[1]郝志强,王勤章,钱 成,等.纳米细菌肾结石模型血、尿生化的动态观测[J].医学信息,2019,32(11):58-62,71.[doi:10.3969/j.issn.1006-1959.2019.11.017]
　HAO Zhi-qiang,WANG Qin-zhang,QIAN Cheng,et al.Dynamic Observation of Blood and Urine Biochemistry in Nanobacteria Kidney Stone Model[J].Journal of Medical Information,2019,32(11):58-62,71.[doi:10.3969/j.issn.1006-1959.2019.11.017]
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纳米细菌肾结石模型血、尿生化的动态观测()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年11期
页码:
58-62,71
栏目:
论著
出版日期:
2019-06-01

文章信息/Info

Title:
Dynamic Observation of Blood and Urine Biochemistry in Nanobacteria Kidney Stone Model
文章编号:
1006-1959（2019）11-0058-06
作者:
郝志强1; 王勤章1; 钱 成2; 徐 浩3; 申茂磊1; 李 朋1; 钱 彪1
1.石河子大学医学院第一附属医院泌尿外科，新疆 石河子 832000； 2.安徽省第二人民医院泌尿外科，安徽 合肥 231100； 3.解放军69235部队卫生队，新疆 奎屯 833200
Author(s):
HAO Zhi-qiang1; WANG Qin-zhang1; QIAN Cheng2; XU Hao3; SHEN Mao-lei1; LI Peng1; QIAN Biao1
1.Department of Urology,the First Affiliated Hospital of Shihezi University School of Medicine, Shihezi 832000,Xinjiang,China; 2.Department of Urology,the Second People's Hospital of Anhui Province,Hefei 231100,Anhui,China; 3.People's Liberation Army 692
关键词:
肾结石; 纳米细菌; 血、尿生化; 肾功; 大鼠肾结石模型
Keywords:
Key words：Kidney stones; Nanobacteria; Blood; urine biochemistry; Kidney function; Rat kidney stone model
分类号:
R692.4；R322
DOI:
10.3969/j.issn.1006-1959.2019.11.017
文献标志码:
A
摘要:
目的 构建纳米细菌Wistar雄性大鼠肾结石模型，动态监测血、尿生化各项指标，旨在为进一步研究纳米细菌在肾结石发生发展过程中所起的作用，提供数据支持，进而为肾结石病因学的下一步探索提供新思路。方法 将60只大鼠随机分为纳米细菌组和空白对照组，每组30只。纳米细菌组给予尾静脉注射1.2 ml NB悬液，空白对照组给予尾静脉注射1.2 ml生理盐水，实验周期为10周，每周每组各处死大鼠3只，处死前采集大鼠血、尿标本，供生化分析，并取两侧鲜活肾脏标本，用于病理结晶情况检测及计算肾体比。结果 自第8周开始，纳米细菌组肾体比值大于空白对照组，差异有统计学意义（P＜0.05）。截至第10周末，纳米细菌组大鼠肾脏晶体阳性11只，空白对照组均为阴性，纳米细菌组大鼠肾脏晶体阳性率高于空白对照组，差异有统计学意义（P＜0.05）。与空白对照组相比，纳米细菌组第4~8周血肌酐、第3~8周血尿酸、第3~9周尿素氮及尿钙水平均升高，第8周纳米细菌组尿pH高于空白对照组差，第6~7周纳米细菌组血钙高于空白对照组，差异均有统计学意义（P＜0.05）。结论 肾体比、结晶阳性率、血肌酐、血尿酸、血尿素、尿钙等重要指标的有效升高，均显示纳米细菌Wistar雄性大鼠肾结石实验模型的成功制备，这为进一步揭示NB在结石形成过程中所起的作用以及后续对肾结石病因学的进一步研究提供了良好的实验基础与数据支持。
Abstract:
Abstract：Objective To construct a kidney model of nano-bacteria Wistar male rats, and to dynamically monitor blood and urine biochemical indicators, in order to further study the role of nanobacteria in the development of kidney stones, provide data support, and then the etiology of kidney stones. The next step is to explore new ideas. Methods 60 rats were randomly divided into a nanobacteria group and a blank control group, with 30 rats in each group. The nanobacterial group was given a 1.2 ml suspension of NB in the tail vein, and the blank control group was given a 1.2 ml saline solution in the tail vein. The experimental period was 10 weeks. Three rats died in each group per week. Blood and urine were collected before sacrifice. Specimens for biochemical analysis, and take fresh kidney specimens on both sides for pathological crystallization and calculation of renal to body ratio. Results Since the 8th week, the renal body ratio of the nanobacterial group was larger than that of the blank control group,the difference was statistically significant (P<0.05). By the end of the 10th week, there were 11 kidney crystals in the nano-bacterial group, and the blank control group was negative. The positive rate of renal crystal in the nano-bacterial group was higher than that in the blank control group,the difference was statistically significant (P<0.05).Compared with the blank control group, the serum creatinine of the nanobacterial group was 4 to 8 weeks.At the 3rd to 8th week, blood uric acid and urea nitrogen and urinary calcium increased at the 3rd to 9th week. At the 8th week, the urine pH of the nanobacterial group was lower than that of the blank control group. The blood calcium level of the nanobacterial group was higher than that of the 6th to 7th week. The blank control group had significant differences (P<0.05). Conclusion The effective increase of renal body ratio, crystallization rate, serum creatinine, blood uric acid, blood urea, urinary calcium and other important indicators have shown the successful preparation of the experimental model of kidney calculus in nano-bacteria Wistar male rats, which further reveals NB. The role played by the formation of stones and the subsequent further study of the etiology of kidney stones provide a good experimental basis and data support.

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更新日期/Last Update: 2019-06-01