[1]白玉芬,徐洪涛,尹太勇,等.维生素D对系统性红斑狼疮鼠Peyer小结Tfh细胞微环境的影响[J].医学信息,2020,33(13):49-53.[doi:10.3969/j.issn.1006-1959.2020.13.014]
　BAI Yu-fen,XU Hong-tao,YIN Tai-yong,et al.The Effect of Vitamin D on the Microenvironment of Tfh Cells in Peyer’s Nodules of Systemic Lupus Erythematosus[J].Medical Information,2020,33(13):49-53.[doi:10.3969/j.issn.1006-1959.2020.13.014]
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维生素D对系统性红斑狼疮鼠Peyer小结Tfh细胞微环境的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年13期
页码:
49-53
栏目:
论著
出版日期:
2020-07-01

文章信息/Info

Title:
The Effect of Vitamin D on the Microenvironment of Tfh Cells in Peyer’s Nodules of Systemic Lupus Erythematosus
文章编号:
1006-1959（2020）13-0049-05
作者:
白玉芬; 徐洪涛; 尹太勇; 等
（解放军中部战区总医院儿科，湖北 武汉 430070）
Author(s):
BAI Yu-fen; XU Hong-tao; YIN Tai-yong; et al
(Department of Pediatrics,Central War Theater General Hospital,Wuhan 430070,Hubei,China)
关键词:
维生素D; SLE; 滤泡辅助性T细胞; Peyer小结
Keywords:
Vitamin D; SLE; Follicular helper T cells; Peyer’s summary
分类号:
R692.3
DOI:
10.3969/j.issn.1006-1959.2020.13.014
文献标志码:
A
摘要:
目的 研究维生素D（VitD）在系统性红斑狼疮（SLE）治疗中的作用机制。方法 将6只C57/B6小鼠随机分为两组（组1、2），12只MRL/lpr小鼠随机分为四组（组3~6），每组3只，其中组1、6不做处理，组2：C57/B6小鼠+VitD[10 ng/（kg·d）]；组3：MRL/lpr小鼠+VitD[10 ng/（kg·d）]；组4：MRL/lpr小鼠+VitD[30 ng/（kg·d）]；组5：MRL/lpr小鼠+VitD[10 ng/（kg·d）]+PA （0.8 μg/kg，3/W）。灌胃6周后处死小鼠，收集Peyer小结，流式细胞仪测定活化及凋亡的Tfh细胞百分率，B淋巴细胞及浆细胞百分率，趋化因子CCL12、CXCL13、CCL19、CCL21的表达；Real-Time PCR测定IL-21、IL-4mRNA的表达；Western Blot测定IL-21、IL-4、Bcl-6、Blimp-1、XBP-1、Pax-5、BAFF蛋白的表达。结果 与SLE鼠组相比，给予VitD可以降低Tfh细胞百分率但不能明显改变Tfh细胞的活化，减少细胞凋亡，降低B淋巴细胞和浆细胞百分率。同时可以下调IL-21、IL-4、Blimp-1、XBP-1、BAFF蛋白，细胞因子IL-21、IL-4mRNA和趋化因子CCL12、CXCL13、CCL19、CCL21的表达，上调Bcl-6、Pax-5蛋白的表达，且呈剂量依赖性，与PA联用效果更佳。结论 VitD与其受体结合可能通过抑制Tfh细胞、相关因子及微环境、抑制自身免疫抗体的生成，从而对SLE产生治疗作用。
Abstract:
Objective To study the mechanism of vitamin D (VitD) in the treatment of systemic lupus erythematosus (SLE).Methods Six C57/B6 mice were randomly divided into two groups (groups 1 and 2), and 12 MRL/lpr mice were randomly divided into four groups (groups 3 to 6), with 3 mice in each group, of which groups 1 and 6 without treatment, Group 2: C57/B6 mice + VitD[10 ng/(kg·d)]; Group 3: MRL/lpr mice+VitD[10 ng/(kg·d)]; Group 4: MRL /lpr mice+VitD[30 ng/(kg·d)]; Group 5: MRL/lpr mice+VitD[10 ng/(kg·d)]+PA (0.8 μg/kg, 3/W). After 6 weeks of gavage, the mice were sacrificed, Peyer nodules were collected, flow cytometry was used to determine the percentage of activated and apoptotic Tfh cells, B lymphocytes and plasma cells, and the expression of chemokines CCL12, CXCL13, CCL19, CCL21; Real-Time PCR measured the expression of IL-21 and IL-4 mRNA; Western Blot measured the expression of IL-21, IL-4, Bcl-6, Blimp-1, XBP-1, Pax-5 and BAFF protein.Results Compared with the SLE mice group, the administration of VitD can reduce the percentage of Tfh cells but can not significantly change the activation of Tfh cells, reduce apoptosis, and reduce the percentage of B lymphocytes and plasma cells. At the same time, it can down-regulate the expression of IL-21, IL-4, Blimp-1, XBP-1, BAFF protein, cytokines IL-21, IL-4mRNA and chemokines CCL12, CXCL13, CCL19, CCL21, and up-regulate Bcl-6,the expression of Pax-5 protein is dose-dependent, and it is more effective in combination with PA.Conclusion The binding of VitD to its receptor may have a therapeutic effect on SLE by inhibiting Tfh cells, related factors and microenvironment, and inhibiting the production of autoimmune antibodies.

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更新日期/Last Update: 1900-01-01