[1]黄晓东,黄 衔,梁靖华,等.基于m6A相关长链非编码RNA鉴定克罗恩病的潜在诊断生物标志物[J].医学信息,2024,37(01):10-15.[doi:10.3969/j.issn.1006-1959.2024.01.002]
 HUANG Xiao-dong,HUANG Xian,LIANG Jing-hua,et al.Identification of Potential Diagnostic Biomarkers for Crohn’s Disease Based on m6A-related Long Non-coding RNAs[J].Journal of Medical Information,2024,37(01):10-15.[doi:10.3969/j.issn.1006-1959.2024.01.002]
点击复制

基于m6A相关长链非编码RNA鉴定克罗恩病的潜在诊断生物标志物()
分享到:

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年01期
页码:
10-15
栏目:
生物信息学
出版日期:
2024-01-01

文章信息/Info

Title:
Identification of Potential Diagnostic Biomarkers for Crohn’s Disease Based on m6A-related Long Non-coding RNAs
文章编号:
1006-1959(2024)01-0010-06
作者:
黄晓东黄 衔梁靖华
(1.深圳市中医肛肠医院<福田>外三科,广东 深圳 518031;2.深圳市福田区风湿病专科医院风湿免疫科,广东 深圳 518000)
Author(s):
HUANG Xiao-dongHUANG XianLIANG Jing-huaet al.
(1.The Third Department of Surgery,Shenzhen TCM Anorectal Hospital ,Shenzhen 518031,Guangdong,China;2.Department of Rheumatology,Shenzhen Futian District Hospital for Rheumatic Diseases,Shenzhen 518000,Guangdong,China)
关键词:
克罗恩病m6ALncRNAGSEC
Keywords:
Crohn’s diseasem6ALncRNAGSEC
分类号:
R574.62
DOI:
10.3969/j.issn.1006-1959.2024.01.002
文献标志码:
A
摘要:
目的 探索克罗恩病(CD)m6A相关的长链非编码RNA(LncRNA)及其与患者临床性状的相关性。方法 通过GEO数据库获取CD芯片数据,获取m6A相关的甲基化酶的表达量,通过差异分析筛选LncRNA。分析与m6A甲基化酶共表达的LncRNA,探索LncRNA与CD患者病情活动的相关性并绘制ROC曲线,对LncRNA共表达的mRNA进行GO富集分析及KEGG信号通路分析。结果 共筛选出CD中m6A相关的LncRNA 7个,3个LncRNA与8个m6A甲基化酶存在相关性。其中,LncRNAGSEC与病情活动指标CDEIS呈正相关。m6A甲基化位点预测提示GSEC存在多个高甲基化位点;ROC曲线下面积为0.805。GO富集分析显示其富集于蛋白丝氨酸/苏氨酸/酪氨酸激酶活性、Toll 样受体结合等功能。KEGG信号通路分析显示其富集于NF-κB信号通路、炎症性肠病等通路。结论 GSEC可能是CD潜在的诊断及治疗靶点。
Abstract:
Objective To explore the long non-coding RNA (LncRNA) associated with m6A in Crohn’s disease (CD) and its correlation with clinical characteristics of patients.Methods The CD chip data was obtained through the GEO database to obtain the expression of m6A-related methylase, and the LncRNA was screened by differential analysis. The LncRNA co-expressed with m6A methylase was analyzed to explore the correlation between LncRNA and the disease activity of CD patients and draw the ROC curve. GO enrichment analysis and KEGG signaling pathway analysis were performed on the mRNA co-expressed with LncRNA.Results A total of 7 LncRNAs related to m6A in CD were screened, and 3 LncRNAs were correlated with 8 m6A methylases. Among them, LncRNAGSEC was positively correlated with disease activity index CDEIS. The prediction of m6A methylation site suggested that there were multiple hypermethylation sites in GSEC. The area under the ROC curve was 0.805. GO enrichment analysis showed that it was enriched in protein serine/threonine/tyrosine kinase activity, Toll-like receptor binding and other functions. KEGG signaling pathway analysis showed that it was enriched in NF-κB signaling pathway, inflammatory bowel disease and other pathways.Conclusion GSEC may be a potential diagnostic and therapeutic target for CD.

参考文献/References:

[1]Roda G,Chien NG,Kotze PG,et al.Crohn’s disease[J].Nature reviews Disease Primers,2020,6(1):22.[2]郑家驹,史肖华,竺霞霜,等.我国克罗恩病不同年代发病率及患病率的比较[J].中华内科杂志,2011,50(7):597-600.[3]Kuai XY,Yu SY,Cui XF,et al.LncRNA LUCAT1 as a Plasma Biomarker for Assessing Disease Activity in Adult Patients with Crohn’s Disease[J].Gastroenterol Res Pract,2021,2021:5557357.[4]Haberman Y,BenShoshan M,Di Segni A,et al.Long ncRNA Landscape in the Ileum of Treatment-Naive Early-Onset Crohn Disease[J].Inflamm Bowel Dis,2018,24(2):346-360.[5]Wei W,Ji X,Guo X,et al.Regulatory Role of N6 -methyladenosine (m6 A) Methylation in RNA Processing and Human Diseases[J].J Cell Biochem,2017,118(9):2534-2543.[6]Nishizawa Y,Konno M,Asai A,et al.Oncogene c-Myc promotes epitranscriptome m6A reader YTHDF1 expression in colorectal cancer[J].Oncotarget,2017,9(7):7476-7486. [7]Zhu S,Wang JZ,Chen D,et al.An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis[J].Nat Commun,2020,11(1):1685.[8]Oerum S,Meynier V,Catala M,et al.A comprehensive review of m6A/m6Am RNA methyltransferase structures[J].Nucleic Acids Res,2021,49(13):7239-7255.[9]Zhang T,Ding C,Chen H,et al.m6A mRNA modification maintains colonic epithelial cell homeostasis via NF-κB-mediated antiapoptotic pathway[J].Sci Adv,2022,8(12):eabl5723.[10]Sebastian-delaCruz M,Olazagoitia-Garmendia A,Gonzalez-Moro I,et al.Implication of m6A mRNA Methylation in Susceptibility to Inflammatory Bowel Disease[J].Epigenomes,2020,4(3):16.[11]Elamir A,Shaker O,Kamal M,et al.Expression profile of serum LncRNA THRIL and MiR-125b in inflammatory bowel disease[J].PLoS One,2022,17(10):e0275267. [12]Matsumura K,Kawasaki Y,Miyamoto M,et al.The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration[J].Oncogene,2017,36(9):1191-1199.[13]Annunziato F,Cosmi L,Santarlasci V,et al.Phenotypic and functional features of human Th17 cells[J].J Exp Med,2007,204(8):1849-1861.[14]Acosta-Rodriguez EV,Rivino L,Geginat J,et al.Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells[J].Nat Immunol,2007,8(6):639-646.[15]Korn T,Betttlli E,Oukka M,et al.IL-17 and Th17cells[J].Annu Rev Immunol,2009,27:485-517.[16]裴中美,赵振亚,肖静波,等.白细胞介素-17在活动期炎症性肠病患者血浆和肠黏膜中的表达及其意义[J].胃肠病学,2016,21(9):549-553.[17]de Souza HS,Fiocchi C.Immunopathogenesis ofIBD: current state of the art[J].Nat Rev Gastroenterol Hepatol,2016,13(1):13-27.[18]Uhlig HH,Powrie F.Translating immunology intotherapeutic concepts for inflammatory bowel disease[J].Annu Rev Immunol,2018,36:755-781.[19]Zhang C,Liu LW,Sun WJ,et al.Expressions of E-cadherin, p120ctn, β-catenin and NF-κB in ulcerative colitis[J].J Huazhong Univ Sci Technolog Med Sci,2015,35(3):368-373.[20]Han YM,Koh J,Kim JW,et al.NF-kappa B activation correlates with disease phenotype in Crohn’s disease[J].PLoS One,2017,12(7):e0182071.[21]李旺林,刘梦鳌,曹杰,等.TLR4在LPS 诱导的结肠炎症恢复中的作用[J].中国病理生理杂志,2017,33(2):336-343.[22]Lu TX,Zheng Z,Zhang L,et al.A new model of spontaneous colitisin mice induced by deletion of an RNA m(6)A methyltransferasecomponent METTL14 in T cells[J].Cell Mol Gastroenterol Hepatol,2020,10(4):747-761.[23]Wei H,Li Z,Liu F,et al.The Role of FTO in Tumors and Its Research Progress[J].Curr Med Chem,2022,29(5):924-933.[24]Cui J,Wang L,Ren X,et al.LRPPRC:A Multifunctional Protein Involved in Energy Metabolism and Human Disease[J].Front Physiol,2019,10:595.[25]Dragasevic S,Stankovic B,Kotur N,et al.Metabolic syndrome ininflammatory bowel disease: association with genetic markers of obesityand inflammation[J].Metab Syndr Relat Disord,2020,18(1):31-38.[26]Chang JY,Cheon JH.Thiopurine Therapy in Patients With Inflammatory Bowel Disease:A Focus on Metabolism and Pharmacogenetics[J].Dig Dis Sci,2019,64(9):2395-2403.[27]Keller DS,Windsor A,Cohen R,et al.Colorectal cancer in inflammatorybowel disease: review of the evidence[J].Tech Coloproctol,2019,23(1):3-13.[28]Nishio T,Kurabe N,Goto-Inoue N,et al.Immunohistochemical expressionanalysis of leucine-rich PPR-motif-containing protein (LRPPRC), acandidate colorectal cancer biomarker identified by shotgun proteomicsusing iTRAQ[J].Clin Chim Acta,2017,471:276-282.

相似文献/References:

[1]曾 翔,文 星,常一航,等.营养风险筛查和BMI在克罗恩病患者营养评估中的应用[J].医学信息,2019,32(08):133.[doi:10.3969/j.issn.1006-1959.2019.08.039]
 ZENG Xiang,WEN Xing,CHANG Yi-hang,et al.Application of Nutritional Risk Screening and BMI in Nutritional Assessment of Patients with Crohn's Disease[J].Journal of Medical Information,2019,32(01):133.[doi:10.3969/j.issn.1006-1959.2019.08.039]
[2]王莹洁,戴迟兵.益生菌在炎症性肠病中的应用研究[J].医学信息,2020,33(22):42.[doi:10.3969/j.issn.1006-1959.2020.22.013]
 WANG Ying-jie,DAI Chi-bing.Study on the Application of Probiotics in Inflammatory Bowel Disease[J].Journal of Medical Information,2020,33(01):42.[doi:10.3969/j.issn.1006-1959.2020.22.013]
[3]郑鑫辉,崔慧丽,杨铁毅,等.粪乳铁蛋白、维生素D水平与克罗恩病患者骨质疏松的相关性[J].医学信息,2021,34(05):114.[doi:10.3969/j.issn.1006-1959.2021.05.032]
 ZHENG Xin-hui,CUI Hui-li,YANG Tie-yi,et al.Correlation Between Fecal Lactoferrin,Vitamin D Levels and Osteoporosis in Crohn’s Disease[J].Journal of Medical Information,2021,34(01):114.[doi:10.3969/j.issn.1006-1959.2021.05.032]
[4]郑鑫辉,崔慧丽,邵 进,等.克罗恩病患者骨质疏松症的影响因素分析[J].医学信息,2021,34(08):68.[doi:10.3969/j.issn.1006-1959.2021.08.018]
 ZHENG Xin-hui,CUI Hui-li,SHAO Jin,et al.Analysis of Influencing Factors of Osteoporosis in Patients with Crohn’s Disease[J].Journal of Medical Information,2021,34(01):68.[doi:10.3969/j.issn.1006-1959.2021.08.018]
[5]马靖琳,王 婷,肖红斌,等.基于公共数据库分析FOXM1在软组织肉瘤中的表达及临床预后意义[J].医学信息,2024,37(01):1.[doi:10.3969/j.issn.1006-1959.2024.01.001]
 MA Jing-lin,WANG Ting,XIAO Hong-bin,et al.Expression and Prognostic Significance of FOXM1 Gene in Soft Tissue Sarcomas Based on the Public Database[J].Journal of Medical Information,2024,37(01):1.[doi:10.3969/j.issn.1006-1959.2024.01.001]

更新日期/Last Update: 1900-01-01