[1]任 芳,陈毅德,冯丽华,等.胃癌细胞系中CD44+胃癌干细胞的表达和意义[J].医学信息,2020,33(20):45-49,53.[doi:10.3969/j.issn.1006-1959.2020.20.013]
 REN Fang,CHEN Yi-de,FENG Li-hua,et al.Expression and Significance of CD44+ Gastric Cancer Stem Cells in Gastric Cancer Cell Lines[J].Medical Information,2020,33(20):45-49,53.[doi:10.3969/j.issn.1006-1959.2020.20.013]
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胃癌细胞系中CD44+胃癌干细胞的表达和意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年20期
页码:
45-49,53
栏目:
论著
出版日期:
2020-10-15

文章信息/Info

Title:
Expression and Significance of CD44+ Gastric Cancer Stem Cells in Gastric Cancer Cell Lines
文章编号:
1006-1959(2020)20-0045-06
作者:
任 芳陈毅德冯丽华
(厦门市海沧医院肿瘤血液内科,福建 厦门 361026)
Author(s):
REN FangCHEN Yi-deFENG Li-huaet al
(Department of Oncology and Hematology,Xiamen Haicang Hospital,Xiamen 361026,Fujian,China)
关键词:
胃癌CD44胃癌干细胞基因表达
Keywords:
Gastric cancerCD44Gastric cancer stem cellsGene expression
分类号:
R735.2
DOI:
10.3969/j.issn.1006-1959.2020.20.013
文献标志码:
A
摘要:
目的 探讨胃癌细胞系中CD44+胃癌干细胞的基因表达及其意义。方法 采用无血清培养基获得漂浮球,在胃腺癌细胞系MKN-45中用FACS检测肿瘤干细胞(CSC)表面标志物CD44的表达,采用FACS分离CD44+亚群,通过体外培养鉴定两种不同细胞群CD44+和CD44-中的致瘤、自我更新、分化特性,采用实时RT-PCR评估CD44+和CD44-中干细胞特异性基因的表达。结果 MKN-45和NCI-N87在无血清培养基中形成胃癌微球体,而KATOⅢ和AGS不能形成胃癌微球体,MKN-45细胞系经FACS分选CD44+和CD44-中细胞,80%MKN-45细胞高表达CD44;CD44+细胞在无血清培养基中显示出更高的微球体形成、分化特性;参与Wnt2、Bmi1、Oct3/4、Notch1、Sox2、Nanog和其他基因的“干细胞”基因的表达水平在CD44+亚群中高于CD44-亚群。结论 人胃癌细胞中的CD44+亚群可能含有胃癌干细胞样细胞,可为胃癌的诊断、治疗提供参考。
Abstract:
Objective To investigate the gene expression and significance of CD44+ gastric cancer stem cells in gastric cancer cell lines.Methods Floating spheres were obtained with serum-free medium. FACS was used to detect the expression of tumor stem cell (CSC) surface marker CD44 in gastric adenocarcinoma cell line MKN-45, CD44+ subgroups were separated by FACS, and two different cell populations were identified by in vitro culture The tumorigenicity, self-renewal, and differentiation characteristics of CD44+ and CD44-. Real-time RT-PCR was used to evaluate the expression of stem cell-specific genes in CD44+ and CD44-.Results MKN-45 and NCI-N87 formed gastric cancer microspheres in serum-free medium, while KATOⅢ and AGS could not form gastric cancer microspheres. The MKN-45 cell line was sorted by FACS for CD44+ and CD44- cells, 80% MKN-45 cells highly express CD44; CD44+ cells show higher microsphere formation and differentiation characteristics in serum-free medium; the expression level of "stem cell" genes involved in Wnt2, Bmi1, Oct3/4, Notch1, Sox2, Nanog and other genes CD44+ subgroup was higher than CD44- subgroup.Conclusion The CD44+ subset of human gastric cancer cells might contain gastric cancer stem cell-like cells, which could provide references for the diagnosis and treatment of gastric cancer.

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更新日期/Last Update: 1900-01-01