[1]杨 恒,钱 彪,王勤章,等.Claudin-14在两种肾结石模型肾组织中表达的对比研究[J].医学信息,2022,35(09):87-89,115.[doi:10.3969/j.issn.1006-1959.2022.09.022]
　YANG Heng,QIAN Biao,WANG Qin-zhang,et al.Comparative Study of Claudin-14 Expression in Renal Tissues of Two Renal Calculi Models[J].Medical Information,2022,35(09):87-89,115.[doi:10.3969/j.issn.1006-1959.2022.09.022]
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Claudin-14在两种肾结石模型肾组织中表达的对比研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年09期
页码:
87-89,115
栏目:
论著
出版日期:
2022-05-01

文章信息/Info

Title:
Comparative Study of Claudin-14 Expression in Renal Tissues of Two Renal Calculi Models
文章编号:
1006-1959（2022）09-0087-04
作者:
杨 恒; 钱 彪; 王勤章; 等
（1.石河子大学医学院第一附属医院泌尿外科，新疆 石河子 8320082.赣南医学院第一附属医院泌尿外科，江西 赣州 341000）
Author(s):
YANG Heng; QIAN Biao; WANG Qin-zhang; et al.
(1.Department of Urology,the First Affiliated Hospital of Medical College of Shihezi University,Shihezi 832008,Xinjiang,China;2.Department of Urology,the First Affiliated Hospital of Gannan Medical College,Ganzhou 341000,Jiangxi,China)
关键词:
肾结石; 纳米细菌; 乙二醇; 大鼠肾结石模型; Claudin-14
Keywords:
Kidney stones; Nanobacteria; Ethylene glycol; Rat kidney stone model; Claudin-14
分类号:
R692.4
DOI:
10.3969/j.issn.1006-1959.2022.09.022
文献标志码:
A
摘要:
目的 通过观察Claudin-14在两种实验性大鼠肾结石模型中表达的差异，探究纳米细菌在结石形成过程中的作用。方法 选取90只Wistar雄性大鼠，随机分为纳米细菌组（NB组，大鼠尾静脉注射NB+生理盐水灌胃）、乙二醇组（EG组，大鼠1%乙二醇饮水和2%氯化铵AC 2 ml灌胃2周）、空白对照组（NC组，大鼠尾静脉注射生理盐水+生理盐水灌胃），人工采集大鼠血、尿进行钙浓度检测，取大鼠肾脏组织行结石成分分析及免疫组化检测Claudin-14改变。结果 NB组、EG组含草酸钙结石分别占总结石数的61.54%、78.95%；NB组与EG组1~10周内血钙、尿钙含量分别比较，差异无统计学意义（P＞0.05）；EG组第3周后Claudin-14蛋白的表达显著增强，NB组第4周后Claudin-14蛋白的表达开始增强，EG组Claudin-14蛋白的表达显著强于NB组，但NC组Claudin-14蛋白的表达未见明显改变。结论 纳米细菌是形成结石的原因之一，草酸钙结晶的形成不仅有纳米细菌的参与，同时Claudin-14所参与的细胞通路也起到了相应的作用，导致尿钙升高，最终形成结石。
Abstract:
Objective To investigate the role of nanobacteria in the formation of renal calculi by observing the difference of Claudin-14 expression in two experimental rat renal calculi models.Methods A total of 90 male Wistar rats were randomly divided into nanobacteria group (NB group, rats were intragastrically administrated with NB+normal saline via tail vein), ethylene glycol group (EG group, rats were intragastrically administrated with 1% ethylene glycol drinking water and 2% ammonium chloride AC 2 ml for 2 weeks), and blank control group (NC group, rats were intragastrically administrated with normal saline via tail vein+normal saline). The blood and urine of rats were collected artificially for calcium concentration detection. The kidney tissues of rats were taken for stone composition analysis and immunohistochemical detection of Claudin-14 changes.Results Calcium oxalate stones in NB group and EG group accounted for 61.54% and 78.95% of the total stones, respectively. There was no significant difference in blood calcium and urine calcium between NB group and EG group within 1-10 weeks (P>0.05). The expression of Claudin-14 protein in EG group was significantly increased after 3 weeks, and the expression of Claudin-14 protein in NB group began to increase after 4 weeks; the expression of Claudin-14 protein in EG group was significantly stronger than that in NB group, but there was no significant change in NC group.Conclusion Nanobacteria are one of the reasons for the formation of stones. The formation of calcium oxalate crystals is not only involved in nanobacteria, but also in the cell pathway involved in Claudin-14, leading to the increase of urinary calcium and the formation of stones.

参考文献/References:

[1]Claesson-Welsh L,Dejana E,Mcdonald DM.Permeability of the Endothelial Barrier: Identifying and Reconciling Controversies[J].Trends in Molecular Medicine,2021,27(4):314-331.[2]Nagpal R,Yadav H.Bacterial Translocation from the Gut to the Distant Organs: An Overview[J].Annals of Nutrition and Metabolism,2017,71(1):11-16.[3]Arcidiacono T,Simonini M,Lanzani C,et al.Claudin-14 Gene Polymorphisms and Urine Calcium Excretion[J].Clin J Am Soc Nephrol,2018,13(10):1542-1549.[4]褚浩,王勤章,吴双,等.纳米细菌大鼠肾结石模型肾脏结石形成时间的动态研究[J].中国全科医学,2017,20(21):2613-2618.[5]江帆,闫亮.河南地区345例泌尿系结石临床分析[J].河南外科学杂志,2019,25(2):42-44.[6]杨军.间苯三酚治疗急诊泌尿系结石致肾绞痛的效果[J].中国药物经济学,2018,13(5):44-46.[7]Ye Z,Zeng G,Yang H,et al.The status and characteristics of urinary stone composition in China[J].BJU Int,2020,125(6):801-809.[8]Grant C,Guzman G,Stainback RP,et al.Variation in Kidney Stone Composition Within the United States[J].Journal of endourology,2018,32(10):973-977.[9]杨博.52-Hz小分子团水对乙二醇诱导的大鼠肾结石的预防作用及其机制的研究[D].重庆:第三军医大学,2016.[10]刘兆飞,黄永斌,张海涛,等.低压灌注超声碎石清石治疗感染性肾结石42例疗效观察[J].中国微创外科杂志,2017,17(6):568-570.[11]李天,孙祥宙,盛明,等.输尿管软镜负压鞘在输尿管软镜治疗感染性肾结石术中的应用[J].实用医学杂志,2018,34(1):79-82.[12]余骏川,邓耀良,黎承杨,等.羟基磷灰石对人肾小管上皮细胞骨桥蛋白表达的影响及机制探讨[J].山东医药,2017,57(14):6-9.[13]曾春晖,李先梅,蔡妮娜,等.草酸钙型肾结石小鼠模型的研究[J].世界中医药,2016,11(11):2213-2215.[14]王敬珅,郝志强,杨恒,等.氧自由基在纳米细菌致肾结石形成过程中的作用[J].吉林医学2020,41(9),2053-2057.[15]崔庆鹏,刘孝东,范世成,等.沉默钙敏感受体对大鼠尿钙排泄影响及对紧密连接蛋白14的作用[J].实用医学杂志2020,36(14):1882-1885,1891.[16]吴昆旻,李泽卿,朱春晖,等.紧密连接蛋白在声带白斑伴咽喉反流患者声带黏膜中的表达[J].中国耳鼻咽喉颅底外科杂志,2017,23(6):541-545.[17]Nasim F,Sabath BF,Eapen GA.Lung cancer[J].Med Clin North Am,2019,103(3):463-473.[18]Liu S,Zhao Z,Wang D,et al.Decreased FOXF1 promotes hepatocellular carcinoma tumorigenesis, invasion, and stemness and is associated with poor clinical outcome[J].Oncotargets&Therapy,2016,9(1):1743-1752.[19]Argyrou A,Legaki E,Koutserimpas C,et al.Risk factors for gastroesophageal reflux disease and analysis of genetic contributors[J].World J Clin Cases,2018,6(8):176-182.[20]Elkouby NL,Abassi Z,Lagziel A,et al.Double gene deletion reveals lack of cooperation between claudin 11 and claudin 14 tight junction proteins [J].Cell and tissue research,2008,333(3):427-438.

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更新日期/Last Update: 1900-01-01