[1]吴 玮.HPLC法测定对乙酰氨基酚片的含量[J].医学信息,2018,31(18):127-128,131.[doi:10.3969/j.issn.1006-1959.2018.18.040]
　WU Wei.Determination of Acetaminophen Tablets by HPLC[J].Journal of Medical Information,2018,31(18):127-128,131.[doi:10.3969/j.issn.1006-1959.2018.18.040]
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HPLC法测定对乙酰氨基酚片的含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年18期
页码:
127-128,131
栏目:
药物与临床
出版日期:
2018-09-15

文章信息/Info

Title:
Determination of Acetaminophen Tablets by HPLC
文章编号:
1006-1959（2018）18-0127-03
作者:
吴 玮
安徽省宿州市食品药品检验检测中心，安徽 宿州 234000
Author(s):
WU Wei
Anhui Food and Drug Inspection and Testing Center,Suzhou 234000,Anhui,China
关键词:
HPLC; 对乙酰氨基酚; 含量测定
Keywords:
Key words：HPLC; Acetaminophen; Determination
分类号:
R927.2
DOI:
10.3969/j.issn.1006-1959.2018.18.040
文献标志码:
A
摘要:
目的 建立HPLC法测定对乙酰氨基酚片的含量。方法 色谱柱为Agilent Eclipse XDB-C18柱（4.6 mm×150 mm，5 μm）；流动相为甲醇-水（20∶80），流速为1.0 ml/min，检测波长为244 nm，柱温30 ℃。结果 对乙酰氨基酚浓度在10.16~30.48 μg/ml范围内线性关系良好，r=0.9998（n=5）；平均加样回收率为100.04%，RSD=0.95%（n=6）。结论 HPLC法操作简便、结果准确、重复性好、专属性强，适用于测定对乙酰氨基酚片中对乙酰氨基酚的含量。
Abstract:
Abstract：Objective To establish a HPLC method for the determination of acetaminophen tablets.Methods The column was an Agilent Eclipse XDB-C18 column(4.6 mm×150 mm,5μm);the mobile phase was methanol-water(20:80),the flow rate was 1.0ml/min, the detection wavelength was 244nm,and the column temperature was 30°C.Results The linear relationship of acetaminophen concentration in the range of 10.16~30.48μg/ml was good,r=0.9998(n=5);the average recovery was 100.04%,RSD=0.95%(n=6). Conclusion The HPLC method is simple,accurate,reproducible and specific.It is suitable for the determination of acetaminophen in acetaminophen tablets.

参考文献/References:


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更新日期/Last Update: 2018-09-15