[1]NEELAM SAH,聂小凤,张 琴,等.宫颈脱落细胞miR-125b与宫颈病变关系及其在HR-HPV 阳性患者分层筛查中的作用研究[J].医学信息,2018,31(23):76-78,82.[doi:10.3969/j.issn.1006-1959.2018.23.021]
　NEELAM SAH,NIE Xiao-feng,ZHANG Qin,et al.The Relationship between Cervical Exfoliated Cell miR-125b and Cervical Lesions and its Role in Stratified Screening of HR-HPV Positive Patients[J].Journal of Medical Information,2018,31(23):76-78,82.[doi:10.3969/j.issn.1006-1959.2018.23.021]
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宫颈脱落细胞miR-125b与宫颈病变关系及其在HR-HPV 阳性患者分层筛查中的作用研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年23期
页码:
76-78,82
栏目:
论著
出版日期:
2018-12-01

文章信息/Info

Title:
The Relationship between Cervical Exfoliated Cell miR-125b and Cervical Lesions and its Role in Stratified Screening of HR-HPV Positive Patients
文章编号:
1006-1959（2018）23-0076-04
作者:
NEELAM SAH; 聂小凤; 张 琴; 张素仙; 杨丽华
昆明医科大学第二附属医院妇科，云南 昆明 650101
Author(s):
NEELAM SAH; NIE Xiao-feng; ZHANG Qin; ZHANG Su-xian; YANG Li-hua
Department of Gynecology,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,Yunnan,China
关键词:
宫颈病变; miR-125b; HR-HPV; 宫颈癌筛查
Keywords:
Cervical lesions; miR-125b; HR-HPV; Cervical cancer screening
分类号:
RR737.33
DOI:
10.3969/j.issn.1006-1959.2018.23.021
文献标志码:
A
摘要:
比较正常宫颈、宫颈上皮内瘤变及宫颈癌患者宫颈脱落细胞中miR-125b表达水平差异，探讨宫颈脱落细胞miR-125b表达水平检测在HR-HPV阳性患者分层筛查中的作用及其与宫颈病变的关系。方法 采用第二代杂交捕获技术进行HR-HPV初筛，HR-HPV阳性患者行宫颈液基薄层细胞学检查及miR-125b检测。液基细胞学检查结果异常者直接阴道镜下活检行病理学检查，液基细胞学检查正常者行阴道镜检查，阴道镜检查下发现异常者行阴道镜下活检取材，余视作宫颈组织学正常，不做宫颈活检。RT-qPCR检测正常宫颈及不同级别宫颈病变中miR-125b表达水平，比较不同组织学分组之间的宫颈脱落细胞miR-125b表达水平，绘制ROC曲线，探讨miR-125b、液基细胞学检查在宫颈病变中的诊断作用。结果 宫颈脱落细胞miR-125b水平随着宫颈病变程度加重逐渐升高（P＜0.05）；在HR-HPV阳性患者分层筛查中，宫颈液基细胞学诊断宫颈高级别上皮内病变的敏感性、特异性分别为64.90%、80.70%，AUC为0.728（95%CI：0.657~0.779，P＜0.001）。与液基细胞学检查相比，宫颈脱落细胞miR-125b检测诊断宫颈高级别上皮内病变的灵敏性、特异性更高（87.30% vs 64.90%；69.20% vs 80.70%），AUC为 0.864（95%CI：0.800~0.928，P＜0.001）。结论 miR-125b水平随着宫颈病变加重逐渐升高，检测宫颈脱落细胞miR-125b水平可有效分流HR-HPV阳性患者，敏感性高于细胞学检查，可能成为一种有效的分层筛查方法。
Abstract:
Objective To compare the expression levels of miR-125b in cervical exfoliated cells of normal cervix, cervical intraepithelial neoplasia and cervical cancer, and to explore the role of miR-125b expression in cervical exfoliated cells in stratified screening of HR-HPV positive patients. The relationship between cervical lesions. Methods The second-generation hybridization capture technique was used for HR-HPV screening. The HR-HPV-positive patients underwent cervical-based thin-layer cytology and miRNA125b detection. The results of liquid-based cytology were abnormal. The pathological examination was performed directly under biopsy. The liquid-based cytology was performed by colposcopy. The abnormalities were found under colposcopy. The colposcopy biopsy was performed. Normal, no cervical biopsy. RT-qPCR was used to detect the expression of miR-125b in normal cervical and different grades of cervical lesions. The expression levels of miR-125b in cervical exfoliated cells were compared between different histological groups. ROC curves were drawn to investigate miR-125b and liquid-based cytology. The role of diagnosis in cervical lesions. Results The level of miR-125b in cervical exfoliated cells increased with the severity of cervical lesions (P<0.05). In the stratified screening of HR-HPV positive patients, the sensitivity of cervical liquid-based cytology in the diagnosis of high-grade intraepithelial lesions of the cervix, The specificities were 64.90% and 80.70%, respectively, and the AUC was 0.728 (95% CI: 0.657~0.779,P<0.001). Compared with liquid-based cytology, the sensitivity and specificity of cervical exfoliated cells miR-125b for the diagnosis of high-grade intraepithelial lesions of the cervix were higher (87.30% vs 64.90%; 69.20% vs 80.70%), and the AUC was 0.864 (95%CI: 0.800~0.928,P<0.001). Conclusion The level of miR-125b is gradually increased with the increase of cervical lesions. The detection of miR-125b in cervical exfoliated cells can effectively divert HR-HPV positive patients, which is more sensitive than cytology and may be an effective stratified screening method.

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更新日期/Last Update: 2018-12-17