[1]冯晓晨.微生物检验中采用细菌培养与涂片镜检的检出情况对比研究[J].医学信息,2026,39(02):137-140.[doi:10.3969/j.issn.1006-1959.2026.02.026]
　FENG Xiaochen.A Comparative Study on the Detection Situations of Bacterial Cultureand Smear Microscopic Examination in Microbiological Testing[J].Journal of Medical Information,2026,39(02):137-140.[doi:10.3969/j.issn.1006-1959.2026.02.026]
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微生物检验中采用细菌培养与涂片镜检的检出情况对比研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
39卷
期数:
2026年02期
页码:
137-140
栏目:
临床证据信息
出版日期:
2026-01-15

文章信息/Info

Title:
A Comparative Study on the Detection Situations of Bacterial Cultureand Smear Microscopic Examination in Microbiological Testing
文章编号:
1006-1959（2026）02-0137-04
作者:
冯晓晨
连云港市赣榆区疾病预防控制中心检验科，江苏 连云港 221000
Author(s):
FENG Xiaochen
Department of Clinical Laboratory, Ganyu District Center for Disease Control and Prevention, Lianyungang 221000, Jiangsu, China
关键词:
微生物检验; 细菌培养; 涂片镜检; 检出率; 阳性符合率
Keywords:
Microbiological testing;  Bacterial culture;  Smear microscopic examination;  Detection rate;  Positive coincidence rate
分类号:
R446
DOI:
10.3969/j.issn.1006-1959.2026.02.026
文献标志码:
A
摘要:
目的 对比细菌培养与涂片镜检在微生物检验中的检出效果。方法 选取2023年5月-2025年5月连云港市赣榆区疾病预防控制中心接收的160份微生物送检标本作为研究对象，所有标本均依次开展涂片镜检与细菌培养检测。统计标本分布类型及筛查合格率，比较两种方法对革兰阴性菌、革兰阳性菌及真菌的检出率，计算两种方法的总体阳性率及不同类型标本的阳性符合率。结果 160份标本中，合格标本152份，合格率为95.00%，其中痰液标本占比最高（38.75%）。涂片镜检对革兰阴性菌、革兰阳性菌及真菌的检出率分别为72.37%、19.74%、7.89%，细菌培养对应的检出率分别为73.68%、19.08%、7.24%，两种方法检出率比较，差异无统计学意义（P＞0.05）。涂片镜检阳性标本44份，阳性率为28.95%；细菌培养阳性标本47份，阳性率30.92%，两种方法阳性率比较，差异无统计学意义（P＞0.05）。不同类型标本中，痰液标本阳性符合率最高（98.33%），脓液标本阳性符合率最低（86.67%），所有类型标本阳性符合率均在85.00%以上。结论 涂片镜检与细菌培养在微生物检验中的检出效果相当，且涂片镜检具有操作简便、耗时短的优势，可作为细菌培养前的标本筛查手段，两种方法联合应用能为临床提供更高效的支持，值得推广。
Abstract:
Objective To compare the detection effect of bacterial culture and smear microscopy in microbiological examination. Methods From May 2023 to May 2025, 160 microbial submitted specimens received by Ganyu District Center for Disease Control and Prevention of Lianyungang City were selected as the research objects. All specimens were examined by smear microscopy and bacterial culture in turn. The distribution types of specimens and the qualified rate of screening were counted. The detection rates of Gram-negative bacteria, Gram-positive bacteria and fungi by the two methods were compared. The overall positive rates of the two methods and the positive coincidence rates of different types of specimens were calculated. Results Of the 160 specimens, 152 met quality standards, the qualification rate was 95.00%, with sputum samples representing the largest subset (38.75%). The detection rates of Gram-negative bacteria, Gram-positive bacteria and fungi by smear microscopy were 72.37%, 19.74% and 7.89%, respectively; the corresponding detection rates of bacterial culture were 73.68%, 19.08% and 7.24%, respectively; there were no significant difference in the detection rates between the two methods (P＞0.05). There were 44 positive specimens of smear microscopy, with a positive rate of 28.95%; there were 47 positive samples of bacterial culture, with a positive rate of 30.92%; there was no significant difference in the positive rate between the two methods (P＞0.05). Among different types of specimens, the positive coincidence rate of sputum specimens was the highest (98.33%), and the positive coincidence rate of pus specimens was the lowest (86.67%). The positive coincidence rate of all types of specimens was above 85.00%. Conclusion The detection effect of smear microscopy and bacterial culture in microbiological examination is equivalent, and smear microscopy has the advantages of simple operation and short time-consuming. It can be used as a sample screening method before bacterial culture. The combined application of the two methods can provide more efficient support for clinical practice and is worthy of promotion.

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更新日期/Last Update: 1900-01-01