[1]杨巧侠,王成果,王元欣.乌司他丁预处理对HepG2细胞氧化损伤保护作用的研究[J].医学信息,2019,32(13):72-76.[doi:10.3969/j.issn.1006-1959.2019.13.020]
　YANG Qiao-xia,WANG Cheng-guo,WANG Yuan-xin.Protective Effect of Ulinastatin Pretreatment on Oxidative Damage in HepG2 Cells[J].Journal of Medical Information,2019,32(13):72-76.[doi:10.3969/j.issn.1006-1959.2019.13.020]
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乌司他丁预处理对HepG2细胞氧化损伤保护作用的研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年13期
页码:
72-76
栏目:
论著
出版日期:
2019-07-01

文章信息/Info

Title:
Protective Effect of Ulinastatin Pretreatment on Oxidative Damage in HepG2 Cells
文章编号:
1006-1959（2019）13-0072-05
作者:
杨巧侠1; 王成果2; 王元欣3
1.扶风县人民医院消化内科，陕西 宝鸡 722200； 2.空军军医大学唐都医院普外科，陕西 西安 710038； 3.扶风县人民医院神经外科，陕西 宝鸡 722200
Author(s):
YANG Qiao-xia1; WANG Cheng-guo2; WANG Yuan-xin3
1.Department of Gastroenterology,Fufeng County People's Hospital,Baoji 722200,Shaanxi,China; 2.Department of genealsurgery,Tangdu Hospital, Air Force Military Medical University,Xi’an,710038,Shaanxi,China; 3.Department of Neurosurgery,Fufeng County People
关键词:
乌司他丁; 自噬; 凋亡; 氧化应激
Keywords:
Key words：Ulinastatin; Autophagy; Apoptosis; Oxidative stress
分类号:
R575.1
DOI:
10.3969/j.issn.1006-1959.2019.13.020
文献标志码:
A
摘要:
目的 探讨乌司他丁（UTI）预处理对HepG2细胞氧化应激损伤的保护作用。方法 将HepG2细胞常规培养传代，随机分为H2O2组，UTI100+H2O2组、UTI500+H2O2组、UTI1000+H2O2组及对照组，其中对照组不作任何处理，H2O2组，UTI100+H2O2组、UTI500+H2O2组、UTI1000+H2O2组分别加入浓度为0 μmol/L、100 μmol/L、500 μmol/L、1000 μmol/L UTI预处理，24 h后加入300 μmol/L H2O2处理4 h，体外模拟肝细胞损伤。CCK-8试剂盒和LDH试剂盒检测LDH变化评价细胞损伤；TUNEL检测细胞凋亡率；Western blot检测HepG2细胞中LC3-Ⅱ、Cleaved caspase-3的表达变化情况。结果 与H2O2组相比较，CCK-8和LDH检测结果提示，各TUI预处理组能改善细胞生存环境，减轻H2O2对HepG2细胞的应激损伤，提高细胞活力（P＜0.05）；TUNEL检测提示预处理可降低氧化应激导致的凋亡率（P＜0.05）； Western blot结果提示，与H2O2组比较，UTI可上调LC3-Ⅱ的表达（P＜0.05），促进细胞自噬的发生，降低Cleaved caspase-3表达，抑制细胞凋亡 (P＜0.05)。结论 UTI可以通过激活LC3-Ⅱ表达，促进细胞自噬，发挥保护作用，拮抗氧化应激导致的细胞凋亡。
Abstract:
Abstract：Objective To investigate the protective effect of ulinastatin (UTI) preconditioning on oxidative stress injury in HepG2 cells.Methods HepG2 cells were routinely cultured and randomly divided into H2O2 group, UTI100+ H2O2 group, UTI500+ H2O2 group, UTI1000+ H2O2 group and control group. The control group did not do any treatment. H2O2 group, UTI100+ H2O2 group, UTI500+ H2O2 group,UTI1000+ H2O2 group was pretreated with 0 μmol/L, 100 μmol/L, 500 μmol/L, and 1000 μmol/L UTI. After 24 h, 300 μmol/L H2O2 was added for 4 h to simulate hepatocytes in vitro. damage. CCK-8 kit and LDH kit were used to detect LDH changes to evaluate cell damage; TUNEL was used to detect apoptosis rate; Western blot was used to detect the expression of LC3-II and Cleaved caspase-3 in HepG2 cells. Results Compared with the H2O2 group, the results of CCK-8 and LDH showed that each TUI pretreatment group could improve the cell survival environment, reduce the stress damage of H2O2 on HepG2 cells, and increase cell viability (P<0.05). TUNEL detection suggested pretreatment. The apoptosis rate induced by oxidative stress was reduced (P<0.05).Western blot results showed that compared with H2O2 group, UTI could up-regulate the expression of LC3-II (P<0.05), promote the occurrence of autophagy, decrease the expression of Cleaved caspase-3, and inhibit cell apoptosis (P<0.05). Conclusion UTI can promote the autophagy of cells by activating LC3-II expression, and play a protective role in antagonizing apoptosis induced by oxidative stress.

参考文献/References:

[1]李白雪,张技,吴疆,等.体外肝细胞损伤模型分子机制探讨[J].现代生物医学进展,2014(9):1786-1789. [2]Leng Y.Ulinastatin for acute lung injury and acute respiratory distress syndrome: A systematic review and meta-analysis[J].World Journal of Critical Care Medicine,2014,3(1):34. [3]金明,王玉娇,金梅花,等.两种细胞建立肝细胞氧化损伤模型比较[J].中国公共卫生,2015(3):324-326. [4]Jaeschke H,Ramachandran A.Reactive oxygen species in the normal and acutely injured liver[J]. Journal of Hepatology,2011,55(1):227-228. [5]Guan L.Mechanisms of hepatic ischemia-reperfusion injury and protective effects of nitric oxide[J].World Journal of Gastrointestinal Surgery,2014,6(7):122. [6]Sutter AG,Palanisamy AP,Justin D,et al.Intereukin-10 and Kupffer cells protect steatotic mice livers from ischemia-reperfusion injury[J].European Cytokine Network,2014,25(4):69-76. [7]Zhao G,Zhu Y,Yu D,et al.The effect of ulinastatin on hyperglycemia in patients undergoing hepatectomy[J].Journal of Surgical Research,2015,193(1):223. [8]Luo GJ,Yao WF,He Y,et al.Ulinastatin prevents acute lung injury led by liver transplantation[J].Journal of Surgical Research,2015,193(2):841-848. [9]孙文栋,徐诗雄,陈实.乌司他丁和奥曲肽联合治疗重症急性胰腺炎的疗效及血流变学观察[J].中国生化药物杂志,2014(5):113-115. [10]饶维,张磊,曹宝萍,等.不同剂量乌司他丁预处理对小鼠创伤性脑水肿的影响[J].中华神经外科疾病研究杂志,2012(2):113-116. [11]曹宝萍,张磊,苏宁,等.乌司他丁对创伤性脑损伤的保护作用研究[J].中华神经外科疾病研究杂志,2013(3):244-246. [12]李兵,胡世颉,王冰,等.重度颅脑损伤合并多器官功能衰竭应用乌司他丁治疗效果的临床研究[J].陕西医学杂志,2015(12):1650-1652. [13]Cui T,Zhu G.Ulinastatin attenuates brain edema after traumatic brain injury in rats[J].Cell Biochem Biophys,2015,71(2):595-600. [14]钟毓杰,刘忠民,黄勇平,等.乌司他丁联合粉防己碱预处理对大鼠肝缺血再灌注损伤的保护作用及机制研究[J].肝胆胰外科杂志,2017,29(02):134-137. [15]Lin JD,Cai Y,Xiao XJ,et al.Effect of ulinastatin preconditioning on gene expression profile of heart tissue in a rat sepsis model[J].Chinese Critical Care Mediccine,2010,22(9):547-552. [16]王元欣,张磊,岳康异,等.SIRT3在氧糖剥夺再灌注损伤小鼠神经元中的表达及意义[J].现代生物医学进展,2018(5):817-821. [17]Lavrik IN,Golks A,Krammer PH.Caspases:pharmacological manipulation of cell death[J].J Clin Invest,2005,115(10):2665-2672. [18]Chu VC,Bhattacharya S,Nomoto A,et al.Persistent Expression of Hepatitis C Virus Non-Structural Proteins Leads to Increased Autophagy and Mitochondrial Injury in Human Hepatoma Cells[J].PLoS One,2011(6):e2855112. [19]Rautou PE,Cazals-Hatem D,Moreau R,et al.Acute liver cell damage in patients with anorexia nervosa:a possible role of starvation-induced hepatocyte autophagy[J]. Gastroenterology,2008,135(3):840-848. [20]Simon HU,Haj-Yehia A,Levi-Schaffer F.Role of reactive oxygen species(ROS)in apoptosis induction[J].Apoptosis,2000,5(5):415-418.

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更新日期/Last Update: 2019-07-01