[1]施雨南,陈志平,肖秋香,等.KAI1、Snail、Slug、E-cadherin在鼻咽癌中的表达及其与患者预后的关系[J].医学信息,2022,35(11):77-83.[doi:10.3969/j.issn.1006-1959.2022.11.021]
　SHI Yu-nan,CHEN Zhi-ping,XIAO Qiu-xiang,et al.The Expression of KAI1, Snail, Slug, and E-cadherin in Nasopharyngeal Carcinoma and its Relationship with the Prognosis of patients[J].Medical Information,2022,35(11):77-83.[doi:10.3969/j.issn.1006-1959.2022.11.021]
点击复制

KAI1、Snail、Slug、E-cadherin在鼻咽癌中的表达及其与患者预后的关系()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年11期
页码:
77-83
栏目:
论著
出版日期:
2022-06-01

文章信息/Info

Title:
The Expression of KAI1, Snail, Slug, and E-cadherin in Nasopharyngeal Carcinoma and its Relationship with the Prognosis of patients
文章编号:
1006-1959（2022）11-0077-07
作者:
施雨南; 陈志平; 肖秋香; 等
（1.赣南医学院第一临床医学院，江西 赣州 341000；2.赣南医学院第一附属医院检验科，江西 赣州 341000；3.赣南医学院第一附属医院病理科，江西 赣州 341000；4.赣南医学院第一附属医院肿瘤科，江西 赣州 341000）
Author(s):
SHI Yu-nan; CHEN Zhi-ping; XIAO Qiu-xiang; et al.
(1.The First Clinical Medical College,Gannan Medical University,Ganzhou 341000,Jiangxi,China;2.Department of Laboratory Medicine,the First Affiliated Hospital of Gannan Medical University,Ganzhou 341000,Jiangxi,China;3.Department of Pathology,the First
关键词:
鼻咽癌; KAI1; Snail; Slug; E-cadherin; 预后; 转移
Keywords:
Nasopharyngeal carcinoma; KAI1; Snail; Slug; E-cadherin; Prognosis; Metastasis
分类号:
R739.63
DOI:
10.3969/j.issn.1006-1959.2022.11.021
文献标志码:
A
摘要:
目的 研究KAI1和Snail、Slug、E-cadherin在鼻咽癌中的表达情况，分析其与患者预后的关系。方法 收集2011年6月-2015年7月赣南医学院第一附属医院病理科存档石蜡包埋的212例鼻咽癌组织标本，50例鼻咽部黏膜炎组织标本，正常鼻咽上皮组织标本40例，采用免疫组化方法检测各标本中KAI1和Snail、Slug、E-cadherin的表达，通过Kaplan-Meier法评价KAI1和Snail、Slug、E-cadherin的表达与鼻咽癌患者预后的关系，采用Cox回归分析患者临床特征对其生存的影响。结果 KAI1和Snail、Slug、E-cadherin在鼻咽癌标本中的表达阳性率分别为25.94%、65.09%、65.09%和31.60%；鼻咽癌组织中Snail、Slug表达阳性率高于鼻咽部黏膜炎组织和正常鼻咽上皮组织（P＜0.05）；鼻咽癌组织中KAI1、E-cadherin的表达阳性率低于鼻咽部黏膜炎组织和正常鼻咽上皮组织（P＜0.05）；KAI1与Snail（r=-0.435）、Slug（r=-0.473）呈负相关，与E-cadherin呈正相关（r=0.325）；Kaplan-Meier分析显示，Snail（+）和Slug（+）患者的总生存时间短于Snail（-）和Slug（-）患者，KAI1（+）和E-cadherin（+）患者的总生存期长于KAI1（-）和E-cadherin（-）患者（P＜0.05）；Cox回归分析发现，KAI1、Snail、Slug、E-cadherin、淋巴结转移、远处转移、TNM分期是影响鼻咽癌患者预后的独立因素。结论 KAI1和Snail、Slug、E-cadherin的表达及EB-DNA水平、肿瘤大小、淋巴结转移、远处转移、TNM分期与鼻咽癌预后相关，联合检测可辅助判断鼻咽癌患者的预后。
Abstract:
Objective To study the expression of KAI1, Snail, Slug, and E-cadherin in nasopharyngeal carcinoma, and to analyze their relationship with the prognosis of patients.Methods From June 2011 to July 2015, 212 paraffin-embedded specimens of nasopharyngeal carcinoma, 50 specimens of chronic nasopharynx mucositis and 40 specimens of normal nasopharynx epithelium were collected from the Department of Pathology of the First Affiliated Hospital of Gannan Medical College. The expressions of KAI1, Snail, Slug and E-cadherin in each specimen were detected by immunohistochemistry. The relationship between the expressions of KAI1, Snail, Slug and E-cadherin and the prognosis of patients with nasopharyngeal carcinoma was evaluated by Kaplan-Meier method. Cox regression was used to analyze the influence of clinical characteristics of patients on their survival.Results The positive expression rates of KAI1, Snail, Slug and E-cadherin in nasopharyngeal carcinoma specimens were 25.94%, 65.09%, 65.09% and 31.60%, respectively. The positive rates of Snail and Slug expression in nasopharyngeal carcinoma specimens were higher than those in chronic pharyngitis specimens and normal nasopharyngeal mucosa specimens (P<0.05). The positive expression rates of KAI1 and E-cadherin in nasopharyngeal carcinoma specimens were lower than those in chronic nasopharyngitis specimens and normal nasopharyngeal mucosa specimens (P<0.05). KAI1 was negatively correlated with Snail (r=-0.435) and Slug (r=-0.473), and positively correlated with E-cadherin (r=0.325). Kaplan-Meier analysis showed that the overall survival time of Snail(+) and Slug(+) patients was shorter than that of Snail(-) and Slug(-) patients, and the overall survival time of KAI1(+) and E-cadherin(+) patients was longer than that of KAI1(-) and E-cadherin(-) patients (P<0.05). Cox regression analysis showed that KAI1, Snail, Slug, E-cadherin, lymph node metastasis, distant metastasis, and TNM stage were independent factors affecting the prognosis of patients with nasopharyngeal carcinoma.Conclusion The expression of KAI1 and Snail, Slug, E-cadherin is associated with EB-DNA level, tumor size, lymph node metastasis, distant metastasis, TNM stage and prognosis of nasopharyngeal carcinoma. Combined detection can help determine the prognosis of patients with nasopharyngeal carcinoma.

参考文献/References:

[1]Chen YP,Chan ATC,Le QT,et al.Nasopharyngeal carcinoma[J].Lancet,2019,394(10192):64-80.[2]Gioacchini FM,Tulli M,Kaleci S,et al.Prognostic aspects in the treatment of juvenile nasopharyngeal carcinoma: a systematic review[J].Eur Arch Otorhinolaryngol,2017,274(3):1205-1214.[3]Zhang Y,Chen L,Hu GQ,et al.Gemcitabine and Cisplatin Induction Chemotherapy in. Nasopharyngeal Carcinoma[J].N Engl J Med,2019,381(12):1124-1135. [4]Zhu J,Miao C,Liu S,et al.Prognostic role of CD82/KAI1 in multiple human malignant neoplasms: a meta-analysis of 31 studies[J].Onco Targets Ther,2017,10:5805-5816. [5]Wang G,Zhang L,Zhou Y,et al.KAI1/CD82 Genetically Engineered Endothelial Progenitor,Cells Inhibit Metastasis of Human Nasopharyngeal Carcinoma in a Mouse Model[J].Med Sci Monit,2018,24:3146-3152. [6]Singh M,Yelle N,Venugopal C,et al.EMT: Mechanisms and therapeutic implications[J].Pharmacol Ther,2018,182:80-94.[7]Lin X,Chai G,Wu Y,et al.RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail[J].Nat Commun,2019,10(1):2065.[8]Meng J,Ii X,Lei Y,et al.USP5 promotes epithelial-mesenchymal transition by stabilizing SLUG in hepatocellular carcinoma[J].Theranostics,2019,9(2):573-587.[9]Liu H,Ma Y,Heh W,et al.SPHK1 (sphingosine kinase 1) induces epithelial-mesenchymal transition by promoting the autophagy-linked lysosomal degradation of CDH1/E-cadherin in hepatoma cells[J].Autophagy,2017,13(5):900-913.[10]World Medical Association.World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects[J].JAMA,2013,310:2191-2194.[11]Leslie HS,Mary KG,Christian W.TNM Classification of Malignant Tumours[M].New York:NY,2009.[12]Tang Y,Cheng Y,Martinka M,et al.Prognostic significance of KAI1/CD82 in human melanoma and its role in cell migration and invasion through the regulation of ING4[J].Carcinogenesis,2014,35:86-135.[13]Wang M,Wu Q,Jiang Y,et al.The correlation of KAI1, Slug and vasculogenic mimicry in the. prediction of metastasis and prognosis in colorectal carcinoma[J].Int J Clin Exp Pathol,2018,11(10):5034-5043. [14]Chai J,Du L,Ju J,et al.Overexpression of KAI1/CD82 suppresses invitro cell growth, migration, invasion and xenograft growth in oral cancer[J].Mol Med Rep,2017,15(4):1527-1532.[15]Wang G,Jiang H,Xu H,et al.Clinical significance of KAI1/CD82 protein expression in nasopharyngeal carcinoma[J].Oncol Lett,2015,9:1681-1689.[16]Dai C,Liu Y,Yang R,et al.Clinical significance of MACC1, Twist1, and KAI1 expressions in. infiltratingurothelialcarcinoma of the bladder[J].Int J Clin Exp Pathol,2019,12(10):3877-3885.[17]Taki M,Abiko K,Baba T,et al.Snail promotes ovarian cancer progression by recruiting myeloid-derived suppressor cells via CXCR2 ligand upregulation[J].Nat Commun,2018,9(1):1685.[18]Wang Y,Wu Z,Hu L.The regulatory effects of metformin on the [SNAIL/miR-34]:[ZEB/miR-200] system in the epithelial-mesenchymal transition(EMT) for colorectal cancer(CRC)[J].Eur J Pharmacol,2018,834:45-53.[19]Ota I,Masui T,Kurihara M,et al.Snail-induced EMT promotes cancer stem cell-like properties in head and neck cancer cells[J].Oncol Rep,2016,35(1):261-266.[20]Grzegrzolka J,Biala M,Wojtyra P,et al.Expression of EMT Markers SLUG and TWIST in Breast Cancer[J].Anticancer Res,2015,35(7):3961-3968.[21]Krstic M,Kolendowski B,Cecchini MJ,et al.TBX3 promotes progression of pre-invasive breast cancer cells by inducing EMT and directly up-regulating SLUG[J].J Pathol,2019,248(2):191-203.[22]Feldkoren B,Hutchinson R,Rapoport Y,et al.Integrin signaling potentiates transforming growth factor-beta 1 (TGF-1) dependent down-regulation of E-Cadherin expression – Important implications for epithelial to mesenchymal transition (EMT) in renal cell carcinoma[J].Exp Cell Res,2017,355(2):57-66.[23]Cui H,Hu Y,Guo D,et al.DNA methyltransferase 3A isoform b contributes to repressing E-cadherin through cooperation of DNA methylation and H3K27/H3K9 methylation in EMT-related metastasis of gastric cancer[J].Oncogene,2018,37(32):4358-4371.[24]Yao X,Ireland SK,Pham T,et al.TLE1 promotes EMT in A549 lung cancer cells through suppression of E-cadherin[J].Biochem Biophys Res Commun,2014,455(3-4):277-284.

相似文献/References:

[1]林康杰,杨小敏.影响鼻咽癌放疗敏感性相关基因研究进展[J].医学信息,2018,31(22):26.[doi:10.3969/j.issn.1006-1959.2018.22.009]
　LIN Kang-jie,YANG Xiao-min.Advances in Research on Genes Related to Radiotherapy Sensitivity of Nasopharyngeal Carcinoma[J].Medical Information,2018,31(11):26.[doi:10.3969/j.issn.1006-1959.2018.22.009]
[2]龚建忠,韦 燕,潘琦津,等.锌剂防治鼻咽癌同步放化疗患者放射性口咽炎的效果分析[J].医学信息,2019,32(17):63.[doi:10.3969/j.issn.1006-1959.2019.17.020]
　GONG Jian-zhong,WEI Yan,PAN Qi-jin,et al.Effect of Zinc on Prevention and Treatment of Radiation Oropharyngeal Mucositis in Patients with Nasopharyngeal Carcinoma Treated with Concurrent Chemoradiotherapy[J].Medical Information,2019,32(11):63.[doi:10.3969/j.issn.1006-1959.2019.17.020]
[3]潘兴喜,杨 文,李飞龙,等.鼻咽癌咽后淋巴结转移规律对放疗靶区设计的影响[J].医学信息,2019,32(23):107.[doi:10.3969/j.issn.1006-1959.2019.23.029]
　PAN Xing-xi,YANG Wen,LI Fei-long,et al.Effect of Post-Pharyngeal Lymph Node Metastasis on the Design of Radiotherapy Target Area in Nasopharyngeal Carcinoma[J].Medical Information,2019,32(11):107.[doi:10.3969/j.issn.1006-1959.2019.23.029]
[4]周 梅,赵 刚,禤瑞霞,等.鼻咽癌中IKKβ和Ki-67的表达与肿瘤侵袭转移的关系[J].医学信息,2022,35(17):22.[doi:10.3969/j.issn.1006-1959.2022.17.005]
　ZHOU Mei,ZHAO Gang,XUN Rui-xia,et al.Expression of IKKβ and Ki-67 in Nasopharyngeal Carcinoma and its Relationship with Tumor Invasion and Metastasis[J].Medical Information,2022,35(11):22.[doi:10.3969/j.issn.1006-1959.2022.17.005]
[5]杨 柳,余忠华.鼻咽癌放疗所致口腔黏膜反应的防治研究[J].医学信息,2020,33(05):56.[doi:10.3969/j.issn.1006-1959.2020.05.017]
　YANG Liu,YU Zhong-hua.Study on Prevention and Treatment of Oral Mucosa Reaction Caused by Nasopharyngeal Carcinoma Radiotherapy[J].Medical Information,2020,33(11):56.[doi:10.3969/j.issn.1006-1959.2020.05.017]
[6]李宇鑫,周 露,郭 旦,等.沙利度胺预防鼻咽癌放射性口腔粘膜炎的效果及对患者血清TNF-α的影响[J].医学信息,2021,34(01):149.[doi:10.3969/j.issn.1006-1959.2021.01.040]
　LI Yu-xin,ZHOU Lu,GUO Dan,et al.The Effect of Thalidomide in Preventing Radiation-induced Oral Mucositis of Nasopharyngeal Carcinoma and Its Influence on Patients’ Serum TNF-α[J].Medical Information,2021,34(11):149.[doi:10.3969/j.issn.1006-1959.2021.01.040]
[7]陆柔凤,葛 微.鼻咽癌影响因素研究热点与前沿分析[J].医学信息,2022,35(22):34.[doi:10.3969/j.issn.1006-1959.2022.22.006]
　LU Rou-feng,GE Wei.Research Focus and Frontier Analysis of Influencing Factors of Nasopharyngeal Carcinoma[J].Medical Information,2022,35(11):34.[doi:10.3969/j.issn.1006-1959.2022.22.006]
[8]林彩星,陈 罡,杨振东,等.汉黄芩素抑制鼻咽癌细胞增殖、迁移和侵袭能力的研究[J].医学信息,2023,36(10):71.[doi:10.3969/j.issn.1006-1959.2023.10.017]
　LIN Cai-xing,CHEN Gang,YANG Zhen-dong,et al.Effects of Wogonin on Proliferation, Migration and Invasion of Nasopharyngeal Carcinoma Cells[J].Medical Information,2023,36(11):71.[doi:10.3969/j.issn.1006-1959.2023.10.017]
[9]俞丹菁,廖永强,夏洪娇.血清IL-6、干扰素-γ、表皮生长因子在鼻咽癌患者中的表达及意义[J].医学信息,2024,37(02):107.[doi:10.3969/j.issn.1006-1959.2024.02.020]
　YU Dan-jing,LIAO Yong-qiang,XIA Hong-jiao.Expression and Significance of Serum IL-6, Interferon-γ and Epidermal Growth Factor in Patients with Nasopharyngeal Carcinoma[J].Medical Information,2024,37(11):107.[doi:10.3969/j.issn.1006-1959.2024.02.020]

更新日期/Last Update: 1900-01-01