[1]高 灿,赵晓彤,程 江.结核分枝杆菌TB10.4蛋白的生物信息学分析[J].医学信息,2023,36(18):1-7.[doi:10.3969/j.issn.1006-1959.2023.18.001]
　GAO Can,ZHAO Xiao-tong,CHENG Jiang.Bioinformatics Analysis of Mycobacterium Tuberculosis TB10.4 Protein[J].Journal of Medical Information,2023,36(18):1-7.[doi:10.3969/j.issn.1006-1959.2023.18.001]
点击复制

结核分枝杆菌TB10.4蛋白的生物信息学分析()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年18期
页码:
1-7
栏目:
生物信息学
出版日期:
2023-09-15

文章信息/Info

Title:
Bioinformatics Analysis of Mycobacterium Tuberculosis TB10.4 Protein
文章编号:
1006-1959（2023）18-0001-07
作者:
高 灿; 赵晓彤; 程 江
（1.石河子大学医学院，新疆 石河子 832008；2.石河子大学医学院第一附属医院检验科，新疆 石河子 832008）
Author(s):
GAO Can; ZHAO Xiao-tong; CHENG Jiang
(1.Shihezi University School of Medicine,Shihezi 832008,Xinjiang,China;2.Department of Clinical Laboratory,the First Affiliated Hospital of Shihezi University School of Medicine,Shihezi 832008,Xinjiang,China)
关键词:
结核分枝杆菌; ESAT-6蛋白; TB10.4蛋白; 生物信息学分析
Keywords:
Mycobacterium tuberculosis; ESAT-6 protein; TB10.4 protein; Bioinformatics analysis
分类号:
R378.91+1
DOI:
10.3969/j.issn.1006-1959.2023.18.001
文献标志码:
A
摘要:
目的 预测分析结核分枝杆菌Rv0288基因的编码蛋白TB10.4的结构和功能。方法 采用生物信息学软件ORF Finder、ExPASy、PSORT、SOSUI、TMHMM、SOPMA等预测分析结核分枝杆菌TB10.4蛋白的结构和功能。结果 TB10.4蛋白由96个氨基酸组成，分子式为C448H677N125O143S9，原子总数1402，编码基因全长291 bp，等电点4.58，不稳定性50.36，为不稳定性蛋白。ProtScale软件预测TB10.4蛋白的脂肪族氨基酸指数为59.38，平均疏水性-0.266。SOSUI和Signal IP软件预测该蛋白无信号肽无跨膜区。TMHMM、NerNGlyc、NetPhos软件预测TB10.4蛋白为可溶性蛋白，有8个磷酸化位点，无糖基化位点。TB10.4蛋白的二级结构以α-螺旋（86.46%）为主，亚细胞定位于细胞质。IEDB软件预测该蛋白有3个B细胞抗原表位；SYFPEITHI、RANKPEP、NetMHC软件预测该蛋白有多个CTL表位和Th表位。STRING预测TB10.4和esxS、esxB、esxH、esxG、PPE4、PPE 18、PE5等蛋白有相互作用，参与多种胞浆蛋白，其二级结构生物学过程。结论 TB10.4蛋白为不稳定疏水性以α-螺旋结构为主，含有多个T、B细胞抗原位点，能够磷酸化并与多种蛋白相互作用，可作为抗结核疫苗的候选蛋白。
Abstract:
Objective To predict and analyze the structure and function of the protein TB10.4 encoded by Rv0288 gene of Mycobacterium tuberculosis.Methods The structure and function of TB10.4 protein of Mycobacterium tuberculosis were predicted and analyzed by bioinformatics software ORF Finder, ExPASy, PSORT, SOSUI, TMHMM and SOPMA.Results TB10.4 protein was composed of 96 amino acids, the molecular formula was C448H677N125O143S9, the total number of atoms was 1402, the coding gene was 291 bp, the isoelectric point was 4.58, and the instability was 50.36, which was an unstable protein. ProtScale software predicted that the aliphatic amino acid index of TB10.4 protein was 59.38, and the average hydrophobicity was -0.266. SOSUI and SignalIP software predicted that the protein had no signal peptide and no transmembrane region. TMHMM, NerNGlyc and NetPhos software predicted that TB10.4 protein was a soluble protein with 8 phosphorylation sites and no glycosylation sites. The secondary structure of TB10.4 protein was mainly α-helix (86.46%), and subcellular localization was in the cytoplasm. The IEDB software predicted that the protein had three B-cell epitopes; SYFPEITHI, RANKPEP, and NetMHC software predicted that the protein had multiple CTL epitopes and Th epitopes. STRING predicted that TB10.4 interacted with proteins such as esxS, esxB, esxH, esxG, PPE4, PPE 18, PE5 and participated in a variety of cytoplasmic proteins and their secondary structure biological processes.Conclusion TB10.4 protein is unstable and hydrophobic, mainly with α-helix structure, containing multiple T and B cell antigen sites, which can be phosphorylated and interact with a variety of proteins, and can be used as a candidate protein for anti-tuberculosis vaccine.

参考文献/References:

[1]Glaziou P,Floyd K,Raviglione MC.Global Epidemiology of Tuberculosis[J].Semin Respir Crit Care Med,2018,39(3):271-285.[2]Bagcchi S.WHO’s Global Tuberculosis Report 2022[J].Lancet Microbe,2023,4(1):e20.[3]Soto JA,Gálvez NMS,Andrade CA,et al.BCG vaccination induces cross-protective immunity against pathogenic microorganisms[J].Trends Immunol,2022,43(4):322-335.[4]Kim YS,Yang CS,Nguyen LT,et al.Mycobacterium abscessus ESX-3 plays an important role in host inflammatory and pathological responses during infection[J].Microbes Infect,2017,19(1):5-17.[5]Granados-Tristán AL,Borrego-Loya A,González-Escalante LA,et al.Role of esxG and esxH Genes in Drug-Resistant Mycobacterium[J].Microb Drug Resist,2020,26(11):1279-1290.[6]Tinaztepe E,Wei JR,Raynowska J,et al.Role of Metal-Dependent Regulation of ESX-3 Secretion in Intracellular Survival of Mycobacterium tuberculosis[J].Infect Immun,2016,84(8):2255-2263.[7]Li X,Chen L,Liao J,et al.A novel stress-inducible CmtR-ESX3-Zn2+ regulatory pathway essential for survival of Mycobacterium bovis under oxidative stress[J].J Biol Chem,2020,295(50):17083-17099.[8]Sun X,Yu W,Pang Q,et al.Conjugation Reaction with 8-Arm PEG Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein[J].Bioconjug Chem,2017,28(6):1658-1668.[9]Sergeeva M,Romanovskaya-Romanko E,Zabolotnyh N,et al.Mucosal Influenza Vector Vaccine Carrying TB10.4 and HspX Antigens Provides Protection against Mycobacterium tuberculosis in Mice and Guinea Pigs[J].Vaccines (Basel),2021,9(4):394.[10]Vasilyev K,Shurygina AP,Zabolotnykh N,et al.Enhancement of the Local CD8+ T-Cellular Immune Response to Mycobacterium tuberculosis in BCG-Primed Mice after Intranasal Administration of Influenza Vector Vaccine Carrying TB10.4 and HspX Antigens[J].Vaccines (Basel),2021,9(11):1273.[11]Moule MG,Cirillo JD.Mycobacterium tuberculosis Dissemination Plays a Critical Role in Pathogenesis[J].Front Cell Infect Microbiol,2020,10:65.[12]Krasniqi S,Jakupi A,Daci A,et al.Tuberculosis Treatment Adherence of Patients in Kosovo[J].Tuberc Res Treat,2017,2017:4850324.[13]Sommer MOA,Munck C,Toft-Kehler RV,et al.Prediction of antibiotic resistance: time for a new preclinical paradigm? [J].Nat Rev Microbiol,2017,15(11):689-696.[14]Korol CB,Shallom SJ,Arora K,et al.Tissue specific diversification, virulence and immune response to Mycobacterium bovis BCG in a patient with an IFN-γ R1 deficiency[J].Virulence,2020,11(1):1656-1673.[15]Dowdy D.Refocusing the timeline of tuberculosis through the lens of BCG[J].Thorax,2021,76(11):1071.[16]Schrager LK,Chandrasekaran P,Fritzell BH,et al.WHO preferred product characteristics for new vaccines against tuberculosis[J].Lancet Infect Dis,2018,18(8):828-829.[17]Gonzalo-Asensio J,Marinova D,Martin C,et al.MTBVAC: Attenuating the Human Pathogen of Tuberculosis (TB) Toward a Promising Vaccine against the TB Epidemic[J].Front Immunol,2017,8:1803.[18]Moreira M,Ruggiero A,Esposito L,et al.Structural features of HtpGMtb and HtpG-ESAT6Mtb vaccine antigens against tuberculosis: Molecular determinants of antigenic synergy and cytotoxicity modulation [J].Int J Biol Macromol,2020,158:305-317.[19]Belogorodtsev SN,Nemkova EK,Stavitskaya NV,et al.Pathogenic Effects of M. tuberculosis-Specific Proteins ESAT-6 and CFP-10 in Macrophage Culture and in 3D-Granulemogenesis Model In Vitro[J].Bull Exp Biol Med,2021,171(5):656-660.[20]Lightbody KL,Ilghari D,Waters LC,et al.Molecular features governing the stability and specificity of functional complex formation by Mycobacterium tuberculosis CFP-10/ESAT-6 family proteins[J].J Biol Chem,2008,283(25):17681-17690.[21]Yu W,Shen L,Qi J,et al.Conjugation with loxoribine and mannan improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein[J].Eur J Pharm Biopharm,2022,172:193-202.[22]Lee YH,Hyun YS,Jo HA,et al.Comprehensive analysis of mycobacterium tuberculosis antigen-specific CD4+ T cell responses restricted by single HLA class Ⅱ allotype in an individual[J].Front Immunol,2022,13:897781.

相似文献/References:

[1]刘德福.肺结核患者痰中结核分枝杆菌检验的临床价值分析[J].医学信息,2018,31(01):151.[doi:10.3969/j.issn.1006-1959.2018.01.059]
　LIU De-fu.Clinical Value Analysis of Mycobacterium tuberculosis in Sputum of Patients with Pulmonary Tuberculosis[J].Journal of Medical Information,2018,31(18):151.[doi:10.3969/j.issn.1006-1959.2018.01.059]
[2]陈 波,马晓波,洪国粦.Xpert MTB/RIF技术对结核分枝杆菌的诊断价值[J].医学信息,2019,32(06):172.[doi:10.3969/j.issn.1006-1959.2019.06.056]
　CHEN Bo,MA Xiao-bo,HONG Guo-lin.Diagnostic Value of Xpert MTB/RIF Technique for Mycobacterium Tuberculosis[J].Journal of Medical Information,2019,32(18):172.[doi:10.3969/j.issn.1006-1959.2019.06.056]
[3]胡立珍,周广信,霍丽丽,等.全自动半巢式实时荧光定量PCR技术检测 BALF诊断肺结核的价值[J].医学信息,2019,32(15):165.[doi:10.3969/j.issn.1006-1959.2019.15.056]
　HU Li-zhen,ZHOU Guang-xin,HUO Li-li,et al.Detection of the Value of BALF in the Diagnosis of Pulmonary Tuberculosis by Automatic Semi-nested Real-time PCR[J].Journal of Medical Information,2019,32(18):165.[doi:10.3969/j.issn.1006-1959.2019.15.056]
[4]李 鑫.肺结核合并气管支气管结核危险因素及诊断[J].医学信息,2020,33(01):32.[doi:10.3969/j.issn.1006-1959.2020.01.012]
　LI Xin.Risk Factors and Diagnosis of Tuberculosis with Tracheobronchial Tuberculosis[J].Journal of Medical Information,2020,33(18):32.[doi:10.3969/j.issn.1006-1959.2020.01.012]
[5]曾冬梅.血清中结核分枝杆菌IgG、IgM抗体检测在肺结核诊断中的应用价值[J].医学信息,2022,35(22):157.[doi:10.3969/j.issn.1006-1959.2022.22.039]
　ZENG Dong-mei.The Application Value of Serum Mycobacterium Tuberculosis IgG and IgM Antibody Detection in the Diagnosis of Pulmonary Tuberculosis[J].Journal of Medical Information,2022,35(18):157.[doi:10.3969/j.issn.1006-1959.2022.22.039]
[6]徐 玥,王 璞.吸烟对肺结核发病、临床特征及转归的影响[J].医学信息,2021,34(05):54.[doi:10.3969/j.issn.1006-1959.2021.05.016]
　XU Yue,WANG Pu.The Effect of Smoking on the Incidence,Clinical Characteristics and Outcome of Tuberculosis[J].Journal of Medical Information,2021,34(18):54.[doi:10.3969/j.issn.1006-1959.2021.05.016]
[7]杨晓悦,李毓龙,罗仲秋,等.结合分枝杆菌感染者辅助性Th2型细胞因子IL-4、IL-6、IL-10的水平变化[J].医学信息,2023,36(01):113.[doi:10.3969/j.issn.1006-1959.2023.01.022]
　YANG Xiao-yue,LI Yu-long,LUO Zhong-qiu,et al.Changes of Helper Th2 Cytokines IL-4, IL-6 and IL-10 Levels in Patients with Mycobacterium Tuberculosis Infection[J].Journal of Medical Information,2023,36(18):113.[doi:10.3969/j.issn.1006-1959.2023.01.022]
[8]于 江,孙 杰,刘凤君.结核分枝杆菌抗原在结核病病理学诊断中的研究进展[J].医学信息,2023,36(08):170.[doi:10.3969/j.issn.1006-1959.2023.08.038]
　YU Jiang,SUN Jie,LIU Feng-jun.Research Progress of Mycobacterium Tuberculosis Antigen in Pathological Diagnosis of Tuberculosis[J].Journal of Medical Information,2023,36(18):170.[doi:10.3969/j.issn.1006-1959.2023.08.038]
[9]黄 丹,王 璞.肉芽肿性肺疾病的研究现状[J].医学信息,2022,35(05):44.[doi:10.3969/j.issn.1006-1959.2022.05.011]
　HUANG Dan,WANG Pu.Research Status of Granulomatous Lung Disease[J].Journal of Medical Information,2022,35(18):44.[doi:10.3969/j.issn.1006-1959.2022.05.011]
[10]逄亚宁,王 聪,刘 奇,等.结核分枝杆菌BfrA蛋白的生物信息学分析[J].医学信息,2022,35(06):81.[doi:10.3969/j.issn.1006-1959.2022.06.020]
　PANG Ya-ning,WANG Cong,LIU Qi,et al.Bioinformatics Analysis of BfrA Protein in Mycobacterium Tuberculosis[J].Journal of Medical Information,2022,35(18):81.[doi:10.3969/j.issn.1006-1959.2022.06.020]

更新日期/Last Update: 1900-01-01