[1]段玉彩,魏亚君,王兵玲,等.基于网络药理学探讨地奥心血康联合诺氟沙星治疗心肌梗死合并肺部感染的分子机制[J].医学信息,2023,36(16):8-15.[doi:10.3969/j.issn.1006-1959.2023.16.002]
　DUAN Yu-cai,WEI Ya-jun,WANG Bing-ling,et al.Molecular Mechanism of Di’ao Xinxuekang Combined with Norfloxacin in Treating Myocardial Infarction Complicated with Pulmonary Infection Based on Network Pharmacology[J].Journal of Medical Information,2023,36(16):8-15.[doi:10.3969/j.issn.1006-1959.2023.16.002]
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基于网络药理学探讨地奥心血康联合诺氟沙星治疗心肌梗死合并肺部感染的分子机制()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年16期
页码:
8-15
栏目:
生物信息学
出版日期:
2023-08-15

文章信息/Info

Title:
Molecular Mechanism of Di’ao Xinxuekang Combined with Norfloxacin in Treating Myocardial Infarction Complicated with Pulmonary Infection Based on Network Pharmacology
文章编号:
1006-1959（2023）16-0008-08
作者:
段玉彩; 魏亚君; 王兵玲; 等
（1.联勤保障部队第九四〇医院心血管内科，甘肃 兰州 730000；2.解放军96604部队医院感染科，甘肃 兰州 730000；3.联勤保障部队第九四〇医院神经内科，甘肃 兰州 730000；4.联勤保障部队第九四〇医院信息科，甘肃 兰州 730000）
Author(s):
DUAN Yu-cai; WEI Ya-jun; WANG Bing-ling; et al.
(1.Department of Cardiovasology,No.940 Hospital of the Joint Logistics Support Force of People’s Liberation Army,Lanzhou 730000,Gansu,China;2.Department of Infectious Disease,No.96604 Hospital of People’s Liberation Army,Lanzhou 730000,Gansu,China;3.Department of Neurology,No.940 Hospital of the Joint Logistics Support Force of People’s Liberation Army,Lanzhou 730000,Gansu,China;4.Department of Information,No.940 Hospital of the Joint Logistics Support Force of People’s Liberation Army,Lanzhou 730000,Gansu,China)
关键词:
网络药理学; 地奥心血康; 诺氟沙星; 心肌梗死; 肺部感染
Keywords:
Network pharmacology; Di’ao Xinxuekang; Norfloxacin; Myocardial infarction; Pulmonary infection
分类号:
R542.2+2
DOI:
10.3969/j.issn.1006-1959.2023.16.002
文献标志码:
A
摘要:
目的 采用网络药理学的方法初步探讨地奥心血康联合诺氟沙星治疗心肌梗死合并肺部感染的分子机制。方法 采用ETCM数据库结合相关文献收集黄山药主要成分并通过有机小分子生物活性数据库（PubChem）以及Swiss Target Prediction数据库预测作用靶点；通过Genecards数据库筛选心肌梗死、肺部感染靶点基因；映射后得到地奥心血康联合诺氟沙星治疗心肌梗死合并肺部感染的共有靶点；借助STRING数据库构建靶蛋白相互作用网络；通过DAVID数据库对共同作用靶点进行GO和KEGG富集分析；通过Cytoscape软件对结果进行拓扑分析，并对其作用机制进行初步探讨。结果 共筛选出21个活性成分，267个共有靶点。经GO和KEGG通路富集分析和靶点网络的拓扑分析得到10个主要活性成分；12个核心靶点参与蛋白质结合、ATP结合、相同蛋白质结合等分子功能，作用于信号转导、RNA聚合酶Ⅱ启动子转录的正调控、细胞增殖的正调控等生物过程，从而影响质膜、膜的组成部分、细胞质等细胞组分，通过cGMP-PKG信号通路、JAK-STATA信号通路、C-type lectin receptor信号通路等信号通路对心肌梗死合并肺部感染产生治疗作用。结论 地奥心血康联合诺氟沙星治疗心肌梗死合并肺部感染的药效物质基础可能是Diosgenin（薯蓣皂素）、Norfloxacin（诺氟沙星）、Palmitic acid（棕榈酸）、Dioscoreside A（黄山药皂苷A）、Stigmasterol（豆固醇）、β-Stiosterol（β-谷甾醇）、Deltoside（三角薯蓣皂甙）、26-Desglucoprotodioscin、Dioscin（薯蓣皂苷）、Protodioscin（原薯蓣皂苷）、Progenin Ⅱ，作用于IL2、STAT3、PIK3CA等靶点，通过激活/抑制cGMP-PKG信号通路、JAK-STATA信号通路、C-type lectin receptor信号通路等信号通路对心肌梗死合并肺部感染产生治疗作用。
Abstract:
Objective To explore the molecular mechanism of Di’ao Xinxuekang combined with norfloxacin in the treatment of myocardial infarction complicated with pulmonary infection by network pharmacology.Methods ETCM database and related literature were used to collect the main components of Dioscorea Panthaica Prain et Burkill, and the targets were predicted by PubChem and Swiss Target Prediction database. The target genes of myocardial infarction and pulmonary infection were screened by Genecards database. After mapping, the common targets of Di’aoXinxuekang combined with norfloxacin in the treatment of myocardial infarction complicated with pulmonary infection were obtained. Target protein interaction network was constructed by STRING database. GO and KEGG enrichment analysis were performed for the co-acting targets through DAVID database. Topological analysis was performed by Cytoscape software, and the mechanism of action was preliminarily discussed.Results A total of 21 active components and 267 common targets were screened. After enrichment analysis of GO and KEGG pathways and topological analysis of target network, 10 main active components were obtained. The 12 core targets were involved in molecular functions such as protein binding, ATP binding and identical protein binding, and acted on biological processes such as signal transduction, positive regulation of RNA polymerase Ⅱ promoter transcription and positive regulation of cell proliferation, thereby affecting plasma membrane, membrane components, cytoplasm and other cellular components. The treatment of myocardial infarction complicated with pulmonary infection could be achieved through cGMP-PKG signaling pathway, JAK-STATA signaling pathway, C-type lectin receptor signaling pathway and other signaling pathways.Conclusion The pharmacodynamic material basis of Di’ao Xinxuekang combined with norfloxacin in the treatment of myocardial infarction complicated with pulmonary infection may be Diosgenin, Norfloxacin, Palmitic acid, Dioscoreside A, Stigmasterol, β-Stiosterol, Deltoside, 26-Desglucoprotodioscin, Dioscin, Protodioscin, Progenin Ⅱ. They acted on IL2, STAT3, PIK3CA and other targets, and have therapeutic effects on myocardial infarction complicated with pulmonary infection by activating/inhibiting cGMP-PKG signaling pathway, JAK-STATA signaling pathway, C-type lectin receptor signaling pathway and other signaling pathways.

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更新日期/Last Update: 1900-01-01