[1]周 梅,赵 刚,禤瑞霞,等.鼻咽癌中IKKβ和Ki-67的表达与肿瘤侵袭转移的关系[J].医学信息,2022,35(17):22-26.[doi:10.3969/j.issn.1006-1959.2022.17.005]
 ZHOU Mei,ZHAO Gang,XUN Rui-xia,et al.Expression of IKKβ and Ki-67 in Nasopharyngeal Carcinoma and its Relationship with Tumor Invasion and Metastasis[J].Journal of Medical Information,2022,35(17):22-26.[doi:10.3969/j.issn.1006-1959.2022.17.005]
点击复制

鼻咽癌中IKKβ和Ki-67的表达与肿瘤侵袭转移的关系()
分享到:

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年17期
页码:
22-26
栏目:
论著
出版日期:
2022-09-01

文章信息/Info

Title:
Expression of IKKβ and Ki-67 in Nasopharyngeal Carcinoma and its Relationship with Tumor Invasion and Metastasis
文章编号:
1006-1959(2022)17-0022-05
作者:
周 梅赵 刚 禤瑞霞
(中山市中医院病理科1,超声科2,广东 中山 528400)
Author(s):
ZHOU MeiZHAO GangXUN Rui-xiaet al.
(Departerment of Pathology1,Departerment of Ultrasonography2,Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,Guangdong,China)
关键词:
IKKβKi-67鼻咽癌免疫组化
Keywords:
IKKβKi-67Nasopharyngeal cancerImmunohistochemistry
分类号:
R739.63
DOI:
10.3969/j.issn.1006-1959.2022.17.005
文献标志码:
A
摘要:
目的 观察鼻咽癌和鼻咽炎组织中IKKβ蛋白及mRNA的表达变化,分析其与患者临床病理指标及Ki-67的关系。方法 收集2018年1月-2019年12月中山市中医院病理科鼻咽癌组织标本60份和鼻咽炎组织标本30份,采用免疫组化和实时荧光定量PCR技术检测IKKβ、Ki-67蛋白及IKKβmRNA在鼻咽癌和鼻咽炎组织中的相对表达量,分析IKKβ表达与鼻咽癌患者临床病理指标和Ki-67的关系。结果 IKKβ和Ki-67蛋白在鼻咽癌组织中的表达高于鼻咽炎组织,差异有统计学意义(P<0.05);在鼻咽癌组织中IKKβ与Ki-67蛋白的表达呈正相关(r=0.262,P<0.05);IKKβ蛋白及mRNA的相对表达量与鼻咽癌肿瘤大小、淋巴结转移及TNM分期具有相关性,差异有统计学意义(P<0.05),而与患者的年龄、性别、组织学分型无关(P>0.05)。结论 IKKβ和Ki-67蛋白在鼻咽癌中表达增高,IKKβ蛋白和mRNA的相对表达量与鼻咽癌肿瘤体积、淋巴结转移和TNM分期密切相关,IKKβ可能作为判断鼻咽癌侵袭转移能力的有效指标。
Abstract:
Objective To observe the expression of IKKβ protein and mRNA in nasopharyngeal carcinoma and nasopharyngitis, and to analyze its relationship with clinicopathological parameters and Ki-67.Methods From January 2018 to December 2019, 60 nasopharyngeal carcinoma tissue specimens and 30 nasopharyngitis tissue specimens were collected from the Department of Pathology, Zhongshan Hospital of Traditional Chinese Medicine. The relative expression of IKKβ, Ki-67 protein and IKKβ mRNA in nasopharyngeal carcinoma and nasopharyngitis tissues was detected by immunohistochemistry and real-time fluorescence quantitative PCR. The relationship between IKKβ expression and clinicopathological parameters and Ki-67 in patients with nasopharyngeal carcinoma was analyzed.Results The expression of IKKβ and Ki-67 protein in nasopharyngeal carcinoma was higher than that in nasopharyngitis,the difference was statistically significant (P<0.05).There was a positive correlation between the expression of IKKβ and Ki-67 protein in nasopharyngeal carcinoma (r=0.262, P<0.05).The relative expression of IKKβ protein and mRNA was correlated with tumor size, lymph node metastasis and TNM stage of nasopharyngeal carcinoma, the difference was statistically significant(P<0.05), whileit was not related to the age, sex and histological type of the patients (P>0.05).Conclusion The expression of IKKβ and Ki-67 protein is increased in nasopharyngeal carcinoma. The relative expression of IKKβ protein and mRNA is closely related to tumor volume, lymph node metastasis and TNM stage of nasopharyngeal carcinoma. IKKβ may be used as an effective index to judge the invasion and metastasis of nasopharyngeal carcinoma.

参考文献/References:

[1]Xue N,Xing S,Ma WG,et al.Combination of plasma MIF and VCA-IgA improves the diagnostic specificity for patients with nasopharyngeal carcinoma[J].Technol Cancer Res Treat,2020,19:1533033820935773.[2]Zhou JC,Zhang JJ,Xu M,et al.High SRC-1 and Twist1 expression predicts poor prognosis and promotes migration and invasion by inducing epithelial-mesenchymal transition in human nasopharyngeal carcinoma[J].PLoS One,2019,14(4):e0215299.[3]Lai SZ,Li WF,Chen L,et al.How does intensity-modulated radiotherapy versus conventional two-dimensional radiotherapy influence the treatment results in nasopharyngeal carcinoma patients?[J].Int J Radiat Oncol Biol Phys,2011,80(3):661-668.[4]Perkins ND.Integrating cell-signalling pathways with NF-kappaB and IKK function[J].Nat Rev Mol Cell Biol,2007,8(1):49-62.[5]Rudolph D,Yeh WC,Wakeham A,et al.Severe liver degeneration and lack of NF-kappaB activation in NEMO/IKKgamma-deficient mice[J].Genes Dev,2000,14(7):854-862.[6]Yang CW,Liu XJ,Zhao L,et al.Preoperative prediction of gastrointestinal stromal tumors with high Ki-67 proliferation index based on CT features[J].Ann Transl Med,2021,9(20):1556.[7]Zheng XH,Wang RZ,Li XZ,et al.Detection of methylation status of Epstein-Barr virus DNA C promoter in the diagnosis of nasopharyngeal carcinoma[J].Cancer Sci,2020,111(2):592-600.[8]DiDonato JA,Mercurio F,Karin M.NF-κB and the link between inflammation and cancer[J].Immunol Rev,2012,246(1):379-400.[9]Yin MZ,Lu JW,Guo ZZ,et al.Reduced SULT2B1b expression alleviates ox-LDL-induced inflammation by upregulating miR-148-3P via inhibiting the IKKβ/NF-κB pathway in macrophages[J].Aging,2021,13(3):3428-3442.[10]Hayden MS,Ghosh S.Shared principles in NF-κB signaling[J].Cell,2008,132(3):344-362.[11]Yang SD,Deng QF,Sun LQ,et al.Salmonellaeffector SpvBinhibits NF-κBactivity via KEAP1-mediated downregulation of IKKβ[J].Front Cell Infect Microbiol,2021,11:641412.[12]Taniguchi K,Karin M.NF-κB, inflammation, immunity and cancer: coming of age[J].Nat Rev Immunol,2018,18(5):309-324.[13]Prescott JA,Cook SJ.Targeting IKKβ in cancer: challenges and opportunities for the therapeutic utilisation of IKKβ inhibitors[J].Cells,2018,7(9):115.[14]Barnabei L,Laplantine E,Mbongo W,et al.NF-κB: At the borders of autoimmunity and inflammation[J].Front Immunol,2021,12:716469.[15]Chung GT,Lou WP,Chow C,et al.Constitutive activation of distinct NF-κB signals in EBV-associated nasopharyngeal carcinoma[J].J Pathol,2013,231(3):311-322.[16]Shair KHY,Reddy A,Cooper VS.New insights from elucidating the role of LMP1 in nasopharyngeal carcinoma[J].Cancers,2018,10(4):86.[17]Li S,Lv MY,Qiu S,et al.NF-κB p65 promotes ovarian cancer cell proliferation and migration via regulating mortalin[J].J Cell Mol Med,2019,23(6):4338-4348.[18]Wu WX,Jiang GH,Xu ZF,et al.Three-dimensional pulsed continuous arterial spin labeling and intravoxel incoherent motion imaging of nasopharyngeal carcinoma: correlations with Ki-67 proliferation status[J].Quant Imaging Med Surg,2021,11(4):1394-1405.[19]Lung HL,Kan R,Chau WY,et al.The anti-tumor function of the IKK inhibitor PS1145 and high levels of p65 and KLF4 are associated with the drug resistance in nasopharyngeal carcinoma cells[J].Sci Rep,2019,9(1):12064.[20]Xing YX,Lin YN,Zhang Y,et al.Novel cytoplasmic lncRNA IKBKBAS promotes lung adenocarcinoma metastasis by upregulating IKKβ and consequential activation of NF-κB signaling pathway[J].Cell Death Dis,2021,12:1004.[21]Liang YS,Feng GF,Wu L,et al.Caffeic acid phenethyl ester suppressed growth and metastasis of nasopharyngeal carcinoma cells by inactivating the NF-κB pathway[J].Drug Des Devel Ther,2019,13:1335-1345.

相似文献/References:

[1]史浩明,吴庆琛.ⅠB期肺腺癌术后复发转移因素研究[J].医学信息,2021,34(04):92.[doi:10.3969/j.issn.1006-1959.2021.04.024]
 SHI Hao-ming,WU Qing-chen.Study on the Factors of Postoperative Recurrence and Metastasis of Stage ⅠB Lung Adenocarcinoma[J].Journal of Medical Information,2021,34(17):92.[doi:10.3969/j.issn.1006-1959.2021.04.024]
[2]陈余英,周小花,陈晓文,等.乳腺浸润性导管癌中ER、PR、Her-2、Ki-67表达与病理特征及预后的相关性[J].医学信息,2020,33(01):68.[doi:10.3969/j.issn.1006-1959.2020.01.022]
 CHEN Yu-ying,ZHOU Xiao-hua,CHEN Xiao-wen,et al.Correlation of ER,PR,Her-2,Ki-67 Expression with Pathological Features and Prognosis in Invasive Ductal Carcinoma of the Breast[J].Journal of Medical Information,2020,33(17):68.[doi:10.3969/j.issn.1006-1959.2020.01.022]
[3]周 涛.Ⅱ、Ⅲ期直肠癌Ki-67及微卫星不稳定的表达对预后的影响[J].医学信息,2021,34(23):66.[doi:10.3969/j.issn.1006-1959.2021.23.018]
 ZHOU Tao.Effect of Ki-67 and Microsatellite Instability on Prognosis in Stage Ⅱ and Ⅲ Rectal Cancer[J].Journal of Medical Information,2021,34(17):66.[doi:10.3969/j.issn.1006-1959.2021.23.018]
[4]王 露,陈思敏,赵苏苏,等.子宫内膜样子宫内膜癌中错配修复基因表达与临床病理及相关基因表达的关系[J].医学信息,2020,33(14):61.[doi:10.3969/j.issn.1006-1959.2020.14.019]
 WANG Lu,CHEN Si-min,ZHAO Su-su,et al.Relationship Between Mismatch Repair Gene Expression and Clinicopathology and Related Gene Expression in Endometrioid Endometrial Carcinoma[J].Journal of Medical Information,2020,33(17):61.[doi:10.3969/j.issn.1006-1959.2020.14.019]
[5]彭小飞,张 涵,廖家李,等.脂联素在乳腺癌组织中的表达及其临床意义[J].医学信息,2021,34(06):6.[doi:10.3969/j.issn.1006-1959.2021.06.002]
 PENG Xiao-fei,ZHANG Han,LIAO Jia-li,et al.The Expression of Adiponectin in Breast Cancer Tissues and Its Clinical Significance[J].Journal of Medical Information,2021,34(17):6.[doi:10.3969/j.issn.1006-1959.2021.06.002]

更新日期/Last Update: 1900-01-01