[1]马靖琳,王 婷,肖红斌,等.基于公共数据库分析FOXM1在软组织肉瘤中的表达及临床预后意义[J].医学信息,2024,37(01):1-9，15.[doi:10.3969/j.issn.1006-1959.2024.01.001]
　MA Jing-lin,WANG Ting,XIAO Hong-bin,et al.Expression and Prognostic Significance of FOXM1 Gene in Soft Tissue Sarcomas Based on the Public Database[J].Journal of Medical Information,2024,37(01):1-9，15.[doi:10.3969/j.issn.1006-1959.2024.01.001]
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基于公共数据库分析FOXM1在软组织肉瘤中的表达及临床预后意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年01期
页码:
1-9，15
栏目:
生物信息学
出版日期:
2024-01-01

文章信息/Info

Title:
Expression and Prognostic Significance of FOXM1 Gene in Soft Tissue Sarcomas Based on the Public Database
文章编号:
1006-1959（2024）01-0001-10
作者:
马靖琳; 王 婷; 肖红斌; 等
（1.深圳市中医肛肠医院<福田>外三科，广东 深圳 518031；2.深圳市福田区风湿病专科医院风湿免疫科，广东 深圳 518000）
Author(s):
MA Jing-lin; WANG Ting; XIAO Hong-bin; et al.
(1.Lanzhou University Second Hospital/Orthopaedics Key Laboratory of Gansu Province,Lanzhou 730030,Gansu,China;2.College of Petrochemical Engineering,Lanzhou University of Technology,Lanzhou 730050,Gansu,China; 3.The Second Clinical Medical College,Lanzhou University,Lanzhou 730030,Gansu,China)
关键词:
克罗恩病; m6A; LncRNA; GSEC
Keywords:
FOXM1; Soft tissue sarcomas; p53 signaling pathway; Biological functions; Cell division; Prognosis
分类号:
R574.62
DOI:
10.3969/j.issn.1006-1959.2024.01.001
文献标志码:
A
摘要:
目的 探索克罗恩病（CD）m6A相关的长链非编码RNA（LncRNA）及其与患者临床性状的相关性。方法 通过GEO数据库获取CD芯片数据，获取m6A相关的甲基化酶的表达量，通过差异分析筛选LncRNA。分析与m6A甲基化酶共表达的LncRNA，探索LncRNA与CD患者病情活动的相关性并绘制ROC曲线，对LncRNA共表达的mRNA进行GO富集分析及KEGG信号通路分析。结果 共筛选出CD中m6A相关的LncRNA 7个，3个LncRNA与8个m6A甲基化酶存在相关性。其中，LncRNAGSEC与病情活动指标CDEIS呈正相关。m6A甲基化位点预测提示GSEC存在多个高甲基化位点；ROC曲线下面积为0.805。GO富集分析显示其富集于蛋白丝氨酸/苏氨酸/酪氨酸激酶活性、Toll 样受体结合等功能。KEGG信号通路分析显示其富集于NF-κB信号通路、炎症性肠病等通路。结论 GSEC可能是CD潜在的诊断及治疗靶点。
Abstract:
Objective To analyze the expression and clinical prognostic value of forkhead box M1 (FOXM1) in soft tissue sarcomas (STSs).Methods The expression level of FOXM1 in common malignant tumors, especially STSs, was analyzed by Oncomine database, and then verified in TCGA database. The expression level of FOXM1 in STSs cell lines was analyzed in CCLE database, and the difference of FOXM1 expression in STSs with gender, race and age groups was analyzed in UALCAN database. The relationship between FOXM1 and the prognosis of STSs patients and its correlation with the expression of key molecules of PI3K/AKT signaling pathway in STSs were analyzed by GEPIA database. The interaction network of FOXM1 interacting proteins was analyzed in the STRING database. Finally, GO functional annotation and KEGG pathway enrichment analysis were performed for FOXM1 and its interacting genes using the online DAVID tools.Results A total of 9 studies in the Oncomine database involved the expression of FOXM1 in STSs tissues and corresponding adjacent tissues, and FOXM1 was highly expressed in STSs (P<0.05). The verification results of the TCGA database were consistent with the mining results of the Oncomine database. FOXM1 was generally highly expressed in STSs cells (P<0.05), and the expression level of FOXM1 was related to gender, but not to race and age. GEPIA database analysis showed that the overall survival(OS) and disease-free survival (DFS) of patients with low expression of FOXM1 were higher than those of patients with high expression (P<0.05). The expression level of FOXM1 in STSs tissues was positively correlated with the expression levels of phosphatidylinositol 3-kinase catalytic subunit α (PIK3CA), AKT1 and mammalian target of rapamycin (mTOR) (r=0.26, 0.43, R=0.42). Protein interaction network analysis showed that CCNB1, PLK1, CDK1, CCNA2, MYBL2, CCNB2, CENPF, CDK2, CDC25A, CDC20 and other proteins had obvious interaction with FOXM1. GO enrichment analysis showed that these genes involved in biological functions including cell division and cell cycle regulation. KEGG enrichment analysis showed that these genes were mainly involved in signaling pathways including p53 signaling pathway, FOXO signaling pathway and PI3K-Akt signaling pathway.Conclusion FOXM1 is highly expressed in soft tissue sarcoma and is related to the prognosis of patients with soft tissue sarcoma. The mechanism may be related to the regulation of PI3K/AKT signaling pathway.

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更新日期/Last Update: 1900-01-01