[1]张芦燕,杨 洁,钱一鑫,等.基于HPLC法和指纹图谱的桑菊感冒片质量评价研究[J].医学信息,2023,36(07):139-142.[doi:10.3969/j.issn.1006-1959.2023.07.027]
　ZHANG Lu-yan,YANG Jie,QIAN Yi-xin,et al.Study on Quality Evaluation of Sangju Ganmao tablets Based on HPLC and Fingerprint[J].Journal of Medical Information,2023,36(07):139-142.[doi:10.3969/j.issn.1006-1959.2023.07.027]
点击复制

基于HPLC法和指纹图谱的桑菊感冒片质量评价研究()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年07期
页码:
139-142
栏目:
论著
出版日期:
2023-04-01

文章信息/Info

Title:
Study on Quality Evaluation of Sangju Ganmao tablets Based on HPLC and Fingerprint
文章编号:
1006-1959（2023）07-0139-04
作者:
张芦燕; 杨 洁; 钱一鑫; 等
（1.宁夏药品检验研究院，宁夏 银川 750004；2.贵州大学西南特色药用生物资源开发利用教育部工程研究中心，贵州 贵阳 550025）
Author(s):
ZHANG Lu-yan; YANG Jie; QIAN Yi-xin; et al.
(1.Ningxia Institute for Drug Control,Ningxia 750004,Yinchuan,China;2.Engineering Research Center of the Utilization for Characteristic Bio-Pharmaceutical Resources in Southwest,Ministry of Education,University of Guizhou,Guiyang 550025,Guizhou,China)
关键词:
桑菊感冒片; 高效液相色谱法; 指纹图谱; 相似度
Keywords:
Sangju Ganmao tablets; HPLC; Fingerprint; Simililarity
分类号:
R284
DOI:
10.3969/j.issn.1006-1959.2023.07.027
文献标志码:
A
摘要:
目的 建立不同厂家桑菊感冒片的HPLC指纹图谱，为科学评价桑菊感冒片的质量提供理论依据。方法 采用phenomenex Gemini C18（4.6 mm×250 mm，5 μm）为色谱柱，乙腈-水梯度洗脱程序，检测波长为348 nm，柱温为25 ℃，流速为1.0 ml/min，建立桑菊感冒片的HPLC指纹图谱，使用中药色谱指纹图谱相似度评价系统（2008版）计算相似度，通过对照品比对及文献检索对共有峰进行指认，确定其来源。结果 测定10批次桑菊感冒片的指纹图谱，确立的指纹图谱上有13个共有峰，10批次样品的指纹图谱相似度结果在0.940~0.989，相似度良好；方法学验证结果表明重复性、稳定性、精密度结果均符合要求。结论 本方法操作简单、可靠，适用于桑菊感冒片指纹图谱的建立，可为桑菊感冒片的质量控制提供依据。
Abstract:
Objective To establish an HPLC fingerprint analysis method for Sangju Ganmao tablets of different manufacturers, and to provide a theoretical basis for scientific evaluation of the quality of Sangju Ganmao tablets.Methods The phenomenex Gemini C18 (4.6 mm×250 mm, 5 μm) was used as the chromatographic column, and the acetonitrile-water gradient elution program was used. The detection wavelength was 348 nm, the column temperature was 25 ℃, and the flow rate was 1.0 ml/min. The HPLC fingerprint of Sangju Ganmao tablets was established. The similarity was calculated by the similarity evaluation system chromatographic fingerprint of TCM(Version2008). The common peaks were identified by reference substance comparison and literature search to determine their source.Results There were 13 common peaks on the fingerprints after analyzing chromatograms from 10 batches of Sangju Ganmao tablets. The similarity of 10 batches of samples ranged from 0.940 to 0.989, and the similarity was good. The results of methodological verification showed that the repeatability, stability and precision met the requirements.Conclusion The method is simple, reliable and suitable for the establishment of fingerprint of Sangju Ganmao tablets, which can provide a basis for the quality control of Sangju Ganmao tablets.

参考文献/References:

[1]国家药典委员会.中华人民共和国药典2015年版（一部）[S].北京:中国医药科技出版社,2015.[2]程杰,张屏,包保全,等.肝肾颗粒的制备、质量标准及指纹图谱研究[J].中国药房,2019,30(21):2913-2919.[3]魏丹丹,常相伟,郭盛,等.菊花及菊资源开发利用及资源价值发现策略[J].中国现代中药,2019,21(1):37-44.[4]周明月,霍金海,孙国东,等.采用UPLC-Q-TOF-MS技术鉴定连翘中45中化学成分[J].中国药房,2019,30(22):3067-3073. [5]姚长良,张建青,毕启瑞,等.中药质量标准和检测技术研究及应用[J].中国食品药品监管,2021(9):106-115.[6]刘晶晶,何轶,胡晓茹,等.白癜风胶囊HPLC指纹图谱[J].中国药学杂志,2020,55(1):62-66.[7]李显会,邓美凤,肖伟,等.腰痺通胶囊的高效液相指纹图谱研究[J].沈阳药科大学,2020,37(5):429-432.[8]齐元春,郑艳春,褚莉,等.益心舒片HPLC-ELSD指纹图谱[J].中成药,2020,42(3):802-805.[9]邓梦娇,俞静静,王昕妍,等.HPLC同时测定银菊浸膏中绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸含量[J].中国现代应用药学,2016,33(4):403-407. [10]崔爱瑅,王萌萌,贾首时,等.HPLC-MS/MS法测定桑菊感冒片中5个成分的含量[J].药物分析杂志,2018,38(7):1254-1261.[11]谢淑桐,高晓霞,宋粉云,等. HPLC法同时测定六合定中丸中6种成分[J].中成药,2017,39(5):960-964. [12]胡丹,尹明,曹红,等.舒肝和胃丸质量标准研究[J].药学实践杂志,2019,37(6):532-536.[13]宋新华,陈旭姣,邓冯昕,等.肾衰宁颗粒指纹图谱研究及3种有效成分含量测定[J].药学实践杂志,2020,38(3):259-263.

相似文献/References:

[1]罗 嫄,胡泽锴.高效液相色谱法测定呋塞米片的含量及含量均匀度[J].医学信息,2022,35(09):19.[doi:10.3969/j.issn.1006-1959.2022.09.005]
　LUO Yuan,HU Ze-kai.HPLC Determination of Furosemide Tablets and its Content Uniformity[J].Journal of Medical Information,2022,35(07):19.[doi:10.3969/j.issn.1006-1959.2022.09.005]
[2]赵 杰.HPLC法测定注射用泮托拉唑钠中EDTA-2Na的含量[J].医学信息,2018,31(11):138.[doi:10.3969/j.issn.1006-1959.2018.11.044]
　ZHAO Jie.Determination of EDTA-2Na in Pantoprazole Sodium for Injection by HPLC[J].Journal of Medical Information,2018,31(07):138.[doi:10.3969/j.issn.1006-1959.2018.11.044]
[3]张丽琴,梅 群,肖植国,等.克拉霉素片溶出度检测方法分析[J].医学信息,2018,31(16):126.[doi:10.3969/j.issn.1006-1959.2018.16.039]
　ZHANG Li-qin,MEI Qun,XIAO Zhi-guo,et al.Analysis of Dissolution Test of Clarithromycin Tablets[J].Journal of Medical Information,2018,31(07):126.[doi:10.3969/j.issn.1006-1959.2018.16.039]
[4]荀秀彪,张顺平.高效液相色谱法测定盐酸依托必利片含量的不确定度分析[J].医学信息,2020,33(04):181.[doi:10.3969/j.issn.1006-1959.2020.04.062]
　XUN Xiu-biao,ZHANG Shun-ping.Analysis of Uncertainty in Determination of Etopril Hydrochloride Tablets by High Performance Liquid Chromatography[J].Journal of Medical Information,2020,33(07):181.[doi:10.3969/j.issn.1006-1959.2020.04.062]
[5]荀秀彪,王月德.HPLC法测定银菊感冒胶囊中绿原酸的含量[J].医学信息,2020,33(09):155.[doi:10.3969/j.issn.1006-1959.2020.09.052]
　XUN Xiu-biao,WANG Yue-de.Determination of Chlorogenic Acid in Yinju Ganmao Capsules by HPLC[J].Journal of Medical Information,2020,33(07):155.[doi:10.3969/j.issn.1006-1959.2020.09.052]
[6]许 庆,荀秀彪,肖植国.RP-HPLC法测定栀榆洗剂中栀子苷含量[J].医学信息,2020,33(14):177.[doi:10.3969/j.issn.1006-1959.2020.14.059]
　XU Qing,XUN Xiu-biao,XIAO Zhi-guo.Determination of Jasminoidin in Zhiyu Lotion by RP-HPLC[J].Journal of Medical Information,2020,33(07):177.[doi:10.3969/j.issn.1006-1959.2020.14.059]
[7]童湘伟,石坤连,梁业婷,等.三七不同来源指纹图谱数字化研究[J].医学信息,2021,34(14):106.[doi:10.3969/j.issn.1006-1959.2021.14.028]
　TONG Xiang-wei,SHI Kun-lian,LIANG Ye-ting,et al.Research on Digitization of Fingerprints from Different Sources of Panax Notoginseng[J].Journal of Medical Information,2021,34(07):106.[doi:10.3969/j.issn.1006-1959.2021.14.028]
[8]覃媛媛,沈嘉华.HPLC法测定复方大红袍止血胶囊中没食子酸的含量[J].医学信息,2021,34(16):98.[doi:10.3969/j.issn.1006-1959.2021.16.026]
　QIN Yuan-yuan,SHEN Jia-hua.Determination of Gallic Acid in Compound Dahongpao Zhixue Capsules by HPLC[J].Journal of Medical Information,2021,34(07):98.[doi:10.3969/j.issn.1006-1959.2021.16.026]
[9]蔡锦雄,江 坤,陈颜清,等.HPLC法测定复方氨酚美沙糖浆中对氨基酚含量[J].医学信息,2021,34(16):166.[doi:10.3969/j.issn.1006-1959.2021.16.047]
　CAI Jin-xiong,JIANG Kun,CHEN Yan-qing,et al.Determination of Para-aminophenol in Compound Paracetamol Metha Syrup by HPLC[J].Journal of Medical Information,2021,34(07):166.[doi:10.3969/j.issn.1006-1959.2021.16.047]

更新日期/Last Update: 1900-01-01