[1]段达荣,蔡莎莎,林芯伊.假基因POU5F1B在结直肠癌组织中的表达及其临床意义[J].医学信息,2022,35(17):41-44.[doi:10.3969/j.issn.1006-1959.2022.17.009]
　DUAN Da-rong,CAI Sha-sha,LIN Xin-yi.Expression of Pseudogene POU5F1B in Colorectal Cancer Tissues and its Clinical Significance[J].Journal of Medical Information,2022,35(17):41-44.[doi:10.3969/j.issn.1006-1959.2022.17.009]
点击复制

假基因POU5F1B在结直肠癌组织中的表达及其临床意义()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年17期
页码:
41-44
栏目:
论著
出版日期:
2022-09-01

文章信息/Info

Title:
Expression of Pseudogene POU5F1B in Colorectal Cancer Tissues and its Clinical Significance
文章编号:
1006-1959（2022）17-0041-04
作者:
段达荣; 蔡莎莎; 林芯伊
（台州市第一人民医院检验科，浙江 台州 318020）
Author(s):
DUAN Da-rong; CAI Sha-sha; LIN Xin-yi
(Department of Clinical Laboratory,the First People’s Hospital of Taizhou,Taizhou 318020,Zhejiang,China)
关键词:
结直肠癌; 转录组测序技术; POU5F1B; 免疫组化
Keywords:
Colorectal cancer; Transcriptome sequencing technology; POU5F1B; Immunohistochemistry
分类号:
R735.34
DOI:
10.3969/j.issn.1006-1959.2022.17.009
文献标志码:
A
摘要:
目的 了解结直肠组织假基因POU5F1B表达水平及临床意义。方法 收集2017年1月-2021年10月台州市第一人民医院收治的结直肠癌患者143例，应用转录组测序技术（RNA-Seq）及免疫组化染色技术分析结直肠癌组织及癌旁正常组织的假基因POU5F1B表达情况。结果 结直肠癌组织有1520个差异基因（上调基因有989个，下调基因有531个），假基因POU5F1B为上调基因，高于其它基因，结直肠癌组织与癌旁组织POU5F1B基因水平比较，差异有统计学意义（P＜0.05）；根据免疫组化染色强度及面积进行计算，癌组织POU5F1B表达为（1.51±0.68），高于癌旁组织的（0.34±0.54），差异有统计学意义（P＜0.05）；免疫组化染色显示癌旁组织细胞胞浆不着色或浅黄色，为不表达或低表达，癌组织细胞胞浆呈棕黄色，占比约为80%，为高表达。结论 假基因POU5F1B在结直肠癌患者肿瘤组织中为上调基因，其在结直肠癌组织呈高表达，且高于癌旁组织。
Abstract:
Objective To investigate the expression of pseudogene POU5F1B in colorectal tissue and its clinical significance.Methods A total of 143 patients with colorectal cancer who were admitted to the First People’s Hospital of Taizhou from January 2017 to October 2021 were enrolled. Transcriptome sequencing (RNA-Seq) and immunohistochemical staining were used to analyze the expression of pseudogene POU5F1B in colorectal cancer tissues and adjacent normal tissues.Results There were 1520 differentially expressed genes in colorectal cancer tissues (989 up-regulated genes and 531 down-regulated genes). The pseudogene POU5F1 B was up-regulated gene, which was higher than other genes. The difference in POU5F1 B gene level between colorectal cancer tissues and adjacent tissues was statistically significant (P<0.05). According to the intensity and area of immunohistochemical staining, the expression of POU5F1B in cancer tissues was (1.51±0.68), which was higher than (0.34±0.54) in adjacent tissues, and the difference was statistically significant (P<0.05). Immunohistochemical staining showed that the cytoplasm of adjacent tissues was not stained or light yellow, which was not expressed or lowly expressed; the cytoplasm of cancer tissues was brownish yellow, accounting for about 80%, which was highly expressed.Conclusion The pseudogene POU5F1B is an up-regulated gene in tumor tissues of colorectal cancer patients, which is highly expressed in colorectal cancer tissues and higher than that in adjacent tissues.

参考文献/References:

[1]Edwards TL,Shrubsole MJ,Cai Q,et al.Genome-wide association study identifies possible genetic risk factors for colorectal adenomas[J].Cancer Epidemiol Biomarkers Prev,2013,22(7):1219-1226.[2]李群,李逸群,张弘纲,等.122例结直肠癌卵巢转移患者的临床病理特征及预后[J],中华肿瘤杂志,2021,43(1):132-136.[3]Bray F,Ferlay J,Soerjomataram I,et al.Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J].CA Cancer Journal for Clinicians,2018,68(6):394-424.[4]Boeckx N,Koukakis R,Op de Beeck K,et al.Primary tumor sidedness has an impact on prognosis and treatment outcome in metastatic colorectal cancer: results from two randomized first-line panitumumab studies[J].Ann Oncol,2017,28(8):1862-1868.[5]国家卫生和计划生育委员会《中国结直肠癌诊疗规范(2017年版)》专家组.中国结直肠癌诊疗规范(2017年版)[J].中华临床医师杂志(电子版),2018,12(1):3-23.[6]World Health Organizaion.Media centre: cancer[EB/OL].http://www.who.int/mediacentre/factsheets/fs297/en,2018-02-01/2021-12-01.[7]Chen WQ,Zheng RS,Zhang SW,et al.Cancer incidence and mortality in china in 2013:an analysis based on urbanization level [J].Chin J Cancer Res,2017,29(1):1-10.[8]Watanabe T,Muro K,Ajioka Y,et al.Japanese Society for Cancer of the Colon and Rectum (JSCCR) guidelines 2016 for the treatment of colorectal cancer[J].Int J Clin Oncol,2018,23(1):1-34.[9]Vermeer NCA,Backes Y,Snijders HS,et al.National cohort study on postoperative risks after surgery for submucosal invasive colorectal cancer[J].BJS Open,2019,3(2):210-217.[10]庄奥博,朱德祥,许平平,等.T1期结直肠癌淋巴结转移的危险因素分析及其列线图预测模型的应用价值[J].中华消化外科杂志,2021,20(3):323-330.[11]Saitoh Y,Inaba Y,Sasaki T,et al.Management of colorectal T1 carcinoma treated by endoscopic resection[J].Dig Endosc,2016,28(3):324-329.[12]Hayashi H,Arao T,Togashi Y,et al.The OCT4 pseudogene POU5F1B is amplified and promotes an aggressive phenotype in gastric cancer[J].Oncogene,2015,34(2):199-208.[13]Ji H,Greening DW,Barnes TW,et al.Proteome profiling of exosomes derived from human primary and metastatic colorectal cancer cells reveal differential expression of key metastatic factors and signal transduction components[J].Proteomics,2013,13(10-11):1672-1686.[14]余菁雯,朱萍,周留林.假基因POU5F1B对宫颈癌SiHa细胞增殖、迁移和侵袭能力的影响[J].临床肿瘤学杂志,2019,24(7):577-583.[15]Small W Jr,Bacon MA,Bajaj A,et al.Cervical cancer:A global health crisis[J].Cancer,2017,123(13):2404-2412.[16]Su YR,Di C,Bien S,et al.A Mixed-Effects Model for Powerful Association Tests in Integrative Functional Genomics[J].American Journal of Human Genetics,2018,102(5):904-919.[17]Zhu P,Wu J,Wang Y,et al.LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis[J].Nat Cell Biol,2018,20(10):1134-1144.[18]Villodre ES,Kipper FC,Pereira MB,et al.Roles of OCT4 in tumorigenesis,cancer therapy resistance and prognosis[J].Cancer Treat Rev,2016,51:1-9.[19]Le TD,Zhang J,Liu L,et al.Computational methods for identifying miRNA sponge interactions[J].Brief Bioinform, 2017,18(4):577-590.[20]Pan Y,Zhan L,Chen L,et al.POU5F1B promotes hepatocellular carcinoma proliferation by activating AKT[J].Biomed Pharmacother,2018,100:374-380.

相似文献/References:

[1]张西园.糖尿病与结直肠癌患者预后相关研究进展[J].医学信息,2018,31(01):55.[doi:10.3969/j.issn.1006-1959.2018.01.020]
　ZHANG Xi-yuan.Research Progress in the Relationship between Diabetes and the Prognosis of Colorectal Cancer[J].Journal of Medical Information,2018,31(17):55.[doi:10.3969/j.issn.1006-1959.2018.01.020]
[2]陈学敏,彭 琼.中性粒细胞与淋巴细胞比值联合癌胚抗原对结直肠癌早期诊断的研究[J].医学信息,2019,32(02):75.[doi:10.3969/j.issn.1006-1959.2019.02.022]
　CHEN Xue-min,PENG Qiong.Early Diagnosis of Colorectal Cancer by Neutrophil-lymphocyte Ratio Combined with Carcinoembryonic Antigen[J].Journal of Medical Information,2019,32(17):75.[doi:10.3969/j.issn.1006-1959.2019.02.022]
[3]李在明,魏正强.外泌体在结直肠癌中的研究进展[J].医学信息,2019,32(03):42.[doi:10.3969/j.issn.1006-1959.2019.03.014]
　LI Zai-ming,WEI Zheng-qiang.Research Progress of Exosomes in Colorectal Cancer[J].Journal of Medical Information,2019,32(17):42.[doi:10.3969/j.issn.1006-1959.2019.03.014]
[4]周玲玲,鞠乐乐,颜 玉.结直肠癌患者手术前后血清IGF-1水平变化及临床价值分析[J].医学信息,2019,32(04):121.[doi:10.3969/j.issn.1006-1959.2019.04.039]
　ZHOU Ling-ling,JU Le-le,YAN Yu.Analysis of Changes and Clinical Value of Serum IGF-1 Levels in Patients with Colorectal Cancer before and after Operation[J].Journal of Medical Information,2019,32(17):121.[doi:10.3969/j.issn.1006-1959.2019.04.039]
[5]陈小玉,白 鍊,贺春香.结直肠癌根治手术患者营养状况调查[J].医学信息,2019,32(05):83.[doi:10.3969/j.issn.1006-1959.2019.05.025]
　CHEN Xiao-yu,BAI Lian,HE Chun-xiang.Investigation on Nutritional Status of Patients Undergoing Radical Surgery for Colorectal Cancer[J].Journal of Medical Information,2019,32(17):83.[doi:10.3969/j.issn.1006-1959.2019.05.025]
[6]李大略,赵资文,刘兆礼,等.加速康复外科对合并有糖尿病的结直肠癌患者术后炎症反应与营养代谢的影响[J].医学信息,2019,32(07):67.[doi:10.3969/j.issn.1006-1959.2019.07.021]
　LI Da-lue,ZHAO Zi-wen,LIU Zhao-li,et al.Effect of Accelerated Rehabilitation Surgery on Postoperative Inflammatory Response and Nutrient Metabolism in Patients with Colorectal Cancer Complicated with Diabetes[J].Journal of Medical Information,2019,32(17):67.[doi:10.3969/j.issn.1006-1959.2019.07.021]
[7]王逸飞,王 志,左宏波,等.伊利替康联合替吉奥在晚期结直肠癌治疗的临床研究[J].医学信息,2019,32(07):158.[doi:10.3969/j.issn.1006-1959.2019.07.049]
　WANG Yi-fei,WANG Zhi,ZUO Hong-bo,et al.Clinical Study of Irinotecan Combined with Tegafur,Gimeracil and Oteracil Porassium Capsulesin the Treatment of Advanced Colorectal Cancer[J].Journal of Medical Information,2019,32(17):158.[doi:10.3969/j.issn.1006-1959.2019.07.049]
[8]谢 勇,涂经楷,肖明盛,等.腹腔镜与传统开腹结直肠癌根治术手术效果对比[J].医学信息,2019,32(11):98.[doi:10.3969/j.issn.1006-1959.2019.11.026]
　XIE Yong,TU Jing-kai,XIAO Ming-sheng,et al.Comparative of Laparoscopic and Traditional Open Radical Rectal Cancer Radical Operation[J].Journal of Medical Information,2019,32(17):98.[doi:10.3969/j.issn.1006-1959.2019.11.026]
[9]符青云,欧阳小明,郅 程,等.结直肠癌组织EGFR、BRAF及NRAS基因突变检测及临床意义分析[J].医学信息,2022,35(13):83.[doi:10.3969/j.issn.1006-1959.2022.13.018]
　FU Qing-yun,OUYANG Xiao-ming,ZHI Cheng,et al.Detection and Clinical Significance of EGFR, BRAF and NRAS Gene Mutations in Colorectal Cancer[J].Journal of Medical Information,2022,35(17):83.[doi:10.3969/j.issn.1006-1959.2022.13.018]
[10]李莎莎,李 芳,王卫峰,等.地中海饮食与结直肠癌的相关研究[J].医学信息,2019,32(15):6.[doi:10.3969/j.issn.1006-1959.2019.15.003]
　LI Sha-sha,LI Fang,WANG Wei-feng,et al.Correlation between Mediterranean Diet and Colorectal Cancer[J].Journal of Medical Information,2019,32(17):6.[doi:10.3969/j.issn.1006-1959.2019.15.003]

更新日期/Last Update: 1900-01-01