[1]张 瑞,延沁儒.丹酚酸B调节Bax/Bcl-2的表达对阿尔茨海默病小鼠认知功能的影响及机制[J].医学信息,2021,34(17):70-74.[doi:10.3969/j.issn.1006-1959.2021.17.018]
　ZHANG Rui,YAN Qin-ru.Effects of Salvianolic Acid B on the Cognitive Function of Alzheimer’s Disease Mice and its Mechanism Regulating the Expression of Bax/Bcl-2[J].Medical Information,2021,34(17):70-74.[doi:10.3969/j.issn.1006-1959.2021.17.018]
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丹酚酸B调节Bax/Bcl-2的表达对阿尔茨海默病小鼠认知功能的影响及机制()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年17期
页码:
70-74
栏目:
论著
出版日期:
2021-09-01

文章信息/Info

Title:
Effects of Salvianolic Acid B on the Cognitive Function of Alzheimer’s Disease Mice and its Mechanism Regulating the Expression of Bax/Bcl-2
文章编号:
1006-1959（2021）17-0070-05
作者:
张 瑞; 延沁儒
（1.西安国际医学中心医院神经康复科，陕西 西安 710000； 2.空军军医大学第二附属医院康复理疗科，陕西 西安 710000）
Author(s):
ZHANG Rui; YAN Qin-ru
(1.Department of Neurorehabilitation,Xi’an International Medical Center Hospital,Xi’an 710000, Shaanxi,China; 2.Department of Rehabilitation and Physiotherapy,the Second Affiliated Hospital of Air Force Military Medical University, Xi’an 710000,Shaanxi,China)
关键词:
丹酚酸B; 阿尔茨海默病; 认知功能; 氧化应激; 凋亡
Keywords:
Salvianolic acid B; Alzheimer’s disease; Cognitive function; Oxidative stress; Apoptosis
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2021.17.018
文献标志码:
A
摘要:
目的 研究丹酚酸B（Sal B）对D-半乳糖联合氯化铝诱导的阿尔茨海默病（AD）小鼠认知功能保护作用和机制。方法 将6月龄SPF级小鼠随机分为对照组、AD模型组、Sal B低剂量组（30 mg/kg）、Sal B高剂量组（60 mg/kg），每组10只，连续灌胃给药8周。采用跳台实验观察各组小鼠认知和记忆能力；比色法检测AD小鼠血清及海马的氧化指标超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）以及丙二醛（MDA）；Western Blot 法检测半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）、B淋巴细胞瘤-2 基因（Bcl-2）、Bcl-2相关X蛋白（Bax）表达并比较。结果 Sal B各组较AD模型组认知功能及记忆水平改善，SOD和GSH-PX 活力升高、MDA 水平降低，Caspase-3、Bax、Bax/Bcl-2表达减少，Bcl-2表达增加。结论 Sal B对AD小鼠认知功能具有一定的保护作用，其可能的机制是改善氧化应激损伤，减少神经元凋亡。
Abstract:
Objective To study the protective effect and mechanism of salvianolic acid B (Sal B) on the cognitive function of Alzheimer’s disease (AD) mice induced by D-galactose combined with aluminum chloride.Methods 6-month-old SPF mice were randomly divided into control group, AD model group, Sal B low-dose group (30 mg/kg), and Sal B high-dose group (60 mg/kg), each group had 10 mice，they were administered intragastrically for 8 weeks.Using platform jumping experiment to observe the cognitive and memory abilities of mice in each group;Colorimetric detection of AD mouse serum and hippocampus oxidation indicators superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA);Western Blot was used to detect the expression of Caspase-3 (Caspase-3), B lymphocyte tumor-2 (Bcl-2), and Bcl-2 related X protein (Bax) and compare them.Results Compared with the AD model group, the Sal B group improved cognitive function and memory level, SOD and GSH-PX activity increased, and MDA level decreased.The expression of Caspase-3, Bax, Bax/Bcl-2 decreased, and the expression of Bcl-2 increased.Conclusion SalB has a certain protective effect on the cognitive function of AD mice, and its possible mechanism is to improve oxidative stress damage and reduce neuronal apoptosis.

参考文献/References:

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更新日期/Last Update: 1900-01-01