[1]马亚文,Bingling Xu,刘丽华.水通道蛋白1与非小细胞肺癌的相关性研究[J].医学信息,2022,35(06):73-76.[doi:10.3969/j.issn.1006-1959.2022.06.018]
　MA Ya-wen,Bingling Xu,LIU Li-hua.Study on the Correlation Between AQP1 and Non-small Cell Lung Cancer[J].Medical Information,2022,35(06):73-76.[doi:10.3969/j.issn.1006-1959.2022.06.018]
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水通道蛋白1与非小细胞肺癌的相关性研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年06期
页码:
73-76
栏目:
论著
出版日期:
2022-03-15

文章信息/Info

Title:
Study on the Correlation Between AQP1 and Non-small Cell Lung Cancer
文章编号:
1006-1959（2022）06-0073-04
作者:
马亚文; Bingling Xu; 刘丽华
（1.广西医科大学第一附属医院呼吸内科，广西 南宁 530021；2.Royal Brompton Hospital，England London SW3 6LY）
Author(s):
MA Ya-wen; Bingling Xu; LIU Li-hua
(1.Department of Respiratory Medicine,the First Affiliated Hospital of Guangxi Medical University,Nanning 530000,Guangxi,China;2.Royal Brompton Hospital,London SW3 6LY,England)
关键词:
水通道蛋白1; 非小细胞肺癌; 胸膜组织
Keywords:
Aquaporin 1; Non-small cell lung cancer; Pleural tissue
分类号:
R563
DOI:
10.3969/j.issn.1006-1959.2022.06.018
文献标志码:
A
摘要:
目的 探讨水通道蛋白-1（AQP1）在体外对非小细胞肺癌细胞的影响及AQP1在非小细胞肺癌胸膜组织中的表达情况，分析其潜在应用价值。方法 收集广西医科大学第一附属医院2020年5月-2021年10月内科胸腔镜下组织标本60例，其中非小细胞肺癌胸膜组织标本40例，良性胸腔胸膜组织标本20例进行石蜡包埋切片，采用免疫组化S-P法测定AQP1的表达；通过转染慢病毒及筛选，获得过表达AQP1的稳定株，利用细胞增殖实验检测转染过表达AQP1慢病毒对A549细胞增殖能力的影响，通过流式细胞仪检测抑制AQP1对A549细胞凋亡能力的影响。结果 免疫组化显示，AQP1在非小细胞肺癌胸膜组织中呈高表达，高于良性胸膜组织，差异有统计学意义（P＜0.05）；细胞增殖实验和凋亡实验显示，与正常细胞相比过表达AQP1可以增强A549细胞的增殖能力，抑制AQP1的表达则促进了A549细胞早期和晚期的凋亡率。结论 AQP1在非小细胞肺癌患者胸膜组织中高表达且AQP1可能通过抑制凋亡而促进肺癌细胞增殖。
Abstract:
Objective To explore the effect of Aquaporin1 on non-small cell lung cancer cells in vitro and the expression of AQP1 in pleural tissues of non-small cell lung cancer, and to analyze its potential clinical application value.Methods A total of 60 cases of thoracoscopic tissue specimens were collected from the First Affiliated Hospital of Guangxi Medical University from May 2020 to October 2021, including 40 cases of non-small cell lung cancer pleural tissue specimens and 20 cases of benign pleural tissue specimens. Paraffin-embedded sections were used to detect the expression of AQP1 by immunohistochemical S-P method. The stable AQP1 overexpression strain was obtained by transfecting lentivirus and screening. The effect of AQP1 overexpression lentivirus on the proliferation of A549 cells was detected by cell proliferation assay. The effect of AQP1 inhibition on the apoptosis of A549 cells was detected by flow cytometry.Results Immunohistochemistry showed that AQP1 was highly expressed in pleural tissue of non-small cell lung cancer, which was higher than that in benign pleural tissue (P<0.05). Cell proliferation assay and apoptosis assay showed that overexpression of AQP1 could enhance the proliferation of A549 cells compared with normal cells. Inhibition of AQP1 expression promoted the early and late apoptosis of A549 cells.Conclusion AQP1 is highly expressed in pleural tissue of patients with non-small cell lung cancer and AQP1 may promote the proliferation of lung cancer cells by inhibiting apoptosis.

参考文献/References:

[1]Siegel RL,Miller KD,Jemal A.Cancer statistics,2020[J].CA Cancer J Clin,2020,70(1):7-30.[2]Wei X,Dong J.Aquaporin 1 promotes the proliferation and migration of lung cancer cell in vitro[J].Oncology Reports,2015,34(3):1440-1448.[3]Pennell NA,Arcila ME,Gandara DR,et al.Biomarker Testing for Patients With Advanced Non-Small Cell Lung Cancer: Real-World Issues and Tough Choices[J].Am Soc Clin Oncol Educ Book,2019(39):531-542.[4]Yun S,Sun PL,Jin Y,et al.Aquaporin 1 Is an Independent Marker of Poor Prognosis in Lung Adenocarcinoma[J].J Pathol Transl Med,2016,50(4):251-257.[5]Kong B,Zhao S,Kang X,et al.MicroRNA-133a-3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1[J].Oncology Letters,2021,22(3):1-10.[6]Palethorpe HM,Tomita Y,Smith E,et al.The Aquaporin 1 Inhibitor Bacopaside II Reduces Endothelial Cell Migration and Tubulogenesis and Induces Apoptosis[J].International Journal of Molecular Sciences,2018,19(3):653.[7]Wang Z,Wang Y,He Y,et al.Aquaporin-1 facilitates proliferation and invasion of gastric cancer cells via GRB7-mediated ERK and Ras activation [J].Animal Cells and Systems,2020,24(5):253-259.[8]Wei M,Yu H,Cai C,et al.MiR-3194-3p Inhibits Breast Cancer Progression by Targeting Aquaporin 1 [J].Frontiers in Oncology,2020(10):1513.[9]张羿,朱正秋,薛静.外周血中性粒细胞与淋巴细胞比值预测晚期非小细胞肺癌免疫治疗疗效的价值[J].现代肿瘤医学,2022,30(1):54-57.[10]韩忠诚,王凤霞,马丽丽,等.miR-409-3p调控靶基因Beclin-1抑制小细胞肺癌细胞的自噬进而抑制其生长和转移[J].现代肿瘤医学,2022,30(4):570-575.[11]Li S,Zhou K,Wang M,et al.Degree of pulmonary fissure completeness can predict postoperative cardiopulmonary complications and length of hospital stay in patients undergoing video-assisted thoracoscopic lobectomy for early-stage lung Cancer[J].Interactive Cardiovascular and Thoracic Surgery,2018,26(1):25-33.[12]Huo Z,Lomora M,Kym U,et al.AQP1 Is Up-Regulated by Hypoxia and Leads to Increased Cell Water Permeability, Motility, and Migration in Neuroblastoma[J].Frontiers in cell and Developmental Biology,2021(9):224.[13]Yoshida T,Hojo S,Sekine S,et al.Expression of aquaporin-1 is a poor prognostic factor for stage II and III colon cancer[J].Molecular and Clinical Oncology,2013,1(6):953-958.[14]Abdelrahman AE,Ibrahim DA,El-Azony A,et al.ERCC1, PARP-1, and AQP1 as predictive biomarkers in colon cancer patients receiving adjuvant chemotherapy[J].Cancer Biomarkers,2020,27(2):251-264.[15]Tomita Y,Dorward H,Yool AJ,et al.Role of Aquaporin 1 Signalling in Cancer Development and Progression[J].International Journal of Molecular Sciences,2017,18(2):299.[16]Zhang Y,Qu H.Expression and clinical significance of aquaporin-1, vascular endothelial growth factor and microvessel density in gastric cancer[J].Medicine,2020,99(36):e21883.[17]Traberg-Nyborg L,Login FH,Edamana S,et al.Aquaporin-1 in breast cancer[J].APMIS,2022,130(1):3-10.[18]Zou W,Yang Z,Li D,et al.AQP1 and AQP3 Expression are Associated With Severe Symptoms and Poor-prognosis of the Pancreatic Ductal Adenocarcinoma[J].Appl Immunohistochem Mol Morphol,2019,27(1):40-47.[19]Wu Z,Li S,Liu J,et al.RNAi-mediated silencing of AQP1 expression inhibited the proliferation, invasion and tumorigenesis of osteosarcoma cells[J].Cancer Biology & Therapy,2015,16(9):1332-1340.[20]Bellezza G,Vannucci J,Bianconi F,et al.Prognostic implication of aquaporin 1 overexpression in resected lung adenocarcinoma[J].Interact Cardiovasc Thorac Surg,2017,25(6):856-861.[21]Simone L,Gargano CD,Pisani F,et al.Aquaporin-1 inhibition reduces metastatic formation in a mouse model of melanoma [J].J Cell Mol Med,2018,22(2):904-912.

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更新日期/Last Update: 1900-01-01