[1]逄亚宁,王 聪,刘 奇,等.结核分枝杆菌BfrA蛋白的生物信息学分析[J].医学信息,2022,35(06):81-85.[doi:10.3969/j.issn.1006-1959.2022.06.020]
　PANG Ya-ning,WANG Cong,LIU Qi,et al.Bioinformatics Analysis of BfrA Protein in Mycobacterium Tuberculosis[J].Medical Information,2022,35(06):81-85.[doi:10.3969/j.issn.1006-1959.2022.06.020]
点击复制

结核分枝杆菌BfrA蛋白的生物信息学分析()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年06期
页码:
81-85
栏目:
论著
出版日期:
2022-03-15

文章信息/Info

Title:
Bioinformatics Analysis of BfrA Protein in Mycobacterium Tuberculosis
文章编号:
1006-1959（2022）06-0081-05
作者:
逄亚宁; 王 聪; 刘 奇; 等
（大理大学基础医学院医学微生物与免疫学教研室，云南 大理 671000）
Author(s):
PANG Ya-ning; WANG Cong; LIU Qi; et al.
(Department of Medical Microbiology and Immunology,Basic Medical College,Dali University,Dali 671000,Yunnan,China)
关键词:
结核分枝杆菌; BfrA; Rv1876; 生物信息学
Keywords:
Mycobacterium tuberculosis; BfrA; Rv1876; Bioinformatics
分类号:
R516
DOI:
10.3969/j.issn.1006-1959.2022.06.020
文献标志码:
A
摘要:
目的 运用生物信息学方法预测和分析结核分枝杆菌BfrA蛋白的结构和功能。方法 从NCBI数据库中检索基因Rv1876及其编码蛋白BfrA的基本信息，通过NCBI Blastp工具分析BfrA蛋白与人类蛋白的同源性，采用ProtParam、ProtScale、SOSUI、TMHMM、SignalP、TargetP、PSORT、Motif Scan、SOPMA、SWISS-MODEL、BepiPred、ABCpred、NetMHCII、NetMHCIIpan、NetCTL、SYFPEITHI和STRING生物信息学在线软件预测及分析其理化性质、亲疏水性、可溶性、跨膜区、信号肽、亚细胞定位、翻译后修饰位点、二级结构、三级结构、B细胞及T细胞抗原表位、蛋白相互作用网络。结果 BfrA蛋白分子式为C802H1276N218O259S7，含有159个氨基酸，理论PI值为4.50，具有稳定性，是一种亲水性可溶性蛋白质；不具有信号肽和跨膜区域，是定位于细胞质的非分泌蛋白；BfrA蛋白二级结构包含α-螺旋（Hh）、β-转角（Tt）、无规则卷曲（Cc）、β-折叠（Ee）；含有4个CK2-磷酸化位点、1个豆蔻酰化位点、3个PKC-磷酸化位点、1个TYR-磷酸化位点；该蛋白含有多个B细胞、Th细胞及CTL细胞抗原优势表位；与hemZ、mmcO、Rv1877、bfrB等蛋白形成相互作用网络。结论 通过生物信息学技术预测到BfrA蛋白具有多个B细胞及T细胞抗原表位，在结核病的预防和诊断方面具有应用前景。
Abstract:
Objective To predict and analyze the structure and function of the BfrA protein from Mycobacterium tuberculosis by bioinformatics methods.Methods The basic information of gene Rv1876 and its encoded protein BfrA were retrieved from NCBI database, and the homology between BfrA protein and human protein was analyzed by NCBI Blastp tool. ProtParam, ProtScale, SOSUI, TMHMM, SignalP, TargetP, PSORT, Motif Scan, SOPMA, SWISS-MODEL, BepiPred, ABCpred, NetMHCII, NetMHCIIpan, NetCTL, SYFPEITHI and STRING bioinformatics online softwares were used to predict and analyze their physicochemical properties, hydrophobicity, solubility, transmembrane region, signal peptide, subcellular localization, post-translational modification site, secondary structure, tertiary structure, B cell and T cell antigen epitopes and protein-protein interaction network.Results The molecular formula of BfrA protein was C802H1276N218O259S7, containing 159 amino acids, the theoretical PI value was 4.50, it was stable and hydrophilic soluble protein. No signal peptide and transmembrane region, which was located in the cytoplasm of nonsecretory protein. The secondary structure of BfrA protein included α-helix (Hh), β-turn (Tt), random coil (Cc), and β-sheet (Ee); there were four CK2-phosphorylation sites, one nutmeg acylation site, three PKC-phosphorylation sites and one TYR-phosphorylation site; the protein contained multiple dominant epitopes of B cells, Th cells and CTL cells; the interaction network was formed with proteins such as hemZ, mmcO, Rv1877 and bfrB.Conclusion BfrA protein has multiple B cell and T cell epitopes predicted by bioinformatics technology, which has application prospect in the prevention and diagnosis of tuberculosis.

参考文献/References:

[1]Lam A,Prabhu R,Gross C M,et al.Role of apoptosis and autophagy in tuberculosis[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2017,313(2):L218-L229.[2]Ehrt S,Schnappinger D,Rhee KY.Metabolic principles of persistence and pathogenicity in Mycobacterium tuberculosis[J].Nat Rev Microbiol,2018,16(8):496-507[3]Chakaya J,Khan M,Ntoumi F,et al.Global Tuberculosis Report 2020- Reflections on the Global TB burden, treatment and prevention efforts[J].Int J Infect Dis,2021,113(Suppl 1):S7-S12.[4]Khare G,Nangpal P,Tyagi AK.Differential Roles of Iron Storage Proteins in Maintaining the Iron Homeostasis in Mycobacterium tuberculosis[J].PLoS One,2017,12(1):e169545.[5]李琳雪.结核分枝杆菌Rv1860、Rv0494及Rv1876蛋白在活动性结核病诊断中的临床应用研究[D].遵义:遵义医科大学,2020.[6]Singh R,Dwivedi SP,Gaharwar US,et al.Recent updates on drug resistance inMycobacterium tuberculosis[J].Journal of Applied Microbiology,2020,128(6):1547-1567.[7]Peters JS,Andrews JR,Hatherill M,et al.Advances in the understanding of Mycobacterium tuberculosis transmission in HIV-endemic settings[J].The Lancet Infectious Diseases,2019,19(3):e65-e76.[8]Mustafa AD,Kalyanasundram J,Sabidi S,et al.Proof of concept in utilizing in-trans surface display system of Lactobacillus plantarum as mucosal tuberculosis vaccine via oral administration in mice[J].BMC Biotechnology,2018,18(1):63.[9]Ranjbar MM,Ebrahimi MM,Shahsavandi S,et al.Novel Applications of Immuno-bioinformatics in Vaccine and Bio-product Developments at Research Institutes[J].Arch Razi Inst,2019,74(3):219-233.[10]Kozakiewicz L,Phuah J,Flynn J,et al.The role of B cells and humoral immunity in Mycobacterium tuberculosis infection[J].Adv Exp Med Biol,2013(783):225-250.[11]Kawahara JY,Irvine EB,Alter G.A Case for Antibodies as Mechanistic Correlates of Immunity in Tuberculosis[J].Frontiers in Immunology,2019(10):996[12]Jurewicz MM,Stern LJ.Class Ⅱ MHC antigen processing in immune tolerance and inflammation[J].Immunogenetics,2019,71(3):171-187.[13]Jasenosky LD,Scriba TJ,Hanekom WA,et al.T cells and adaptive immunity toMycobacterium tuberculosis in humans[J].Immunological Reviews,2015,264(1):74-87.[14]Orlando V,La Manna MP,Goletti D,et al.Human CD4 T-Cells With a Naive Phenotype Produce Multiple Cytokines During Mycobacterium Tuberculosis Infection and Correlate With Active Disease[J].Front Immunol,2018(9):1119.[15]Yuan C,Qu ZL,Tang XL,et al.Mycobacterium tuberculosis Mannose-Capped Lipoarabinomannan Induces IL-10-Producing B Cells and Hinders CD4+ Th1 Immunity[J].iScience,2019(11):13-30.[16]Farsida,Shabariah R,Hatta M,et al.Relationship between expression mRNA gene Treg, Treg, CD4+, and CD8+ protein levels with TST in tuberculosis children: A nested case-control[J].Ann Med Surg (Lond),2020(61):44-47.[17]Bohme J,Martinez N,Li S,et al.Metformin enhances anti-mycobacterial responses by educating CD8+ T-cell immunometabolic circuits[J].Nat Commun,2020,11(1):5225.[18]Yang JD,Mott D,Sutiwisesak R,et al.Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages[J].PLoS Pathog,2018,14(5):e1007060.[19]Pomaznoy M,Kuan R,Lindvall M,et al.Quantitative and Qualitative Perturbations of CD8+ MAITs in Healthy Mycobacterium tuberculosis-Infected Individuals[J].Immunohorizons,2020,4(6):292-307.[20]Rai PK,Chodisetti SB,Maurya SK,et al.A lipidated bi-epitope vaccine comprising of MHC-Ⅰ and MHC-Ⅱ binder peptides elicits protective CD4 T cell and CD8 T cell immunity against Mycobacterium tuberculosis[J].J Transl Med,2018,16(1):279.[21]Zaitouna AJ,Kaur A,Raghavan M.Variations in MHC class Ⅰ antigen presentation and immunopeptidome selection pathways[J].F1000Research,2020(9):1177.[22]Lin PL,Flynn JL.CD8 T cells and Mycobacterium tuberculosis infection[J].Seminars in Immunopathology,2015,37(3):239-249.[23]Chavez-Galan L,Illescas-Eugenio J,Alvarez-Sekely M,et al.Tuberculosis patients display a high proportion of CD8(+) T cells with a high cytotoxic potential[J].Microbiol Immunol,2019,63(8):316-327.[24]Green AM,Difazio R,Flynn JL.IFN-γ from CD4 T cells is essential for host survival and enhances CD8 T cell function during Mycobacterium tuberculosis infection[J].J Immunol,2013,190(1):270-277.[25]Hao X,Bai J,Ding Y,et al.Characterization of antibody response against 16kD and 38kD of M.tuberculosis in the assisted diagnosis of active pulmonary tuberculosis[J].Annals of Translational Medicine,2020,8(15):945.[26]Ma Y,Li R,Shen J,et al.Clinical effect of T-SPOT.TB test for the diagnosis of tuberculosis[J].BMC Infectious Diseases,2019,19(1):993.[27]Sable SB,Posey JE,Scriba TJ.Tuberculosis Vaccine Development: Progress in Clinical Evaluation[J].Clinical Microbiology Reviews,2019,33(1):e00100-19.

相似文献/References:

[1]刘德福.肺结核患者痰中结核分枝杆菌检验的临床价值分析[J].医学信息,2018,31(01):151.[doi:10.3969/j.issn.1006-1959.2018.01.059]
　LIU De-fu.Clinical Value Analysis of Mycobacterium tuberculosis in Sputum of Patients with Pulmonary Tuberculosis[J].Medical Information,2018,31(06):151.[doi:10.3969/j.issn.1006-1959.2018.01.059]
[2]陈 波,马晓波,洪国粦.Xpert MTB/RIF技术对结核分枝杆菌的诊断价值[J].医学信息,2019,32(06):172.[doi:10.3969/j.issn.1006-1959.2019.06.056]
　CHEN Bo,MA Xiao-bo,HONG Guo-lin.Diagnostic Value of Xpert MTB/RIF Technique for Mycobacterium Tuberculosis[J].Medical Information,2019,32(06):172.[doi:10.3969/j.issn.1006-1959.2019.06.056]
[3]胡立珍,周广信,霍丽丽,等.全自动半巢式实时荧光定量PCR技术检测 BALF诊断肺结核的价值[J].医学信息,2019,32(15):165.[doi:10.3969/j.issn.1006-1959.2019.15.056]
　HU Li-zhen,ZHOU Guang-xin,HUO Li-li,et al.Detection of the Value of BALF in the Diagnosis of Pulmonary Tuberculosis by Automatic Semi-nested Real-time PCR[J].Medical Information,2019,32(06):165.[doi:10.3969/j.issn.1006-1959.2019.15.056]
[4]李 鑫.肺结核合并气管支气管结核危险因素及诊断[J].医学信息,2020,33(01):32.[doi:10.3969/j.issn.1006-1959.2020.01.012]
　LI Xin.Risk Factors and Diagnosis of Tuberculosis with Tracheobronchial Tuberculosis[J].Medical Information,2020,33(06):32.[doi:10.3969/j.issn.1006-1959.2020.01.012]
[5]徐 玥,王 璞.吸烟对肺结核发病、临床特征及转归的影响[J].医学信息,2021,34(05):54.[doi:10.3969/j.issn.1006-1959.2021.05.016]
　XU Yue,WANG Pu.The Effect of Smoking on the Incidence,Clinical Characteristics and Outcome of Tuberculosis[J].Medical Information,2021,34(06):54.[doi:10.3969/j.issn.1006-1959.2021.05.016]
[6]黄 丹,王 璞.肉芽肿性肺疾病的研究现状[J].医学信息,2022,35(05):44.[doi:10.3969/j.issn.1006-1959.2022.05.011]
　HUANG Dan,WANG Pu.Research Status of Granulomatous Lung Disease[J].Medical Information,2022,35(06):44.[doi:10.3969/j.issn.1006-1959.2022.05.011]
[7]曾冬梅.血清中结核分枝杆菌IgG、IgM抗体检测在肺结核诊断中的应用价值[J].医学信息,2022,35(22):157.[doi:10.3969/j.issn.1006-1959.2022.22.039]
　ZENG Dong-mei.The Application Value of Serum Mycobacterium Tuberculosis IgG and IgM Antibody Detection in the Diagnosis of Pulmonary Tuberculosis[J].Medical Information,2022,35(06):157.[doi:10.3969/j.issn.1006-1959.2022.22.039]
[8]杨晓悦,李毓龙,罗仲秋,等.结合分枝杆菌感染者辅助性Th2型细胞因子IL-4、IL-6、IL-10的水平变化[J].医学信息,2023,36(01):113.[doi:10.3969/j.issn.1006-1959.2023.01.022]
　YANG Xiao-yue,LI Yu-long,LUO Zhong-qiu,et al.Changes of Helper Th2 Cytokines IL-4, IL-6 and IL-10 Levels in Patients with Mycobacterium Tuberculosis Infection[J].Medical Information,2023,36(06):113.[doi:10.3969/j.issn.1006-1959.2023.01.022]
[9]于 江,孙 杰,刘凤君.结核分枝杆菌抗原在结核病病理学诊断中的研究进展[J].医学信息,2023,36(08):170.[doi:10.3969/j.issn.1006-1959.2023.08.038]
　YU Jiang,SUN Jie,LIU Feng-jun.Research Progress of Mycobacterium Tuberculosis Antigen in Pathological Diagnosis of Tuberculosis[J].Medical Information,2023,36(06):170.[doi:10.3969/j.issn.1006-1959.2023.08.038]
[10]高 灿,赵晓彤,程 江.结核分枝杆菌TB10.4蛋白的生物信息学分析[J].医学信息,2023,36(18):1.[doi:10.3969/j.issn.1006-1959.2023.18.001]
　GAO Can,ZHAO Xiao-tong,CHENG Jiang.Bioinformatics Analysis of Mycobacterium Tuberculosis TB10.4 Protein[J].Medical Information,2023,36(06):1.[doi:10.3969/j.issn.1006-1959.2023.18.001]

更新日期/Last Update: 1900-01-01