[1]宋昭君,骆 娟,郑佳状,等.载拉帕替尼靶向超声微泡联合高强度聚焦超声对HER-2阳性人乳腺癌SK-BR-3细胞作用的实验研究[J].医学信息,2024,37(10):76-82.[doi:10.3969/j.issn.1006-1959.2024.10.015]
　SONG Zhao-jun,LUO Juan,ZHENG Jia-zhuang,et al.Experimental Study on the Effect of Lapatinib-loaded Targeted Ultrasound Microbubbles Combined with High-intensity Focused Ultrasound on HER-2 Positive Human Breast Cancer SK-BR-3 Cells[J].Journal of Medical Information,2024,37(10):76-82.[doi:10.3969/j.issn.1006-1959.2024.10.015]
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载拉帕替尼靶向超声微泡联合高强度聚焦超声对HER-2阳性人乳腺癌SK-BR-3细胞作用的实验研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年10期
页码:
76-82
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Experimental Study on the Effect of Lapatinib-loaded Targeted Ultrasound Microbubbles Combined with High-intensity Focused Ultrasound on HER-2 Positive Human Breast Cancer SK-BR-3 Cells
文章编号:
1006-1959（2024）10-0076-07
作者:
宋昭君; 骆 娟; 郑佳状; 等
（遂宁市中心医院骨科中心，四川 遂宁 629000）
Author(s):
SONG Zhao-jun; LUO Juan; ZHENG Jia-zhuang; et al.
(Spine Surgery Department of Suining Central Hospital,Suining 629000,Sichuan,China)
关键词:
超声微泡; 高强度聚焦超声; 拉帕替尼; 乳腺癌
Keywords:
Ultrasonic microbubbles; High-intensity focused ultrasound; Rapatinib; Breast cancer
分类号:
R9737.9
DOI:
10.3969/j.issn.1006-1959.2024.10.015
文献标志码:
A
摘要:
目的 探讨携HER-2抗体载拉帕替尼新型靶向纳米超声微泡联合高强度聚焦超声（HIFU）对人乳腺癌SK-BR-3细胞的生物学作用。方法 采用机械振荡法制备携HER-2抗体载拉帕替尼新型靶向纳米超声微泡体外培养HER-2（+）人乳腺癌SK-BR-3细胞珠，分为4组。HER-2抗体载拉帕替尼超声微泡为A组、单纯载拉帕替尼超声微泡+HER-2抗体为B组、携HER-2抗体空白超声微泡为C组、空白超声微泡为D组。载拉帕替尼靶向纳米超声微泡与细胞联合培养，联合HIFU作用，采用流式细胞技术、CCK-8实验、western blot实验在不同时间点观察载拉帕替尼靶向纳米超声微泡联合HIFU对SK-BR-3细胞生物学效应。结果 本实验所制备的携HER-2抗体载拉帕替尼新型靶向纳米超声微泡的平均粒径为（204.80±116.30）nm，Zeta电位为（-5.42±4.11）mV，包封率为（35.20±1.58）%。体外细胞寻靶实验结果显示，携HER-2抗体载拉帕替尼新型靶向纳米超声微泡联合HIFU作用与SK-BR-3细胞的结合能力高于其他三组（P＜0.05）；流式细胞结果显示A组能有效促进SK-BR-3细胞凋亡，CCK-8实验结果显示A组能明显抑制SK-BR-3细胞增殖，同时A组细胞凋亡相关蛋白Bcl-2表达升高，侵袭相关蛋白MMP-2表达降低（P＜0.05）。结论 携HER-2抗体载拉帕替尼超声微泡联合HIFU消融作用能有效地将拉帕替尼递送入SK-BR-3细胞，并能有效抑制SK-BR-3细胞的生长，促进其凋亡。
Abstract:
Objective To explore the effect of lapatinib-loaded targeted ultrasound microbubbles combined with high-intensity focused ultrasound(HIFU) on HER-2 positive human breast cancer SK-BR-3 cells.Methods The HER-2 (+) human breast cancer SK-BR-3 cell beads were cultured in vitro by using a novel targeted ultrasound microbubbles carrying HER-2 antibody and lapatinib-loaded by mechanical oscillation method, which were divided into 4 groups. HER-2 antibody and lapatinib-loaded targeted ultrasound microbubbles were group A, simple lapatinib-loaded targeted ultrasound microbubbles+HER-2 antibody were group B, HER-2 antibody and blank-loaded ultrasound microbubbles were group C, and blank ultrasound microbubbles were group D. Lapatinib-loaded targeted ultrasound microbubbles were co-cultured with cells and combined with HIFU.Flow cytometry, CCK-8 assay and western blot assay were used to observe the biological effects of Lapatinib-loaded targeted ultrasound microbubbles combined with HIFU on SK-BR-3 cells at different time points.Results The mean diameter of the lapatinib-loaded targeted ultrasound microbubbles was(204.8±116.3)nm, the Zeta potential was (-5.42±4.11)mV, and the encapsulation efficiency was (35.20±1.58)%. The results of in vitro cell targeting experiments showed that the binding ability of the lapatinib-loaded targeted ultrasound microbubbles with HER-2 antibody and HIFU to SK-BR-3 cells was higher than that of the other three groups (P<0.05). The results of flow cytometry showed that group A could effectively promote the apoptosis of SK-BR-3 cells. The results of CCK-8 assay showed that group A could significantly inhibit the proliferation of SK-BR-3 cells. At the same time, the expression of apoptosis-related protein Bcl-2 increased and the expression of invasion-related protein MMP-2 decreased in group A (P<0.05).Conclusion Lapatinib-loaded targeted ultrasound microbubbles with HER-2 antibody and HIFU can effectively deliver lapatinib to SK-BR-3 cells, effectively inhibit the growth of SK-BR-3 cells, and promote their apoptosis. Lapatinib-loaded targeted ultrasound microbubbles combined with HIFU ablation will provide a new and safe method for the treatment of breast cancer and its metastasis.

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更新日期/Last Update: 1900-01-01