[1]毛孝周,杨 涓,郑 盛.慢性乙型病毒性肝炎患者外周血Th1/Th2相关细胞因子表达的临床意义[J].医学信息,2024,37(07):102-105.[doi:10.3969/j.issn.1006-1959.2024.07.019]
　MAO Xiao-zhou,YANG Juan,ZHENG Sheng.Clinical Significance of Th1/Th2 Related Cytokines Expression in Peripheral Blood of Patients with Chronic Hepatitis B[J].Journal of Medical Information,2024,37(07):102-105.[doi:10.3969/j.issn.1006-1959.2024.07.019]
点击复制

慢性乙型病毒性肝炎患者外周血Th1/Th2相关细胞因子表达的临床意义()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年07期
页码:
102-105
栏目:
论著
出版日期:
2024-04-01

文章信息/Info

Title:
Clinical Significance of Th1/Th2 Related Cytokines Expression in Peripheral Blood of Patients with Chronic Hepatitis B
文章编号:
1006-1959（2024）07-0102-04
作者:
毛孝周; 杨 涓; 郑 盛
（大理大学第二附属医院消化内科，云南 昆明 650011）
Author(s):
MAO Xiao-zhou; YANG Juan; ZHENG Sheng
(Department of Gastroenterology,the Second Affiliated Hospital of Dali University,Kunming 650011,Yunnan,China)
关键词:
乙型病毒性肝炎; IFN-γ; IL-2; IL-4; IL-5; 免疫功能; Th1/Th2相关细胞因子
Keywords:
Viral hepatitis B; IFN-γ; IL-2; IL-4; IL-5; Immune function; Th1/Th2 related cytokines
分类号:
R516.2+2
DOI:
10.3969/j.issn.1006-1959.2024.07.019
文献标志码:
A
摘要:
目的 探讨慢性乙型病毒性肝炎（CHB）患者外周血Th1/Th2相关细胞因子表达水平及临床意义。方法 选取2022年1月-2023年2月大理大学第二附属医院收治的56例CHB患者为研究组，另选取同时期31名体检健康的同龄者为对照组；依据乙肝两对半检测结果将研究组分为HBeAg（+）组与HBeAg（-）组，以病毒载量104拷贝/ml为界值，将HBeAg（+）组进一步分为高病毒载量组（＞104拷贝/ml）与低病毒载量组（＜104拷贝/ml）。检测研究组与对照组外周血Th1/Th2相关细胞因子表达水平，并比较研究组中HBeAg（+）组与HBeAg（-）组Th1/Th2相关细胞因子检测结果，进一步分析HBeAg（+）组中的高病毒载量组与低病毒载量组Th1/Th2相关细胞因子水平差异。结果 研究组外周血IFN-y、IL-2、IL-4、IL-5水平均高于对照组，差异有统计学意义（P＜0.05）；研究组中HBeAg（+）组外周血IFN-γ和IL-2水平低于HBeAg（-）组，而外周血IL-4和IL-5水平高于HBeAg（-）组，差异有统计学意义（P＜0.05）；HBeAg（+）组中低病毒载量组外周血IFN-γ、IL-2、IL-4和IL-5水平均低于高病毒载量组，差异有统计学意义（P＜0.05）。结论 CHB患者存在免疫调节功能紊乱，检测CHB患者外周血Th1/Th2相关细胞因子表达水平对疾病的诊断、病情严重程度的判断及预后具有重要的参考价值。
Abstract:
bjective To investigate the expression level and clinical significance of Th1/Th2 related cytokines in peripheral blood of patients with chronic hepatitis B (CHB).Methods A total of 56 CHB patients admitted to the Second Affiliated Hospital of Dali University from January 2022 to February 2023 were selected as the study group, and 31 healthy peers at the same time were selected as the control group. The study group was divided into HBeAg(+) group and HBeAg(-) group according to the results of two pairs of semi-hepatitis B test. With the viral load of 104 copies/ml as the boundary value, the HBeAg(+) group was further divided into high viral load group (>104 copies/ml) and low viral load group (<104 copies/ml). The expression levels of Th1/Th2-related cytokines in peripheral blood of the study group and the control group were detected, and the results of Th1/Th2-related cytokines in HBeAg(+) group and HBeAg(-) group were compared. The difference of Th1/Th2-related cytokine levels between high viral load group and low viral load group in HBeAg(+) group was further analyzed.Results The levels of IFN-y, IL-2, IL-4 and IL-5 in peripheral blood of the study group were higher than those of the control group, and the differences were statistically significant (P<0.05). In the study group, the levels of IFN-γ and IL-2 in peripheral blood of HBeAg(+) group were lower than those in HBeAg(-) group, while the levels of IL-4 and IL-5 in peripheral blood were higher than those in HBeAg(-) group, the differences were statistically significant (P<0.05). The levels of IFN-γ, IL-2, IL-4 and IL-5 in peripheral blood of low viral load group in HBeAg(+) group were lower than those in high viral load group, and the differences were statistically significant (P<0.05).Conclusion CHB patients have immune regulation dysfunction. The detection of Th1/Th2 related cytokines in peripheral blood of CHB patients has important reference value for the diagnosis, severity and prognosis of the disease.

参考文献/References:

[1]Saraceni C,Birk J.A Review of Hepatitis B Virus and Hepatitis C Virus Immunopathogenesis[J].J Clin Transl Hepatol,2021,9(3):409-418.[2]Santos SA,Lira FS,Silva ET,et al.Effect of moderate exercise under hypoxia on Th1/Th2 cytokine balance[J].The Clinical Respiratory Journal,2019,13(9):583-589.[3]Zhang Q,Huang H,Sun A,et al.Change of Cytokines in Chronic Hepatitis B Patients and HBeAg are Positively Correlated with HBV RNA,Based on Real-world Study[J].J Clin Transl Hepatol,2022,10(3):390-397.[4]王贵强,王福生,庄辉,等.慢性乙型肝炎防治指南(2019年版)[J].临床肝胆病杂志,2019,35(12):41-62.[5]Xie X,Luo J,Broering R,et al.HBeAg induces liver sinusoidal endothelial cell activation to promote intrahepatic CD8 T cell immunity and HBV clearance[J].Cell Mol Immunol,2021,18(11):2572-2574.[6]Bourgine M,Crabe S,Lobaina Y,et al.Nasal route favors the induction of CD4(+) T cell responses in the liver of HBV-carrier mice immunized with a recombinant hepatitis B surface- and core-based therapeutic vaccine[J].Antiviral Research,2018,153:23-32.[7]Spolski R,Li P,Leonard WJ.Biology and regulation of IL-2: from molecular mechanisms to human therapy[J].Nat Rev Immunol,2018,18(10):648-659.[8]Qiu S,He P,Fang X,et al.Significant transcriptome and cytokine changes in hepatitis B vaccine non-responders revealed by genome-wide comparative analysis[J].Hum Vaccin Immunother,2018,14(7):1763-1772.[9]Mao JX,Guo WY,Guo M.Acute rejection after liver transplantation is less common,butpredicts better prognosis in HBV-related hepatocellular carcinoma patients[J].Hepatol Int,2020,14(3):347-361.[10]Ponce LF,Montalvo G,Leon K,et al.Differential Effects of IL2Rα and IL15Rα over the Stability of the Common Beta-Gamma Signaling Subunits of the IL2 and IL15 Receptors[J].J Chem Inf Model,2021,61(4):1913-1920. [11]葛大赫.慢性乙型肝炎及肝硬化、肝癌患者外周血Th1、Th2类细胞因子表达变化及意义[J].中国卫生工程学,2022,21(1):160-162.[12]Clark DN,Begg LR,Filiano AJ.Unique aspects of IFN-γ/STAT1 signaling in neurons[J].Immunol Rev,2022,311(1):187-204.[13]Iwasaki N,Terawaki S,Shimizu K,et al.Th2 cell-derived histamine is involved in nasal Th2 infiltration in mice[J].Inflamm Res,2021,70(5):539-541. [14]唐珂,卢丽霞,钟美佐.利妥昔单抗联合化疗治疗HBsAg阳性的非霍奇金淋巴瘤患者的安全性分析[J].医学临床研究,2015(5):1026-1028.[15]Moreira D,Sampath S,Won H,et al.Myeloid cell-targeted STAT3 inhibition sensitizes head and neck cancers to radiotherapy and Tcell-mediated immunity[J].J Clin Invest,2021,131(2):e137001.[16]袁洪波,何玲玲.孕妇乙肝病毒载量与Th1、Th2及Th17类细胞因子表达的关系探究[J].实用预防医学,2021,28(6):762-765.[17]Chen W,Bian H,Xie X,et al.Negative feedback loop of ERK/CREB/miR-212-3p inhibits HBeAg-induced macrophage activation[J].J Cell Mol Med,2020,24(18):10935-10945.[18]Li MH,Chen QQ,Zhang L,et al.Association of cytokines with hepatitis B virus and its antigen[J].Journal of Medical Virology,2020,92(12):1322-1457.[19]Gao PA,Luo YB,Chen LC,et al.The effect of hepatitis B virus on T lymphocyte and its subsets in chronic hepatitis B patients in different ALT stages: A new concept ALT in HBV infection - ScienceDirect[J].International Immunopharmacology,2021,10(1):108182.

相似文献/References:

[1]邬 剑,李慧玲.ELISA法和胶体金试纸条法在乙肝表面抗原 检测中的应用研究[J].医学信息,2018,31(09):155.[doi:10.3969/j.issn.1006-1959.2018.09.050]
　WU Jian,LI Hui-ling.Application of ELISA and Colloidal Gold Strip in the Detection of Hepatitis B Surface Antigen[J].Journal of Medical Information,2018,31(07):155.[doi:10.3969/j.issn.1006-1959.2018.09.050]
[2]许亚桦.不同证型变应性鼻炎外周血清中维生素D、IL-4及 IFN-γ水平比较[J].医学信息,2018,31(12):80.[doi:10.3969/j.issn.1006-1959.2018.12.024]
　XU Ya-hua.The Levels of Vitamin D IL-4 and IFN- γ in Peripheral Serum of Allergic Rhinitis of Different Syndrome Types[J].Journal of Medical Information,2018,31(07):80.[doi:10.3969/j.issn.1006-1959.2018.12.024]
[3]马 琳,田瑞民.乙型病毒性肝炎相关晚期肝病的抗病毒治疗效果观察[J].医学信息,2019,32(07):120.[doi:10.3969/j.issn.1006-1959.2019.07.036]
　MA Lin,TIAN Rui-min.Observation on the Effect of Antiviral Therapy For Advanced Hepatopathy Associated with Viral Hepatitis B[J].Journal of Medical Information,2019,32(07):120.[doi:10.3969/j.issn.1006-1959.2019.07.036]
[4]张 赓.自身抗体在自身免疫性肝炎和乙型病毒性肝炎中的特征分析[J].医学信息,2020,33(05):173.[doi:10.3969/j.issn.1006-1959.2020.05.059]
　ZHANG Geng.Analysis of the Characteristics of Autoantibodies in Autoimmune Hepatitis and Viral Hepatitis B[J].Journal of Medical Information,2020,33(07):173.[doi:10.3969/j.issn.1006-1959.2020.05.059]
[5]王露露.2019-2021年锦州乙型病毒性肝炎的流行病学特征[J].医学信息,2021,34(24):128.[doi:10.3969/j.issn.1006-1959.2021.24.032]
　WANG Lu-lu.Epidemiological Characteristics of Viral Hepatitis B in Jinzhou from 2019 to 2021[J].Journal of Medical Information,2021,34(07):128.[doi:10.3969/j.issn.1006-1959.2021.24.032]
[6]宋 丹,韩亚凤.2010~2019年天津市河北区乙型病毒性肝炎流行病学分析[J].医学信息,2020,33(16):125.[doi:10.3969/j.issn.1006-1959.2020.16.039]
　SONG Dan,HAN Ya-feng.Epidemiological Analysis of Viral Hepatitis B in Hebei District of Tianjin from 2010 to 2019[J].Journal of Medical Information,2020,33(07):125.[doi:10.3969/j.issn.1006-1959.2020.16.039]
[7]刘 芳,季 良,姜海燕,等.肝爽颗粒联合富马酸替诺福韦二吡呋酯片对乙型病毒性肝炎肝硬化患者APRI、FIB-4的影响[J].医学信息,2021,34(05):104.[doi:10.3969/j.issn.1006-1959.2021.05.029]
　LIU Fang,JI Liang,JIANG Hai-yan,et al.The Effect of Ganshuang Granule Combined with Tenofovir Disoproxil Fumarate Tablets on APRI and FIB-4 in Patients with Hepatitis B Liver Cirrhosis[J].Journal of Medical Information,2021,34(07):104.[doi:10.3969/j.issn.1006-1959.2021.05.029]
[8]成若晨,张 璎,何腾飞,等.原卟啉Ⅸ在HBV相关肝硬化中的临床意义[J].医学信息,2021,34(06):73.[doi:10.3969/j.issn.1006-1959.2021.06.019]
　CHENG Ruo-chen,ZHANG Ying,HE Teng-fei,et al.The Clinical Significance of Protoporphyrin Ⅸ in HBV-related Liver Cirrhosis[J].Journal of Medical Information,2021,34(07):73.[doi:10.3969/j.issn.1006-1959.2021.06.019]
[9]单 昕,艾迎春.白细胞介素-18与疾病的研究[J].医学信息,2021,34(07):53.[doi:10.3969/j.issn.1006-1959.2021.07.015]
　SHAN Xin,AI Ying-chun.Research of Interleukin-18 and Diseases[J].Journal of Medical Information,2021,34(07):53.[doi:10.3969/j.issn.1006-1959.2021.07.015]
[10]谢艳红,冯 莉.左氧氟沙星对老年肺结核患者血清IL-6及IFN-γ水平的影响[J].医学信息,2021,34(16):118.[doi:10.3969/j.issn.1006-1959.2021.16.032]
　XIE Yan-hong,FENG Li.Effect of Levofloxacin on Serum IL-6 and IFN-γ Levels in Elderly Patients with Pulmonary Tuberculosis[J].Journal of Medical Information,2021,34(07):118.[doi:10.3969/j.issn.1006-1959.2021.16.032]

更新日期/Last Update: 1900-01-01