[1]王啸云,杨健康.黑色素瘤外泌体基因的差异分析和ceRNA网络构建[J].医学信息,2024,37(04):8-13.[doi:10.3969/j.issn.1006-1959.2024.04.002]
　WANG Xiao-yun,YANG Jian-kang.Expression of Exosomal Genes in Melanoma and Construction of ceRNA Network[J].Journal of Medical Information,2024,37(04):8-13.[doi:10.3969/j.issn.1006-1959.2024.04.002]
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黑色素瘤外泌体基因的差异分析和ceRNA网络构建()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年04期
页码:
8-13
栏目:
生物信息学
出版日期:
2024-02-15

文章信息/Info

Title:
Expression of Exosomal Genes in Melanoma and Construction of ceRNA Network
文章编号:
1006-1959（2024）04-0008-06
作者:
王啸云; 杨健康
（大理大学基础医学院，云南 大理 671000）
Author(s):
WANG Xiao-yun; YANG Jian-kang
(School of Basic Medical Sciences,Dali University,Dali 671000,Yunnan,China)
关键词:
黑色素瘤; 外泌体; 差异表达基因
Keywords:
Melanoma; Exosomes; Differentially expressed genes
分类号:
R739.5
DOI:
10.3969/j.issn.1006-1959.2024.04.002
文献标志码:
A
摘要:
目的 分析黑色素瘤（MEL）和健康人外周血外泌体差异表达基因，构建竞争性内源RNA（CeRNA）网络，寻找MEL新的分子机制。方法 从exoRBase 2.0外泌体数据库中下载21例黑色素瘤病人和118名健康人外周血RNA表达数据谱（包括mRNA、lncRNA和circRNA），利用R的limma包和Cytoscape软件找出差异表达基因，预测miRNA后构建CeRNA网络并可视化，并对CeRNA网络中的mRNA进行GO富集分析。结果 筛选出138个差异表达mRNA，135个差异表达lncRNA和33个差异表达circRNA。通过构建CeRNA网络发现，mRNA、lncRNA和circRNA竞争性地结合miRNA，且与miRNA相结合的circRNA表达均下调，mRNA表达大多下调，而lncRNA的表达则大多数呈现上调的趋势。CeRNA网络中的差异表达mRNA GO富集分析显示，这些基因主要与mRNA分解代谢过程的调节等相关。结论 健康人群和MEL患者外周血外泌体具有显著差异性，且mRNA、lncRNA和circRNA可以竞争性地与MEL相关的miRNA结合，实现相互调控。这些CeRNA基因为进一步研究MEL发生发展提供了分子基础，为MEL早期检查和治疗提供新靶点。
Abstract:
Objective To investigate the novel molecular mechanism of melanoma (MEL) exosomes by analyzing differentially expressed genes and constructing a competing endogenous RNA (CeRNA) network.Methods The RNA expression profiles (including mRNA, lncRNA and circRNA) of peripheral blood from 21 melanoma patients and 118 healthy people were downloaded from the exoRBase 2.0 exosome database. The differentially expressed genes were identified by R’s limma package and Cytoscape software. After predicting miRNAs, the CeRNA network was constructed and visualized, and the mRNAs in the CeRNA network were subjected to GO enrichment analysis.Results A total of 138 differentially expressed mRNAs, 135 differentially expressed lncRNAs and 33 differentially expressed circRNAs were screened. Through the construction of CeRNA network, it was found that mRNA, lncRNA and circRNA competitively bind to miRNA, and the expression of circRNA combined with miRNA was down-regulated. The expression of mRNA was mostly down-regulated, while the expression of lncRNA was mostly up-regulated. GO enrichment analysis of differentially expressed mRNAs in the CeRNA network showed that these genes were mainly related to the regulation of mRNA catabolic processes.Conclusion There are significant differences in peripheral blood exosomes between healthy people and MEL patients, and mRNA, lncRNA and circRNA can competitively bind to MEL-related miRNAs to achieve mutual regulation. These CeRNA genes provide a molecular basis for further study of the occurrence and development of MEL, and provide new targets for early detection and treatment of MEL.

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更新日期/Last Update: 1900-01-01