的异常分泌，可为IgA肾病的治疗提供思路。
Abstract:
Objective To investigate the effects of Astragalus Membranaceus on CagA-induced extracellular matrix secretion of rat mesangial cells.Methods Rat mesangial cells were cultured in vitro and randomly divided into four groups: blank control group, IL-1β positive control group (10 ng/ml IL-1β), CagA experimental group (4 μg/ml CagA) and CagA+Astragalus Membranaceus intervention group (1 mg/ml Astragalus Membranaceus injection+4 μg/ml CagA). Each group was stimulated for 72 h after corresponding treatment. The protein level and mRNA expression of type Ⅰ collagen (CollagenⅠ) and type Ⅲ collagen (CollagenⅢ) in cell culture supernatant were detected.Results The protein concentration and mRNA expression of CollagenⅠ and CollagenⅢin IL-1β positive control group and CagA experimental group were higher than those in blank control group, and the protein concentration and mRNA expression of CollagenⅠ and CollagenⅢ in CagA+Astragalus Membranaceus intervention group were lower than those in CagA experimental group, the difference was statistically significant (P<0.05).Conclusion Astragalus Membranaceus can inhibit CagA-induced abnormal secretion of extracellular matrix in rat mesangial cells by reducing the secretion of type Ⅰ and type Ⅲ collagen, which can provide ideas for the treatment of IgA nephropathy.

参考文献/References:

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更新日期/Last Update: 1900-01-01