[1]唐雨帆,宋鹏书,李 英,等.细胞焦亡在胎盘植入患者外周血和胎盘组织中的表达及临床意义[J].医学信息,2024,37(10):83-87.[doi:10.3969/j.issn.1006-1959.2024.10.016]
　TANG Yu-fan,SONG Peng-shu,LI Ying,et al.Expression and Clinical Significance of Pyroptosisin Peripheral Blood and Placental Tissues of Patients with Placenta Implantation[J].Journal of Medical Information,2024,37(10):83-87.[doi:10.3969/j.issn.1006-1959.2024.10.016]
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细胞焦亡在胎盘植入患者外周血和胎盘组织中的表达及临床意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年10期
页码:
83-87
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Expression and Clinical Significance of Pyroptosisin Peripheral Blood and Placental Tissues of Patients with Placenta Implantation
文章编号:
1006-1959（2024）10-0083-05
作者:
唐雨帆; 宋鹏书; 李 英; 等
（广西壮族自治区妇幼保健院新阳产二科，广西 南宁 530000）
Author(s):
TANG Yu-fan; SONG Peng-shu; LI Ying; et al.
(Xinyang Second Department of Obstetrics,Guangxi Zhuang Autonomous Region maternal and Child Health Hospital,Nanning 530003,Guangxi,China)
关键词:
细胞焦亡; 胎盘植入; 炎症因子
Keywords:
Pyroptosis; Placenta implantation; Inflammatory factor
分类号:
R714.252；Q291
DOI:
10.3969/j.issn.1006-1959.2024.10.016
文献标志码:
A
摘要:
目的 探究胎盘植入患者外周血中炎症因子的表达及胎盘组织中细胞焦亡因子的表达情况和临床意义。方法 收集2021年6月-2023年1月在我院产科入院诊断为胎盘植入30例孕妇作为胎盘植入组，妊娠期正常孕妇60例作为正常组，分析两组孕妇的临床资料信息。通过ELISA检测TNF-α、IL-1β及IL-18表达情况，HE染色观察胎盘植入的胎盘组织病理学，IHC和qPCR实验检测Caspase-1、GSDMD和NLRP3蛋白和mRNA在两组孕妇胎盘组织中表达差异。结果 胎盘植入组孕妇外周血中TNF-α、IL-1β和IL-18表达量高于正常组，差异有统计学意义（P＜0.05）；胎盘植入患者的子宫基层组织没有蜕膜，绒毛膜绒毛直接毗邻肌层纤维。胎盘植入组胎盘组织中细胞焦亡分子Caspase-1和NLRP3蛋白和mRNA表达高于正常组，GSDMD蛋白和mRNA表达低于正常组（P＜0.05）。结论 细胞焦亡在胎盘植入发病机制过程中表达增加。
Abstract:
Objective To investigate the expression of inflammatory factors in peripheral blood of patients with placenta implantation and the expression of pyroptosis factors in placental tissues and their clinical significance.Methods From June 2021 to January 2023, 30 pregnant women diagnosed with placenta implantation in the obstetrics department of our hospital were collected as the placenta implantation group, and 60 normal pregnant women during pregnancy were collected as the normal group. The clinical data of the two groups were analyzed. The expression of TNF-α, IL-1β and IL-18 was detected by ELISA. The histopathology of placenta implantation was observed by HE staining. The expression of Caspase-1, GSDMD and NLRP3 protein and mRNA in placenta of two groups was detected by IHC and qPCR.Results The expression levels of TNF-α, IL-1β and IL-18 in peripheral blood of pregnant women in the placenta implantation group were higher than those in the normal group, and the differences were statistically significant (P<0.05). There was no decidua in the uterine basal tissue of patients with placenta implantation, and the chorionic villi were directly adjacent to the myometrium fibers. The expression of Caspase-1 and NLRP3 protein and mRNA in placenta tissue of placenta implantation group was higher than that of normal group, and the expression of GSDMD protein and mRNA was lower than that of normal group (P<0.05).Conclusion The expression of pyroptosis is increased in the pathogenesis of placental implantation.

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更新日期/Last Update: 1900-01-01