[1]耿 佳,刘 芬,曹 莹,等.长链非编码RNAMIAT在2型糖尿病外周血单核细胞中的表达及相关性分析[J].医学信息,2021,34(12):5-8.[doi:10.3969/j.issn.1006-1959.2021.12.002]
　GENG Jia,LIU Fen,CAO Ying,et al.Expression and Correlation Analysis of Long-chain Non-coding RNAMIAT in Peripheral Blood Mononuclear Cells of Type 2 Diabetes Mellitus[J].Medical Information,2021,34(12):5-8.[doi:10.3969/j.issn.1006-1959.2021.12.002]
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长链非编码RNAMIAT在2型糖尿病外周血单核细胞中的表达及相关性分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年12期
页码:
5-8
栏目:
出版日期:
2021-06-15

文章信息/Info

Title:
Expression and Correlation Analysis of Long-chain Non-coding RNAMIAT in Peripheral Blood Mononuclear Cells of Type 2 Diabetes Mellitus
文章编号:
1006-1959（2021）12-0005-04
作者:
耿 佳; 刘 芬; 曹 莹; 等
（1.西安医学院公共卫生学院卫生学教研室，陕西 西安 710021； 2.新疆医科大学第一附属医院动物疾病模型研究重点实验室，新疆 乌鲁木齐 830054； 3.陕西省公共安全医学防控研究中心，陕西 西安 710021）
Author(s):
GENG Jia; LIU Fen; CAO Ying; et al.
(1.Department of Hygiene,School of Public Health,Xi’an Medical University,Xi’an 710021,Shaanxi,China; 2.The Key Laboratory of Animal Disease Model Research,the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054,Xinjiang,China； 3.Research Center for Medical Prevention and Control of Public Safety of Shaanxi Povince,xi’an 710021,Shaanxi,China)
关键词:
2型糖尿病; 外周血单核细胞; 长链非编码RNA
Keywords:
Type 2 diabetesmellitus; Peripheral blood mononuclear cells; Long non-coding RNA
分类号:
R587.1
DOI:
10.3969/j.issn.1006-1959.2021.12.002
文献标志码:
A
摘要:
目的 研究2型糖尿病（T2DM）患者外周血单核细胞中长链非编码RNA心肌梗死相关转录本（MIAT）表达及其T2DM的相关性，评估了lncRNA MIAT作为T2DM生物标志物的潜在应用价值。方法 选取2017年8月~2018年2月在新疆医科大学第一临床附属医院入院进行就诊的100例汉族T2DM患者作为T2DM组，另选100名健康受试者做对照组。分别收集临床资料和外周血，完成血生化指标的测定，用淋巴细胞分离液分离单核细胞，提取总RNA，应用实时荧光定量PCR法检测lncRNA MIAT的表达。评价lncRNA作为T2DM生物标志物的诊断价值，分析相关危险因素。结果 T2DM组外周血单核细胞中lncRNA MIAT的mRNA水平高于对照组（1.90±0.85 vs 1.11±0.66），差异有统计学意义（P＜0.05）；受试者操作特征曲线分析显示，lncRNA MIAT对T2DM具有良好的诊断价值（AUC=0.753，95%CI：0.686~0.820），多元回归分析显示lncRNA MIAT是T2DM的危险因素（OR=1.527，95%CI=1.130~1.919，P=0.004）。lncRNA MIAT的表达与年龄、体重指数、低密度脂蛋白、总胆固醇、三油甘酯、空腹血糖、餐后2h血糖水平正相关，与性别、高血压、高密度脂蛋白水平负相关，其中具有显著相关性的是低密度脂蛋白（r=0.201、P＜0.05）、总胆固醇（r=0.221，P＜0.05）、空腹血糖（r=0.385，P＜0.05），餐后2h血糖（r=0.342，P＜0.05）。结论外周血单核细胞的lncRNA MIAT表达和T2DM正相关，MIAT参与调控糖尿病的病理发生。
Abstract:
Objective To study the expression of long-chain non-coding RNA myocardial infarction-associated transcript (MIAT) in peripheral blood mononuclear cells of patients with type 2 diabetes mellitus (T2DM) and its correlation with T2DM, and to evaluate the potential application value of lncRNA MIAT as a T2DM biomarker.Methods A total of 100 Han patients with T2DM who were admitted to the First Clinical Affiliated Hospital of Xinjiang Medical University from August 2017 to February 2018 were selected as the T2DM group, and another 100 healthy subjects were selected as the control group.Collecting clinical data and peripheral blood separately, complete the determination of blood biochemical indicators, separate monocytes with lymphocyte separation fluid, extract total RNA, and use real-time fluorescent quantitative PCR to detect the expression of lncRNA MIAT.To evaluate the diagnostic value of lncRNA as a biomarker of T2DM, and analyze related risk factors.Results The mRNA level of lncRNA MIAT in peripheral blood mononuclear cells in the T2DM group was higher than that in the control group (1.90±0.85 vs 1.11±0.66),the difference was statistically significant (P＜0.05);Receiver operating characteristic curve analysis showed that lncRNA MIAT has a good diagnostic value for T2DM (AUC=0.753, 95%CI: 0.686-0.820).Multiple regression analysis showed that lncRNA MIAT was a risk factor for T2DM (OR=1.527, 95%CI=1.130-1.919, P=0.004).The expression of lncRNA MIAT was positively correlated with age, body mass index, low-density lipoprotein, total cholesterol, trioleylglycerol, fasting blood glucose, and 2h postprandial blood glucose level, and negatively correlated with gender, hypertension, and high-density lipoprotein level, which had significant correlation was low-density lipoprotein (r=0.201,P＜0.05), total cholesterol (r=0.221,P＜0.05), fasting blood glucose (r=0.385,P＜0.05), and 2h postprandial blood glucose (r=0.342, P＜0.05).Conclusion The expression of lncRNA MIAT in peripheral blood mononuclear cells is positively correlated with T2DM, and MIAT is involved in the regulation of the pathogenesis of diabetes.

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