[1]陈伟毅,胡 柯,刘 雨,等.气体信号分子硫化氢介导胃癌多药耐药性研究[J].医学信息,2023,36(19):80-83.[doi:10.3969/j.issn.1006-1959.2023.19.016]
　CHEN Wei-yi,HU Ke,LIU Yu,et al.Research on Gaseous Signaling Molecule Hydrogen Sulfide Mediating Multidrug Resistance in Gastric Cancer[J].Journal of Medical Information,2023,36(19):80-83.[doi:10.3969/j.issn.1006-1959.2023.19.016]
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气体信号分子硫化氢介导胃癌多药耐药性研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年19期
页码:
80-83
栏目:
论著
出版日期:
2023-10-01

文章信息/Info

Title:
Research on Gaseous Signaling Molecule Hydrogen Sulfide Mediating Multidrug Resistance in Gastric Cancer
文章编号:
1006-1959（2023）19-0080-04
作者:
陈伟毅; 胡 柯; 刘 雨; 等
（1.湖南医药学院医学院，湖南 怀化 418000；2.湖南医药学院第一附属医院普外科，湖南 怀化 418000）
Author(s):
CHEN Wei-yi; HU Ke; LIU Yu; et al.
(1.School of Medicine,Hunan University of Medicine,Huaihua 418000,Hunan,China;2.Department of General Surgery,the First Affiliated Hospital of Hunan University of Medicine,Huaihua 418000,Hunan,China)
关键词:
H2S; 胃癌; 多药耐药性
Keywords:
H2S; Gastric cancer; Multidrug resistance
分类号:
R735.2
DOI:
10.3969/j.issn.1006-1959.2023.19.016
文献标志码:
A
摘要:
目的 探讨硫化氢（H2S）对胃癌多药耐药性（MDR）的影响。方法 采用逐步递增顺铂浓度法建立胃癌多药耐药细胞株MGC803/MDR，显微镜观察细胞形态，CCK8法检测细胞对顺铂、阿霉素、5-氟尿嘧啶的IC50值，亚甲基蓝分光光度计法检测H2S含量，Western blot检测胱硫醚-β-合成酶（CBS）表达。结果 MGC803/MDR细胞在显微镜下排列松散，胞体较小，细胞形态呈梭形或不规则形。MGC803/MDR细胞对顺铂、阿霉素、5-氟尿嘧啶的IC50值显著高于MGC803细胞（P＜0.01）；MGC803/MDR细胞H2S、CBS表达显著高于MGC803细胞（P＜0.01）；采用氨基氧乙酸（AOAA）作用24 h后，MGC803/MDR细胞H2S生成显著减少（P＜0.01），对顺铂、阿霉素、5-氟尿嘧啶的IC50值显著降低（P＜0.01）。采用NaSH作用24 h后，MGC803细胞对顺铂、阿霉素、5-氟尿嘧啶的IC50值显著升高（P＜0.01）。结论 气体信号分子H2S参与了胃癌多药耐药性的形成。
Abstract:
Objective To investigate the effect of hydrogen sulfide (H2S) on multidrug resistance of gastric cancer.Methods Multidrug resistant gastric cancer cell line MGC803/MDR were established by stepwise dose escalation with cisplatin. Cell morphology was observed under an optical microscope. Cell sensitivity to cisplatin, adriamycin and 5-fluorouracil was analyzed by computing IC50 value which were determined by CCK8 assay. The methylene blue assay was used to detecte H2S. The protein level of cystathionine β synthase (CBS) was detected by Western blot analysis.Results MGC803/MDR cells were loosely arranged with small cell bodies and spindle or irregular cell morphology under microscope observation. The IC50 values of cisplatin, adriamycin and 5-fluorouracil in MGC803/MDR cells were significantly higher than in MGC803 cells (P<0.01). The level of H2S and the protein level of CBS in MGC803/MDR cells were significantly higher than in MGC803 cells (P<0.01). After 24 h treatment with aminoxyacetic acid (AOAA), the production of H2S in MGC803/MDR cells was significantly reduced, and the IC50 values of cisplatin, adriamycin and 5-fluorouracil in MGC803/MDR cells were significantly decreased (P<0.01). After 24 h treatment with NaSH, the IC50 values of cisplatin, adriamycin and 5-fluorouracil in MGC803 cells were significantly increased (P<0.01).Conclusion H2S mediates multidrug resistance of gastric cancer.

参考文献/References:

[1]Peng X,Zha L,Chen A,et al.HOXA5 is a tumor suppressor gene that is decreased in gastric cancer[J].Oncol Rep,2018,40(3):1317-1329.[2]Yao LW,Wu LL,Zhang LH,et al.MFAP2 is overexpressed in gastric cancer and promotes motility via the MFAP2/integrin α5β1/FAK/ERK pathway[J].Oncogenesis,2020,13,9(2):17.[3]Kong FB,Deng QM,Deng HQ,et al.Siva-1 regulates multidrug resistance of gastric cancer by targeting MDR1 and MRP1 via the NF-κB pathway[J].Mol Med Rep,2020,22(2):1558-1566.[4]Kweon Y,Park JY,Kim YJ,et al.Imaging Hydrogen Sulfide in Hypoxic Tissue with [99mTc]Tc-Gluconate[J].Molecules,2020,28,26(1):96.[5]Ascen?觭?觔o K,Lheimeur B,Szabo C.Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells[J].Redox Biol,2022,56:102466.[6]徐婷婷,白伟良,谭海燕,等.雷帕霉素对体外培养的鼻息肉细胞增殖的影响[J].中国医科大学学报,2019,48(11):978-982.[7]许岩,马娜,邓树泳,等.胱硫醚-β-合酶/硫化氢系统在鱼藤酮诱导PC12细胞损伤中的变化[J].中国药理学通报,2014(10):1372-1376.[8]王慧,顾康生.叉头框家族D3和腺苷酸活化蛋白激酶5与胃癌顺铂耐药机制的研究[J].中国临床药理学杂志,2022,38(7):657-660,666.[9]郑培明,李俊蒙,张鹏,等.巨噬细胞外泌体源miR-223促进胃癌细胞对奥沙利铂的耐药研究[J].中国癌症杂志,2021,31(5):368-376.[10]Felipe AV,Oliveira J,Moraes AA,et al.Reversal of Multidrug Resistance in an Epirubicin-Resistant Gastric Cancer Cell Subline[J].Asian Pac J Cancer Prev,2018,19(5):1237-1242.[11]Teng Y,Xuan S,Jiang M,et al.Expression of H2S in Gestational Diabetes Mellitus and Correlation Analysis with Inflammatory Markers IL-6 and TNF-α[J].J Diabetes Res,2020,2020:3085840.[12]Yu Y,Zhou X,Zhu Z,et al.Sodium Hydrosulfide Mitigates Cadmium Toxicity by Promoting Cadmium Retention and Inhibiting Its Translocation from Roots to Shoots in Brassica napus[J].J Agric Food Chem,2019,67(1):433-440.[13]De Cicco P,Ercolano G,Rubino V,et al.Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects[J].Br J Pharmacol,2020,177(4):884-897.[14]Yang N,Liu Y,Li T,et al.Role of Hydrogen Sulfide in Chronic Diseases[J].DNA Cell Biol,2020,39(2):187-196.[15]Untereiner AA,Pavlidou A,Druzhyna N,et al.Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells[J].Biochemical Pharmacology,2018,149:174.[16]Bhattacharyya S,Saha S,Giri K,et al.Cystathionine beta-synthase (CBS) contributes to advanced ovarian cancer progression and drug resistance[J].PLoS One,2013,8(11):e79167.[17]Wang L,Han H,Liu Y,et al.Cystathionine-β-synthase Induces Multidrug Resistance and Metastasis in Hepatocellular Carcinoma[J].Curr Mol Med,2018,18(7):496-506.[18]Sekiguchi F,Sekimoto T,Ogura A,et al.Endogenous Hydrogen Sulfide Enhances Cell Proliferation of Human Gastric Cancer AGS Cells[J].Biol Pharm Bull,2016,39(5):887-890.[19]Choi KS,Song H,Kim EH,et al.Inhibition of Hydrogen Sulfide-induced Angiogenesis and Inflammation in Vascular Endothelial Cells: Potential Mechanisms of Gastric Cancer Prevention by Korean Red Ginseng[J].J Ginseng Res,2012,36(2):135-145.[20]Ye F,Li X,Sun K,et al.Inhibition of endogenous hydrogen sulfide biosynthesis enhances the anti-cancer effect of 3,3’-diindolylmethane in human gastric cancer cells[J].Life Sci,2020,261:118348.

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更新日期/Last Update: 1900-01-01