[1]于官正,李 鸿,涂 星.基于网络药理学与GEO基因芯片探讨四君子汤与痛泻要方“同病异治”腹泻型肠易激综合征的作用机制[J].医学信息,2023,36(06):14-19,24.[doi:10.3969/j.issn.1006-1959.2023.06.003]
　YU Guan-zheng,LI Hong,TU Xing.Explore the Mechanism of Sijunzi Decoction and Tongxieyaofang for" Same Disease with Different Treatments" of Diarrhea Predominant-irritable Bowel Syndrome Based on Network Pharmacology and GEO Gene Chip[J].Journal of Medical Information,2023,36(06):14-19,24.[doi:10.3969/j.issn.1006-1959.2023.06.003]
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基于网络药理学与GEO基因芯片探讨四君子汤与痛泻要方“同病异治”腹泻型肠易激综合征的作用机制()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年06期
页码:
14-19,24
栏目:
中医药信息学
出版日期:
2023-03-15

文章信息/Info

Title:
Explore the Mechanism of Sijunzi Decoction and Tongxieyaofang for" Same Disease with Different Treatments" of Diarrhea Predominant-irritable Bowel Syndrome Based on Network Pharmacology and GEO Gene Chip
文章编号:
1006-1959（2023）06-0014-07
作者:
于官正; 李 鸿; 涂 星
（1.湖北民族大学医学部，湖北 恩施 445000；2.湖北民族大学武陵山中药材检验检测中心，湖北 恩施 445000）
Author(s):
YU Guan-zheng; LI Hong; TU Xing
(1.Medical Department of Hubei Minzu University,Enshi 445000,Hubei,China;2.Chinese Medicinal Materials Products Quality Supevision and Inspection Center in Wuling Mountainous Area,Hubei Minzu University,Enshi 445000,Hubei,China)
关键词:
网络药理学; GEO基因芯片; 四君子汤; 痛泻要方; 腹泻型肠易激综合征; 同病异治
Keywords:
Network pharmacology; GEO gene chip; Sijunzi decoction; Tongxieyaofang; Diarrhea diarrhea predominant-irritable bowel syndrome; Same disease with different treatments
分类号:
R285
DOI:
10.3969/j.issn.1006-1959.2023.06.003
文献标志码:
A
摘要:
目的 基于网络药理学与GEO基因芯片挖掘，并预测四君子汤与痛泻要方同病异治抗腹泻型肠易激综合征（IBS-D）的共性作用靶点及相关信号通路，研究其作用机制。方法 GEO数据库筛选IBS-D患者差异表达基因作为疾病靶点；中药系统药理学分析平台（TCMSP）数据库以OB≥30%，DL≥0.18为条件筛选，获取四君子汤与痛泻要方活性成分及对应靶点；将IBS-D疾病靶点与四君子汤和痛泻要方靶点做Venn图取交集，获得交集基因，构建中药复方调控网络；将交集基因录入STRING数据库绘制PPI网络，筛选核心基因；运行R语言对交集基因进行富集分析。结果 GEO数据库载有IBS-D差异表达基因2642个；TCMSP数据库载有四君子汤有效成分与靶点为136、229个，痛泻要方有效成分与靶点为43、129个；四君子汤和痛泻要方与IBS-D交集基因共36个，包含23个共性基因。GO与KEGG富集分析结果显示，脂质与动脉粥样硬化信号通路、AGE-RAGE信号通路、脂多糖反应、营养水平反应等为两复方治疗IBS-D潜在共性靶点。结论 脂质与动脉粥样硬化信号通路、AGE-RAGE信号通路、脂多糖反应、营养水平反应可能为四君子汤与痛泻要方发挥“同病异治”抗IBS-D作用的共性靶点。
Abstract:
Objective To predict the common targets, related signal pathways and mechanism of Sijunzi decoction and Tongxieyaofang in treating diarrhea predominant-irritable bowel syndrome (IBS-D) with concept of "same disease with different treatments" based on the network pharmacology method and GEO gene chip.Methods GEO database was used to screen the differentially expressed genes of IBS-D patients as disease targets. The active ingredients and corresponding targets of Sijunzi decoction and Tongxieyaofang were selected by the TCMSP database under the conditions of OB≥30% and DL≥0.18. The intersection of the IBS-D disease targets with the targets of Sijunzi decoction and Tongxieyaofang main prescription was made by Venn diagram, and the intersection genes were obtained to construct the regulatory network of traditional Chinese medicine compound. The intersection genes were imported into STRING database to draw PPI network, and the core genes were screened. R language was used for enrichment analysis of intersection genes.Results There were 2642 IBS-D differentially expressed genes in GEO database. The TCMSP database contained 136 active ingredients and 229 and targets of Sijunzi decoction, and 43 active ingredients and 129 and targets of Tongxieyaofang. There were 36 intersections between Sijunzi decoction, Tongxieyaofang and IBS-D, including 23 common genes. GO and KEGG enrichment analysis showed that lipid and atherosclerosis signaling pathway, AGE-RAGE signaling pathway, lipopolysaccharide response, and nutrient level response were potential common targets for IBS-D treatment.Conciusion Lipid and atherosclerosis signaling pathway, AGE-RAGE signaling pathway, lipopolysaccharide response, and nutrient level response may be the common targets for Sijunzi decoction and Tongxieyaofang to exert the anti-IBS-D effects of the same disease and different treatment.

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更新日期/Last Update: 1900-01-01