[1]任默涵,于东海,吴 迪.MTHFD2基因在膀胱癌中的表达、功能富集及信号通路的生物信息学分析[J].医学信息,2022,35(24):18-22.[doi:10.3969/j.issn.1006-1959.2022.24.004]
　REN Mo-han,YU Dong-hai,WU Di.Expression, Functional Enrichment and Bioinformatics Analysis of MTHFD2 Gene in Bladder Cancer[J].Journal of Medical Information,2022,35(24):18-22.[doi:10.3969/j.issn.1006-1959.2022.24.004]
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MTHFD2基因在膀胱癌中的表达、功能富集及信号通路的生物信息学分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年24期
页码:
18-22
栏目:
生物信息学
出版日期:
2022-12-15

文章信息/Info

Title:
Expression, Functional Enrichment and Bioinformatics Analysis of MTHFD2 Gene in Bladder Cancer
文章编号:
1006-1959（2022）24-0018-05
作者:
任默涵; 于东海; 吴 迪
（天津市第三中心医院放射科，天津 300170）
Author(s):
REN Mo-han; YU Dong-hai; WU Di
(Department of Radiology,Tianjin Third Central Hospital,Tianjin 300170,China)
关键词:
膀胱癌; MTHFD2基因; 无病生存期; 淋巴细胞
Keywords:
Bladder cancer; MTHFD2 gene; Disease-free survival; Lymphocytes
分类号:
Q811.4；R737.14
DOI:
10.3969/j.issn.1006-1959.2022.24.004
文献标志码:
A
摘要:
目的 采用数据挖掘技术探讨亚甲基四氢叶酸脱氢酶2基因（MTHFD2）在膀胱癌中的表达、生物学功能、相关信号通路及其与患者预后的关系。方法 在癌症基因组图谱数据库（TCGA）中比较人多种实体肿瘤及膀胱癌组织和正常组织中MTHFD2基因mRNA的相对表达水平，String数据库构建MTHFD2基因相关蛋白-蛋白相互作用网络，对MTHFD2及相关基因功能进行KEGG信号通路功能富集；根据膀胱癌患者癌组织中MTHFD2基因表达中位数分为高、低表达组，绘制风险比例模型，Log-rank检验比较MTHFD2高低表达组患者总生存期（OS）和无病生存期（DFS）有无差异。结果 MTHFD2基因mRNA在人体大多数肿瘤组织中呈现高表达（P＜0.05）；MTHFD2基因mRNA在癌组织中重度表达水平高于癌旁正常组织（P＜0.05），随着MTHFD2基因mRNA表达水平的增高，膀胱癌的分期也增加（P＜0.05）； MTHFD2蛋白-蛋白相互作用网络中共有21个蛋白和47个相互作用，各蛋白平均相互作用指数为0.893，网络中各个蛋白富集明显（P＜0.05）；MTHFD2基因功能主要富集于上皮肿瘤发生、线粒体和甲酰基及相关转移酶活性；MTHFD2基因表达与CD8+T细胞（r=0.364）、巨噬细胞（r=0.145）、中性粒细胞（r=0.269）及树突状细胞（r=0.407）存在相关性；MTHFD2高表达组膀胱癌患者OS（HR=1.5，P=0.000 62）和DFS（HR=1.5，P=0.015）低于低表达组。结论 MTHFD2在膀胱癌患者癌组织中呈高表达水平，其可能参与了膀胱癌的发生与发展，并与患者的预后不良有关。
Abstract:
Objective To investigate the expression, biological function, related signaling pathways and prognosis of methylene tetrahydrofolate dehydrogenase 2 (MTHFD2) in bladder cancer by data mining.Methods The relative expression levels of MTHFD2 gene mRNA in various solid tumors and bladder cancer tissues and normal tissues were compared in the Cancer Genome Atlas ( TCGA ) database. The String database was used to construct the MTHFD2 gene-related protein-protein interaction network, and the KEGG signaling pathway function enrichment of MTHFD2 and related gene functions was performed. According to the median expression of MTHFD2 gene in bladder cancer tissues, the patients were divided into high and low expression groups, and the risk ratio model was drawn. Log-rank test was used to compare the overall survival (OS) and disease-free survival (DFS) of patients with high and low expression of MTHFD2.Results MTHFD2 mRNA was highly expressed in most human tumor tissues (P<0.05). The expression level of MTHFD2 mRNA in cancer tissues was higher than that in adjacent normal tissues (P<0.05). With the increase of MTHFD2 mRNA expression level, the stage of bladder cancer increased (P<0.05). There were 21 proteins and 47 interactions in the MTHFD2 protein-protein interaction network. The average interaction index of each protein was 0.893, and each protein in the network was significantly enriched (P<0.05). The function of MTHFD2 gene is mainly enriched in epithelial tumorigenesis, mitochondria and formyl and related transferase activity. MTHFD2 gene expression was correlated with CD8+ T cells (r=0.364), macrophages (r=0.145), neutrophils (r=0.269) and dendritic cells (r=0.407). The OS (HR=1.5, P=0.000 62) and DFS (HR=1.5, P=0.015) of bladder cancer patients in the MTHFD2 high expression group were lower than those in the low expression group.Conclusion MTHFD2 is highly expressed in bladder cancer tissues, which may be involved in the occurrence and development of bladder cancer and is related to the poor prognosis of patients.

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更新日期/Last Update: 1900-01-01