[1]屈小亚,张 宇,杨 松,等.肺纤维化相关miRNA筛选及生物信息学分析[J].医学信息,2025,38(03):1-6.[doi:10.3969/j.issn.1006-1959.2025.03.001]
　QU Xiaoya,ZHANG Yu,YANG Song,et al.Screening and Bioinformatics Analysis of miRNAs Related to Pulmonary Fibrosis[J].Journal of Medical Information,2025,38(03):1-6.[doi:10.3969/j.issn.1006-1959.2025.03.001]
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肺纤维化相关miRNA筛选及生物信息学分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
38卷
期数:
2025年03期
页码:
1-6
栏目:
生物信息学
出版日期:
2025-02-01

文章信息/Info

Title:
Screening and Bioinformatics Analysis of miRNAs Related to Pulmonary Fibrosis
文章编号:
1006-1959（2025）03-0001-06
作者:
屈小亚; 张 宇; 杨 松; 阮文雯
厦门医学院呼吸疾病研究所，福建 厦门 361023
Author(s):
QU Xiaoya;  ZHANG Yu;  YANG Song;  RUAN Wenwen
Institute of Respiratory Diseases Xiamen Medical College, Xiamen 361023, Fujian, China
关键词:
肺纤维化; miRNA; 生物信息学; MAPK信号通路
Keywords:
Pulmonary fibrosis;  miRNA;  Bioinformatics;  MAPK signaling pathway
分类号:
R563
DOI:
10.3969/j.issn.1006-1959.2025.03.001
文献标志码:
A
摘要:
目的 探究肺纤维化中miRNA可能的作用机制。方法 从GEO数据库中筛选肺纤维化相关miRNA芯片数据，筛选出两个数据集中共同的差异miRNA，并在相关数据库中预测靶基因与LncRNA。通过GO和KEGG分析上述靶基因的生物学作用，利用STRING数据库构建蛋白互作网络。结果 GSE27430与GSE13316数据集共交集筛选出差异miRNA 12个，其中上调miRNA 5个，下调miRNA 7个。GO富集分析显示，差异基因主要与轴突生成发育、细胞连接、突触组织、早期核内体、神经元突触等有关。KEGG结果显示，差异基因主要涉及MAPK信号通路、Ras信号通路、肿瘤蛋白多糖、轴突导向信号和PLD信号通路等。PPI网络图显示，ACTR2、ACTR3和GNG12等蛋白质与其他蛋白质有超10组联系。结论 肺纤维化相关miRNA筛选及分析可为miRNA在肺纤维化中的发病机制研究提供参考，为肺纤维化治疗拓宽思路。
Abstract:
Objective To explore the possible mechanism of miRNA in pulmonary fibrosis. Methods The miRNA microarray data related to pulmonary fibrosis were screened from the GEO database, and the common differential miRNAs in the two data sets were screened out, and the target genes and LncRNAs were predicted in the relevant databases. The biological functions of the above target genes were analyzed by GO and KEGG, and the protein interaction network was constructed by using STRING database. Results A total of 12 differential miRNAs were screened by the intersection of GSE27430 and GSE13316 data sets, including 5 up-regulated miRNAs and 7 down-regulated miRNAs. GO enrichment analysis showed that the differentially expressed genes were mainly related to axon formation and development, cell connection, synaptic tissue, early nuclear endosome, neuron synapse and so on. KEGG results showed that the differential genes were mainly involved in MAPK signaling pathway, Ras signaling pathway, tumor proteoglycan, axon guidance signal and PLD signaling pathway. The PPI network diagram showed that proteins such as ACTR2, ACTR3 and GNG12 had more than 10 groups of connections with other proteins. Conclusion The screening and analysis of miRNAs related to pulmonary fibrosis can provide reference for the study of the pathogenesis of miRNAs in pulmonary fibrosis, and broaden the thinking for the treatment of pulmonary fibrosis.

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更新日期/Last Update: 1900-01-01