[1]王亚婷,刘 昱,李明权.基于网络药理学探讨金钱草治疗肾结石的作用机制[J].医学信息,2022,35(02):69-74.[doi:10.3969/j.issn.1006-1959.2022.02.017]
　WANG Ya-ting,LIU Yu,LI Ming-quan.Mechanism of Herba Lysimachiae on Renal Calculi Based on Network Pharmacology[J].Medical Information,2022,35(02):69-74.[doi:10.3969/j.issn.1006-1959.2022.02.017]
点击复制

基于网络药理学探讨金钱草治疗肾结石的作用机制()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年02期
页码:
69-74
栏目:
论著
出版日期:
2022-01-15

文章信息/Info

Title:
Mechanism of Herba Lysimachiae on Renal Calculi Based on Network Pharmacology
文章编号:
1006-1959（2022）02-0069-06
作者:
王亚婷1; 刘 昱1; 李明权2
1.成都中医药大学临床医学院，四川 成都 610075；2.成都中医药大学附属医院肾病二科，四川 成都 610075
Author(s):
WANG Ya-ting1; LIU Yu1; LI Ming-quan2
1.School of Clinical Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan,China;2.The Second Department of Nephrology,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan,China
关键词:
金钱草; 肾结石; 网络药理学
Keywords:
Lysimachia; Nephrolithiasis; Network pharmacology
分类号:
R692.4
DOI:
10.3969/j.issn.1006-1959.2022.02.017
文献标志码:
A
摘要:
目的 基于网络药理学探讨金钱草治疗肾结石的可能分子机制。方法 基于TCMSP、SwissTargetPrediction数据库及Uniprot数据库筛选出金钱草的有效成分及靶点基因，通过GeneCards数据库及OMIM数据库筛选出肾结石的疾病靶点基因，用Cytoscape软件构建“药物-活性成分-疾病-靶点”相互作用网络图，将有效成分靶标与疾病靶标上传到String数据库，构建蛋白互作用网络图（PPI），并计算其特征值，使用R语言对得到的PPI进行核心基因的筛选，运用R语言对关键靶点进行基因本体（GO） 富集分析和京都基因与基因组百科全书（KEGG）通路富集分析。结果 共筛选得到金钱草10个活性成分，涉及82个作用靶点；网络分析显示，靶点主要涉及NF-κappB结合、热休克蛋白结合、核受体活性、转录因子活性，直接的配体调节序列特异性DNA结合、类固醇结合、组蛋白激酶活性等生物学过程；富集分析显示，相关基因主要通过调节前列腺癌、Apoptosis、MAPK、p5等信号通路来发挥治疗肾结石的作用。结论 金钱草中的槲皮素、山柰酚、金合欢素可能是其治疗肾结石的物质基础，其作用机制涉及了NF-κappB结合、热休克蛋白结合、核受体活性、转录因子活性，直接的配体调节序列特异性DNA结合、类固醇结合、组蛋白激酶活性等。
Abstract:
Objective To explore the possible molecular mechanism of Lysimachia in the treatment of renal calculi based on network pharmacology.Methods The effective components and target genes of Herba Lysimachiae were screened based on TCMSP, SwissTargetPrediction database and Uniprot database. The disease target genes of kidney stones were screened by GeneCards database and OMIM database. The "drug-active components-disease-target" interaction network diagram was constructed by Cytoscape software. The effective components and disease targets were uploaded to the String database. The protein interaction network diagram (PPI) was constructed and the eigenvalues were calculated. R language was used to screen the core genes of PPI, and R language was used to perform gene ontology (GO) enrichment analysis of key targets and Kyoto gene and genome encyclopedia (KEGG) pathway enrichment analysis.Results Ten active components of Lysimachia christinae were screened,involving 82 action targets.Network analysis showed that the target mainly involved in NF-κappB binding, heat shock protein binding, nuclear receptor activity, transcription factor activity, direct ligand regulatory sequence specific DNA binding, steroid binding, histone kinase activity and other biological processes ; enrichment analysis showed that related genes mainly played a role in the treatment of renal calculi by regulating prostate cancer, apoptosis, MAPK, p5 and other signaling pathways.Conclusion Quercetin, kaempferol and acacia in S. mandshurica may be the material basis for its treatment of renal calculi. Its mechanism involves NF-κappB binding, heat shock protein binding, nuclear receptor activity, transcription factor activity, and direct ligand regulation of sequence-specific DNA binding, steroid binding, and histone kinase activity.

参考文献/References:

[1]罗冬珍,吴昕,卢利员,等.泌尿系结石与中医体质类型相关性研究[J].内蒙古中医药,2015(6):121.[2]陈菊飞,陈红波.B超评估中药排石汤治疗肾结石的临床疗效分析[J].中华中医药学刊,2015,33(2):373-374.[3]杨程.中西医结合治疗泌尿系结石的临床疗效[J].包头医学院学报,2016,32(9):114-115.[4]邓律胤,吕纪华,王丽,等.广金钱草总黄酮片对大鼠肾结石的作用[J].世界中西医结合杂志,2019,14(9):1252-1255.[5]国家中医药管理局,《中华本草》编委会.中华本草全10册[M].上海:上海科学技术出版社,2018.[6]李军,蔡泓,王君明,等.金钱草化学成分、药理作用及临床应用[J].中国老年学杂志,2017,37(24):6262-6264. [7]蔡华芳.金钱草治疗尿结石的作用和机理研究[J].中国医疗前沿,2010,5(12):7-8.[8]叶苗,刘丽仙,梁晓美.槲皮素对大鼠肾缺血再灌注损伤模型的保护作用[J].中国中西医结合肾病杂志,2019,20(12):1041-1043.[9]Zhu W,Xu YF,Feng Y,et al.Prophylactic effects of quercetin and hyperoside in a calcium oxalate stone forming rat model[J].Urolithiasis,2014,42(6):519-526. [10]Jiang W,Wang R,Liu D,et al.Protective Effects of Kaempferitrin on Advanced Glycation End Products Induce Mesangial Cell Apoptosis and Oxidative Stress[J].Int J Mol Sci,2018,19(11):3334. [11]Ali BH,Za’abi MA,Karaca T,et al.Potassium bromate-induced kidney damage in rats and the effect of gum acacia thereon[J].Am J Transl Res,2018,10(1):126-137. [12]Mitsui Y,Chang I,Fukuhara S,et al.CYP1B1 promotes tumorigenesis via altered expression of CDC20 and DAPK1 genes in renal cell carcinoma[J].BMC Cancer,2015(15):942.[13]Montford JR,Bauer C,Dobrinskikh E,et al.Inhibition of 5-lipoxygenase decreases renal fibrosis and progression of chronic kidney disease[J].Am J Physiol Renal Physiol,2019,316(4):732-742.[14]Zhu W,Zhao Z,Chou FJ,et al.The Protective Roles of Estrogen Receptorβ in Renal Calcium Oxalate Crystal Formation via Reducing the Liver Oxalate Biosynthesis and Renal Oxidative Stress-Mediated Cell Injury[J].Oxid Med Cell Longev,2019:5305014. [15]Barrera-Chimal J,Pérez-Villalva R,Ortega JA,et al.Intra-renal transfection of heat shock protein 90 alpha or beta (Hsp90α or Hsp90β) protects against ischemia/reperfusion injury[J].Nephrol Dial Transplant,2014,29(2):301-312.[16]Jiang H,Gao X,Gong J,et al.Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress[J].Oxid Med Cell Longev,2018:1724648. [17]Lu Y,Qin B,Hu H,et al.Integrative microRNA-gene expression network analysis in genetic hypercalciuric stone-forming rat kidney[J].Peer J,2016(4):e1884. [18]Fong-Ngern K,Sueksakit K,Thongboonkerd V.Surface heat shock protein 90 serves as a potential receptor for calcium oxalate crystal on apical membrane of renal tubular epithelial cells[J].Biol Inorg Chem,2016,21(4):463-474. [19]Ilbey YO,Ozbek E,Simsek A,et al.Effects of pomegranate juice on hyperoxaluria-induced oxidative stress in the rat kidneys[J].Ren Fail,2009,31(6):522-531.[20]Miyazawa K,Suzuki K,Ikeda R,et al.Apoptosis and its related genes in renal epithelial cells of the stone-forming rat[J].Urol Res,2005,33(1):31-38.

相似文献/References:

[1]杨 恒,钱 彪,王勤章,等.Claudin-14在两种肾结石模型肾组织中表达的对比研究[J].医学信息,2022,35(09):87.[doi:10.3969/j.issn.1006-1959.2022.09.022]
　YANG Heng,QIAN Biao,WANG Qin-zhang,et al.Comparative Study of Claudin-14 Expression in Renal Tissues of Two Renal Calculi Models[J].Medical Information,2022,35(02):87.[doi:10.3969/j.issn.1006-1959.2022.09.022]
[2]钟愉明,宋乐明,彭作锋,等.智能控压输尿管软镜吸引取石术治疗肾结石的临床研究[J].医学信息,2018,31(24):87.[doi:10.3969/j.issn.1006-1959.2018.24.023]
　ZHONG Yu-ming,SONG Le-ming,PENG Zuo-feng,et al.Clinical Study of Intelligent Pressure-controlled Ureteroscope for Attracting Stone to Treat Renal Calculi[J].Medical Information,2018,31(02):87.[doi:10.3969/j.issn.1006-1959.2018.24.023]
[3]郝志强,王勤章,钱 成,等.纳米细菌肾结石模型血、尿生化的动态观测[J].医学信息,2019,32(11):58.[doi:10.3969/j.issn.1006-1959.2019.11.017]
　HAO Zhi-qiang,WANG Qin-zhang,QIAN Cheng,et al.Dynamic Observation of Blood and Urine Biochemistry in Nanobacteria Kidney Stone Model[J].Medical Information,2019,32(02):58.[doi:10.3969/j.issn.1006-1959.2019.11.017]
[4]邓廷志,李富林,陈烈钳,等.输尿管软镜碎石术与经皮肾镜碎石术治疗 直径≤2 cm肾结石的临床疗效对比[J].医学信息,2019,32(13):91.[doi:10.3969/j.issn.1006-1959.2019.13.025]
　DENG Ting-zhi,LI Fu-lin,CHEN Lie-qian,et al.Comparison of Clinical Efficacy between Ureteroscopic Lithotripsy and Percutaneous Nephrolithotomy for Renal Calculi with Diameter≤2 cm[J].Medical Information,2019,32(02):91.[doi:10.3969/j.issn.1006-1959.2019.13.025]
[5]吴晓丽,刘 峰,谢建军.输尿管软镜联合钬激光碎石术在肾结石合并2型糖尿病中的效果[J].医学信息,2020,33(06):103.[doi:10.3969/j.issn.1006-1959.2020.06.030]
　WU Xiao-li,LIU Feng,XIE Jian-jun.Effect of Soft Ureteroscope Combined with Holmium Laser Lithotripsy in Kidney Stones Combined with Type 2 Diabetes[J].Medical Information,2020,33(02):103.[doi:10.3969/j.issn.1006-1959.2020.06.030]
[6]黄郁兰.延续性护理联合知信行健康教育模式对肾结石术后患者复发风险的影响[J].医学信息,2023,36(21):158.[doi:10.3969/j.issn.1006-1959.2023.21.037]
　HUANG Yu-lan.Effect of Continuous Nursing Combined with Knowledge-attitude-practice Health Education Model on Recurrence Risk of Patients with Renal Calculi After Operation[J].Medical Information,2023,36(02):158.[doi:10.3969/j.issn.1006-1959.2023.21.037]
[7]张 鹏.肾结石患者尿钙、镁、钠、钾与尿阳离子的相关性分析[J].医学信息,2021,34(18):110.[doi:10.3969/j.issn.1006-1959.2021.18.028]
　ZHANG Peng.Correlation Analysis of Urinary Calcium,Magnesium,Sodium,Potassium andUrinary Cations in Patients with Kidney Stones[J].Medical Information,2021,34(02):110.[doi:10.3969/j.issn.1006-1959.2021.18.028]
[8]吴旭峰,孙 发.肾结石发生机制及外科治疗研究[J].医学信息,2021,34(23):25.[doi:10.3969/j.issn.1006-1959.2021.23.007]
　WU Xu-feng,SUN Fa.Study on Mechanism and Surgical Treatment of Renal Calculi[J].Medical Information,2021,34(02):25.[doi:10.3969/j.issn.1006-1959.2021.23.007]
[9]聂岳龙,欧阳汛,周 亮,等.微创经皮肾镜取石术治疗肾积水合并肾结石的临床实践及结石清除率观察[J].医学信息,2023,36(13):95.[doi:10.3969/j.issn.1006-1959.2023.13.018]
　NIE Yue-long,OUYANG Xun,ZHOU Liang,et al.Clinical Practice and Stone Clearance Rate of Minimally Invasive Percutaneous Nephrolithotomy in the Treatment of Nephrolithiasis Complicated with Renal Calculi[J].Medical Information,2023,36(02):95.[doi:10.3969/j.issn.1006-1959.2023.13.018]
[10]孙 硕.腰麻联合硬膜外麻醉与全身麻醉在输尿管软镜肾结石钬激光碎石术中的有效性和安全性[J].医学信息,2023,36(20):121.[doi:10.3969/j.issn.1006-1959.2023.20.023]
　SUN Shuo.Efficacy and Safety of Combined Spinal-epidural Anesthesia and General Anesthesia in Ureteroscopic Holmium Laser Lithotripsy for Renal Calculi[J].Medical Information,2023,36(02):121.[doi:10.3969/j.issn.1006-1959.2023.20.023]

更新日期/Last Update: 1900-01-01