[1]尹 江,张志杰,贺智敏.miRNA205在神经胶质瘤细胞放疗敏感性中的作用[J].医学信息,2021,34(13):1-3.[doi:10.3969/j.issn.1006-1959.2021.13.001]
　YIN Jiang,ZHANG Zhi-jie,HE Zhi-min.The Role of miRNA205 in the Sensitivity of Glioma Cells to Radiotherapy[J].Medical Information,2021,34(13):1-3.[doi:10.3969/j.issn.1006-1959.2021.13.001]
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miRNA205在神经胶质瘤细胞放疗敏感性中的作用()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年13期
页码:
1-3
栏目:
出版日期:
2021-07-01

文章信息/Info

Title:
The Role of miRNA205 in the Sensitivity of Glioma Cells to Radiotherapy
文章编号:
1006-1959（2021）13-0001-03
作者:
尹 江; 张志杰; 贺智敏
（广州医科大学附属肿瘤医院肿瘤研究所，广东 广州 510095）
Author(s):
YIN Jiang; ZHANG Zhi-jie; HE Zhi-min
(Institute of Oncology,Affiliated Tumor Hospital of Guangzhou Medical University,Guangzhou 510095,Guangdong,China)
关键词:
胶质瘤; miRNA205; 放疗敏感性; DNA损伤修复
Keywords:
Glioma; miRNA205; Radiotherapy sensitivity; DNA damage repair
分类号:
R739
DOI:
10.3969/j.issn.1006-1959.2021.13.001
文献标志码:
A
摘要:
目的 观察miRNA205对神经胶质瘤放疗敏感性的影响，并探讨其增敏机制。方法 采用miRNA mimic miRNA205及阴性对照miRNA mimic NC分别转染胶质瘤细胞U251，RT-qPCR检测miRNA205的表达量，平板克隆检测细胞放射治疗后的克隆形成能力及Western Blot检测DNA损伤修复能力。结果 miRNA205mimic能够上调细胞中miRNA205的过表达（11.3±1.55）倍，经6 Gy放射治疗后对照组U251/NC形成的克隆数为（37.33±5.86）个，过表达miRNA205的实验组U251/miR205形成的克隆数为（15±7.94）个；放疗24小时后U251细胞中的DNA损伤标志物恢复到正常水平，而U251/miRNA205在放疗后24 h仍有较高表达。结论 miRNA205能够抑制U251细胞的DNA损伤修复能力，抑制细胞放疗后的克隆形成能力、提高放疗敏感性。
Abstract:
Objective To observe the effect of miRNA205 on the sensitivity of glioma to radiotherapy, and to explore its sensitization mechanism. Methods The glioma cells U251 were transfected with miRNA mimic miRNA205 and the negative control miRNA mimic NC. RT-qPCR was used to detect the expression of miRNA205. The plate clone was used to detect the clone formation ability of the cells after radiotherapy and Western Blot to detect the DNA damage repair ability.Results miRNA205mimic can up-regulate the overexpression of miRNA205 in cells (11.3±1.55) times,after 6 Gy radiotherapy, the number of clones formed by U251/NC in the control group was (37.33±5.86), and the number of clones formed by U251/miR205 in the experimental group overexpressing miRNA205 was (15±7.94);The DNA damage markers in U251 cells returned to normal levels 24 h after radiotherapy,while U251/miRNA205 still had a high expression 24 h after radiotherapy.Conclusion miRNA205 can inhibit the DNA damage repair ability of U251 cells, inhibit the clone formation ability of cells after radiotherapy, and improve the sensitivity of radiotherapy.

参考文献/References:

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更新日期/Last Update: 1900-01-01