[1]程金来,梁 丹,夏 猛,等.HPLC法测定癌痛消方及其拆方中松脂醇二葡萄糖苷和芍药苷的含量[J].医学信息,2019,32(23):73-77.[doi:10.3969/j.issn.1006-1959.2019.23.020]
　CHENG Jin-Lai,LIANG Dan,XIA Meng,et al.Determination of Rosinol Diglucoside and Paeoniflorin in Cancer Painkiller and Its Decomposed Formula by HPLC[J].Medical Information,2019,32(23):73-77.[doi:10.3969/j.issn.1006-1959.2019.23.020]
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HPLC法测定癌痛消方及其拆方中松脂醇二葡萄糖苷和芍药苷的含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年23期
页码:
73-77
栏目:
论著
出版日期:
2019-12-01

文章信息/Info

Title:
Determination of Rosinol Diglucoside and Paeoniflorin in Cancer Painkiller and Its Decomposed Formula by HPLC
文章编号:
1006-1959（2019）23-0073-05
作者:
程金来; 梁 丹; 夏 猛; 曹子茵; 冷 静
（广西中医药大学基础医学院/广西特色实验动物病症模型重点实验室/广西高发传染病中西医结合转化医学重点实验室培育基地，广西 南宁 530200）
Author(s):
CHENG Jin-Lai; LIANG Dan; XIA Meng; CAO Zi-Yin; LENG Jing
(Basic Medical College of Guangxi University of Traditional Chinese Medicine/Guangxi Key Laboratory of Laboratory Animal Disease Models/Guangxi Key Laboratory of Integrated Traditional Chinese and Western Medicine and Translational Medicine for High Infectious Diseases,Nanning 530200,Guangxi,China)
关键词:
癌痛消方; 含量测定; 松脂醇二葡萄糖苷; 芍药苷; HPLC
Keywords:
Cancer pain elimination; Determination of content; Rosinol diglucoside; Paeoniflorin;  HPLC
分类号:
R286.0
DOI:
10.3969/j.issn.1006-1959.2019.23.020
文献标志码:
A
摘要:
目的 建立癌痛消方及其拆方中松脂醇二葡萄糖苷和芍药苷的测定方法。方法 采用十八烷基硅烷键合硅胶柱（Inertsil ODS-3柱 250×4.6 mm，5 μm）；流动相：乙腈-0.1%磷酸（15∶85）为流动相，进行梯度洗脱；检测波长：230 nm（0~16 min，松脂醇二葡萄糖苷），277 nm（16~30 min，芍药苷）；流速：1.0 ml/min；柱温：30oC。结果 癌痛消-补虚组中松脂醇二葡萄糖苷在1.0~5.0 μg范围内呈现良好的线性关系，r=0.9992，平均回收率为97.23%（RSD=1.31%，n=6）；癌痛消-抑癌组中芍药苷在1.0~5.0 μg范围内呈现良好的线性关系，r=0.9993，平均回收率为97.08%（RSD=1.38%，n=6）；癌痛消-全方中松脂醇二葡萄糖苷在1.0~5.0 μg范围内呈现良好的线性关系，r=0.9992，平均回收率为98.01%（RSD=1.30%，n=6）；癌痛消-全方中芍药苷在1.0~5.0μg范围内呈现良好的线性关系，r=0.9993，平均回收率为96.97%（RSD=1.54%，n=6）；结论 本方法专属性强，准确度高，重现性良好，可用于癌痛消方的质量控制。
Abstract:
Objective To establish a method for the determination of rosinol diglucoside and paeoniflorin in Cancer Decoction and its decomposed formula. Methods An octadecylsilane bonded silica gel column (Inertsil ODS-3 column 250×4.6 mm, 5 μm); mobile phase: acetonitrile-0.1% phosphoric acid (15:85) as mobile phase, gradient elution; detection wavelength: 230 nm (0~16 min, rosinol diglucoside), 277 nm (16~30 min, paeoniflorin); flow rate: 1.0 ml/min; column temperature: 30oC. Results In the cancer pain-compensation group, rosinol diglucoside showed a good linear relationship in the range of 1.0~5.0 μg, r=0.9992, and the average recovery was 97.23% (RSD=1.31%, n=6);The paeoniflorin showed a good linear relationship in the range of 1.0~5.0 μg, r=0.9993, and the average recovery was 97.08% (RSD=1.38%, n=6). Cancer pain elimination-total rosin Diglucoside showed a good linear relationship in the range of 1.0~5.0 μg, r=0.9992, the average recovery was 98.01% (RSD=1.30%, n=6); The cancer has a good linear relationship in the range of 1.0~5.0μg, r=0.9993, and the average recovery rate is 96.97% (RSD=1.54%, n=6). Conclusion This method has strong specificity. High accuracy and good reproducibility, can be used for quality control of cancer pain.

参考文献/References:

[1]农毅清,蒋林,谭安蔷.癌痛消颗粒质量标准研究[J].中国药师,2014(11):1859-1861.[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:105.[3]胥冰,黄峰,韦艾凌,等.癌痛消方对DEN诱癌大鼠不同时间点凋亡相关蛋白的影响[J].华西药学杂志,2017,32(5):489-492.

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更新日期/Last Update: 2019-12-01